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MXW-PGK-let-7_2A-IRES-GFP

Plasmid

#26545

Depositor

Chang-Zheng Chen

Article

Trujillo et al EMBO J. 2010 Oct 6;29(19):3272-85. doi:

Insert

cel-let-7 (~250nt flanking each side)

Use

Retroviral

Expression

Mammalian

Mutation

SD1 G->A

Available Since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only

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pHWBF06EC

Plasmid

#130827

Purpose

Expression of plant LINC fluorescent fusion protein for cell biology

Depositor

Hank Bass

Article

Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

Insert

SUN2

Use

Gateway entry vector

Tags

mCherry-FLAG-HA

Availability

Academic Institutions and Nonprofits only

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SUN2delCC

Plasmid

#130836

Purpose

Expression of plant LINC fluorescent fusion protein for cell biology

Depositor

Hank Bass

Article

Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

Insert

SUN2

Tags

mCherry-FLAG-HA

Expression

Plant

Mutation

Deletion of coiled coil domain

Promoter

CaMV 35S

Availability

Academic Institutions and Nonprofits only

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SUN2delSUNec

Plasmid

#130847

Purpose

Expression of plant LINC fluorescent fusion protein for cell biology

Depositor

Hank Bass

Article

Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

Insert

SUN2

Use

Gateway entry vector

Tags

mCherry-FLAG-HA

Mutation

Deletion of SUN domain

Availability

Academic Institutions and Nonprofits only

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SUN2delCCec

Plasmid

#130848

Purpose

Expression of plant LINC fluorescent fusion protein for cell biology

Depositor

Hank Bass

Article

Gumber et al J Cell Sci. 2019 Feb 11;132(3). pii: jcs

Insert

SUN2

Use

Gateway entry vector

Tags

mCherry-FLAG-HA

Mutation

Deletion of coiled coil domain

Availability

Academic Institutions and Nonprofits only

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pR26 CAG/GFP Dest

Plasmid

#74281

Purpose

Gene targeting vector for the mouse Rosa26 locus, including a CAG promoter, loxP flanked stop cassette, GFP, for gateway cloning

Depositor

Ralf Kuehn

Article

Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

Insert

Rosa26 5-homology region

Use

Mouse Targeting

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only

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pPEPY-PF6-lacI

Plasmid

#85589

Purpose

Work as template for amplification of Streptococcus pneumoniae adapted lacI gene, which is flanked by up- and downstream sequence for integration in prsA locus in S. pneumoniae

Depositor

Jan-Willem Veening

Article

Liu et al Mol Syst Biol. 2017 May 10;13(5):931. do

Insert

lacI

Expression

Bacterial

Promoter

PF6

Available Since

Feb. 22, 2017

Availability

Academic Institutions and Nonprofits only

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Myc-Axin2

Plasmid

#21279

Depositor

Frank Costantini

Article

Jho et al Mol Cell Biol. 2002 Feb . 22(4):1172-83.

Insert

Axin2 (Axin2 Mouse)

Tags

Myc

Expression

Mammalian

Available Since

June 9, 2009

Availability

Academic Institutions and Nonprofits only
pEvolvR-mCherry-enCas9(D10A)-PolI3M

Plasmid

#249061

Purpose

Expresses nucleus-localized enCas9 (D10A) fused to PolI3M and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.

Depositor

John Dueber, David Schaffer

Article

Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:

Insert

enCas9-PolI3M

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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V246 pCEP 4HA B56beta

Plasmid

#14533

Depositor

David Virshup

Article

Seeling et al Science. 1999 Mar 26. 283(5410):2089-91.

Insert

PPP2R5B B56 beta regulatory subunit of PP2A (PPP2R5B Human)

Tags

4 HA

Expression

Mammalian

Mutation

I346V relative to GenBank ID L42374

Available Since

March 23, 2007

Availability

Academic Institutions and Nonprofits only

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pR26 CAG AsiSI/MluI

Plasmid

#74286

Purpose

Gene targeting vector for the mouse Rosa26 locus, including a CAG promoter and loxP flanked stop cassette, for cloning into AsiSI or Mlui

Depositor

Ralf Kuehn

Article

Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

Insert

Rosa26 5-homology region

Available Since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-Mm.cargo(Cre)

Plasmid

#174862

Purpose

Expresses mouse Peg10 cargoRNA encoding Cre recombinase

Depositor

Feng Zhang

Article

Segel et al Science. 2021 Aug 20;373(6557):882-889.

Insert

Cre flanked by MmPeg10 UTRs

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pDOC-GG

Plasmid

#149377

Purpose

Chassis plasmid for building mutagenesis cassettes by Golden Gate assembly to form a donor plasmid for recombineering by Gene Doctoring

Depositor

Mark Pallen

Article

Thomson et al BMC Biotechnol. 2020 Oct 7;20(1):54. doi

Inserts

Fragment containing a kanamycin resistance cassette
Fragment containing pMB1 origin of replication and an I-SceI recognition sequence
Fragment containing a lacZ⍺ expression cassette flanked by BsaI sites
Fragment containing an I-SceI recognition sequence

Use

Synthetic Biology

Expression

Bacterial

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pXR004: CasRx pre-gRNA cloning backbone

Plasmid

#109054

Purpose

hU6-driven expression of guide RNAs compatible with CasRx. Contains BbsI sites for guide cloning flanked by 5' and 3' full-length DRs

Depositor

Patrick Hsu

Article

Konermann et al Cell. 2018 Mar 8. pii: S0092-8674(18)302

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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hprtSAGFP

Plasmid

#41594

Purpose

In vivo single-strand annealing substrate composed of two truncated GFP genes, separated by a drug selection marker, and flanked by mouse hprt targeting sequences

Depositor

Maria Jasin

Article

Stark et al Mol Cell Biol. 2004 Nov;24(21):9305-16.

Insert

SA-GFP reporter

Use

Mouse Targeting; Dsb repair reporter substrate

Expression

Mammalian

Available Since

Dec. 3, 2012

Availability

Academic Institutions and Nonprofits only

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pEvolvR-enCas9-PolI3M-TBD

Plasmid

#113077

Purpose

Expresses enCas9 fused to PolI3M-TBD in E. coli. contains a BsmbI-flanked GFP cassette for sgRNA cloning.

Depositor

John Dueber, David Schaffer

Article

Halperin et al Nature. 2018 Aug 1. pii: 10.1038/s41586-

Insert

enCas9-PolI3M-TBD

Expression

Bacterial

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pR26 CAG/GFP Asc

Plasmid

#74285

Purpose

Gene targeting vector for the mouse Rosa26 locus, including a CAG promoter, loxP flanked stop cassette and GFP, for cloning into AscI

Depositor

Ralf Kuehn

Article

Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

Insert

Rosa26 5-homology region

Use

Mouse Targeting

Available Since

April 15, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-AKT-PH-GFP

Plasmid

#18836

Depositor

Craig Montell

Article

Kwon et al Mol Cell. 2007 Feb 23. 25(4):491-503.

Insert

AKT-PH domain (AKT1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

contains amino acids 1-148 (compared to GenBank r…

Promoter

CMV

Available Since

July 14, 2008

Availability

Academic Institutions and Nonprofits only

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pSIH1-puro-STAT3 shRNA

Plasmid

#26596

Depositor

Frank Sinicrope

Article

Huang et al Mol Cancer Ther. 2010 Mar . 9(3):742-50.

Insert

STAT3 shRNA (STAT3 Human)

Use

Lentiviral and RNAi

Available Since

Nov. 17, 2010

Availability

Academic Institutions and Nonprofits only

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pBLU-VIPR

Plasmid

#220498

Purpose

Plasmid allowing for potent induction of gRNA using blue light. The plasmid contains VPR-EL222 and the C120 response element followed by mCherry and cloning sites for ribozyme flanked gRNA (RGR).

Depositor

Alexander Espinosa

Article

Pulgarin et al bioRxiv [Preprint]. 2023 Nov 10:2023.11.

Type

Empty backbone

Use

CRISPR; Transposon

Expression

Mammalian

Available Since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

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