We narrowed to 3,399 results for: guide rna expression plasmid

1. pAN057

   Plasmid

   #220053

   Purpose

   NMD-reporter plasmid that expresses a a firefly luciferase gene fused to exons 22-27 of the CFTR gene and a synthetic intron (c.3846G>A mutation; W1282X).

   Depositor

   Blake Wiedenheft

   Article

   Nemudraia et al Science. 2024 Apr 25:eadk5518. doi: 10.1

   Insert

   fLuc-CFTR(W1282X, exons 22-27) (CFTR Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   c.3846G>A mutation

   Promoter

   Available since

   May 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKL2315

   Plasmid

   #187876

   Purpose

   Expresses Cre recombinase.

   Depositor

   Kan Wang

   Article

   Aliu et al Plant Biotechnol J. 2022 Jun 11. doi: 10

   Insert

   Cre recombinase
   

   Use

   Cre/Lox

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23107

   Available since

   Aug. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-EGxxFP

   Plasmid

   #50716

   Purpose

   5’ and 3’ EGFP fragments that shares 482bp were placed under ubiquitous CAG promoter. Used for validation of gRNA sequences by DSB mediated EGFP reconstitution.

   Depositor

   Masahito Ikawa

   Article

   Mashiko et al Sci Rep. 2013 Nov 27;3:3355. doi: 10.103

   Insert

   split EGFP
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Feb. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUC19-EAMP

   Plasmid

   #138265

   Purpose

   mCherry acceptor plasmid with iCas9 target site upstream of mCherry to be used in plasmid to plasmid integration reporter assay.

   Depositor

   Xiao Wang

   Article

   Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10

   Insert

   iCas9 target-mCherry
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a-HTLV

   Available since

   March 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. CMV-TO-g0 (FLP-IN)

   Plasmid

   #236120

   Purpose

   Plasmid encoding g0 guide RNA under control of CMV promoter with two TetR binding sites, used to equalize plasmid masses for transfection

   Depositor

   Xiaojing Gao

   Article

   Wolfsberg et al bioRxiv 2025.02.24.639918

   Insert

   g0
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter with two TetR binding sites

   Available since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-RSV-GFP-U6-Rosa26 gRNA (SpyCas9 scaffold)

   Plasmid

   #120296

   Purpose

   AAV vector; encodes GFP as well as a U6-driven Rosa26-targeting gRNA (SpyCas9 scaffold)

   Depositor

   Dominik Niopek

   Article

   Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

   Insert

   Rosa26 gRNA (SpCas9 scaffold)
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. AiP942-pX330-Sncg

   Plasmid

   #181774

   Purpose

   CRISPR/Cas9 guide vector

   Depositor

   Tanya Daigle

   Insert

   Sncg guide
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. AiP926-pX330-Batf3

   Plasmid

   #181772

   Purpose

   CRISPR/Cas9 guide vector

   Depositor

   Tanya Daigle

   Insert

   Batf3 guide
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. AiP928-pX330-Gpr139

   Plasmid

   #181767

   Purpose

   CRISPR/Cas9 guide vector

   Depositor

   Tanya Daigle

   Insert

   Gpr139 guide
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. AiP938-pX330-Slc17a7

    Plasmid

    #181775

    Purpose

    CRISPR/Cas9 guide vector

    Depositor

    Tanya Daigle

    Insert

    Slc17a7 guide
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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