We narrowed to 11,128 results for: CHL

1. pGL0_7 [pSa ORI]

   Plasmid

   #198923

   Purpose

   Level 0 part

   Depositor

   Charlie Gilbert, Nili Ostrov, Cultivarium Tools

   Article

   Gilbert et al bioRxiv 2023.05.19.541510

   Insert

   pSa ORI
   

   Use

   Synthetic Biology

   Available Since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGEX6P1-DEST-His

   Plasmid

   #119763

   Purpose

   Allows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained C-terminal 10xHis tag.

   Depositor

   Andrew Jackson, Martin Reijns

   Type

   Empty backbone

   Tags

   10xHis and GST

   Expression

   Bacterial

   Promoter

   tac promoter

   Available Since

   Jan. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pEPQD0CM0296

   Plasmid

   #162305

   Purpose

   Level 0 CDS part for MoClo assembly, AATG-TTCG

   Depositor

   Nicola Patron

   Article

   Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

   Insert

   CDS, superfolder GFP, no stop codon
   

   Use

   Synthetic Biology

   Available Since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pACYC-PLlambda-alkSm4-3

   Plasmid

   #44383

   Depositor

   Hal Alper

   Article

   Reed et al J Biotechnol. 2012 Apr 15;158(3):75-9. d

   Insert

   AlkSm4-3
   

   Use

   Synthetic Biology

   Mutation

   Q410I, S470T, K546E, E775D

   Promoter

   PLlambda

   Available Since

   July 15, 2013

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. pBXCA3H

   Plasmid

   #47072

   Purpose

   FX cloning E.coli expression vector with araBAD promoter and C-terminal Avi-tag, 3C protease site and 10x His tag

   Depositor

   Raimund Dutzler, Eric Geertsma

   Article

   Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

   Type

   Empty backbone

   Tags

   3C protease site (PreScission site), Avi-tag, and…

   Expression

   Bacterial

   Promoter

   araBAD

   Available Since

   Jan. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGWT35S-RBCS1a(1–79)-TagRFP

   Plasmid

   #182586

   Purpose

   Transient expression of RBCS1a(1–79)-TagRFP in plant cell (Plastid/Chloroplast)

   Depositor

   Yutaka Kodama

   Article

   Osaki et al PeerJ. 2017 Sep 7;5:e3779. doi: 10.7717/

   Insert

   RBCS1a(1–79)
   

   Tags

   TagRFP

   Expression

   Plant

   Promoter

   CaMV 35S promoter

   Available Since

   May 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pACλCI

   Plasmid

   #53730

   Purpose

   PlacUV5-directed synthesis of the λCI protein; used as two-hybrid control. For cloning purposes, see Addgene plasmid 53733.

   Depositor

   Ann Hochschild

   Article

   Dove et al Nature. 1997 Apr 10;386(6625):627-30.

   Insert

   bacteriophage lambda CI protein
   

   Expression

   Bacterial

   Promoter

   lac UV5

   Available Since

   June 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDest-663 (attR4-attR2)

   Plasmid

   #161884

   Purpose

   Multisite Gateway (attR4-attR2) vector for lentivirus production

   Depositor

   Dominic Esposito

   Article

   Wall et al Methods Mol Biol. 2014;1116:193-208. doi

   Type

   Empty backbone

   Use

   Lentiviral

   Available Since

   Jan. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEZY19

   Plasmid

   #18668

   Purpose

   pET19b converted Gateway Destination vector for expressing His tagged fusion proteins in bacteria. See note in comments section below regarding the mutated enterokinase site.

   Depositor

   Yu-Zhu Zhang

   Article

   Guo et al Biotechnol J. 2008 Mar . 3(3):370-7.

   Type

   Empty backbone

   Use

   Gateway destination vector

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 26, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCD185

    Plasmid

    #113317

    Purpose

    dCas9, MCP-SoxS_R93A

    Depositor

    Jesse Zalatan

    Article

    Dong et al Nat Commun. 2018 Jun 27;9(1):2489. doi:

    Insert

    dCas9 and MCP-SoxS_R93A
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    R93A

    Available Since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMYO

    Plasmid

    #34626

    Depositor

    Jesse Rinehart, Dieter Söll

    Article

    Park et al Science. 2011 Aug 26;333(6046):1151-4.

    Insert

    Myoglobin
    

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    codon-optimized

    Promoter

    lpp

    Available Since

    Feb. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDonorGFP(Ptr)

    Plasmid

    #175578

    Purpose

    DNA donor for transposition

    Depositor

    Sheng Yang

    Article

    Yang et al Nucleic Acids Res. 2021 Sep 3. pii: 6363

    Insert

    Mini-Tn, GFP cassette
    

    Expression

    Bacterial

    Mutation

    NO

    Available Since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pUPD2-HA-LRR-NEL-C (B3-B4)

    Plasmid

    #211860

    Purpose

    CDS for E3-DART ligase sequence in domestication vector

    Depositor

    Marcela Rojas-Pierce

    Article

    Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

    Insert

    LRR-NEL
    

    Use

    Synthetic Biology

    Tags

    HA

    Available Since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFF745

    Plasmid

    #61450

    Purpose

    IPTG-inducible SCRIBE_kanR(ON)

    Depositor

    Timothy Lu

    Article

    Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

    Insert

    SCRIBE_kanR(ON)
    

    Use

    Synthetic Biology

    Promoter

    pLacO

    Available Since

    Feb. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBTK1050

    Plasmid

    #209540

    Purpose

    BTK Type 1 homology arm for Golden Gate assembly

    Depositor

    Jeffrey Barrick

    Article

    Lariviere et al bioRxiv 2023.09.19.558440

    Insert

    Upstream homology arm for recombination into the S. alvi wkB2 genome w/in the SALWKB2_RS11215/SALWKB2_RS11220 intergenic region
    

    Use

    Synthetic Biology

    Available Since

    Feb. 9, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pBTK1051

    Plasmid

    #209541

    Purpose

    BTK Type 5 homology arm for Golden Gate assembly

    Depositor

    Jeffrey Barrick

    Article

    Lariviere et al bioRxiv 2023.09.19.558440

    Insert

    Recombination into the S. alvi wkB2 genome within the SALWKB2_RS11215/SALWKB2_RS11220 intergenic region
    

    Use

    Synthetic Biology

    Available Since

    Feb. 2, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pNR188

    Plasmid

    #79543

    Purpose

    Output plasmid: contains 16-state register made up of TP901, BxbI, and A118 recognition sites.

    Depositor

    Timothy Lu

    Article

    Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEMF037

    Plasmid

    #157634

    Purpose

    Batis maritima MHT

    Depositor

    Jonathan Silberg

    Article

    Fulk et al ACS Synth Biol. 2020 Nov 20;9(11):3104-3

    Insert

    MHT
    

    Expression

    Bacterial

    Available Since

    May 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTETDuet_M42FG121VDHFR_R166QTS

    Plasmid

    #191298

    Purpose

    Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

    Depositor

    Kimberly Reynolds

    Article

    Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

    Insert

    folA, thyA
    

    Expression

    Bacterial

    Mutation

    M42F/G121V DHFR, R166Q TYMS

    Promoter

    T7 (folA), Tet (thyA)

    Available Since

    June 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBP_sgRNA

    Plasmid

    #190128

    Purpose

    Level 0 MoClo plasmid for Golden Gate cloning of sgRNAs

    Depositor

    Veronica Godoy-Carter

    Article

    Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge