We narrowed to 7,673 results for: Ski

1. pEGFP-FLAG-AKAP-Lbc

   Plasmid

   #67571

   Purpose

   Expresses GFP and FLAG-tagged AKAP-Lbc in mammalian cells

   Depositor

   Graeme Carnegie

   Article

   Burmeister et al J Biol Chem. 2015 May 8;290(19):12058-67

   Insert

   A-kinase anchoring protein Lbc (AKAP13 Human)
   

   Tags

   FLAG and GFP

   Expression

   Mammalian

   Mutation

   R2604G in AKAP

   Available Since

   Nov. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

2. AICSDP-1:PXN-EGFP

   Plasmid

   #87420

   Purpose

   Homology arms and linker-EGFP sequence for C-terminus tagging of human PXN

   Depositor

   Allen Institute for Cell Science

   Article

   Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

   Insert

   PXN Homology Arms with linker-EGFP (PXN Human)
   

   Use

   CRISPR; Donor template

   Tags

   linker-EGFP

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Available Since

   March 15, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pMXS-IRES-Blast mAtg7

   Plasmid

   #173170

   Purpose

   Retroviral vector to express sgRNA resistant mouse Atg7

   Depositor

   Kivanc Birsoy

   Article

   Zhu et al Cell Metab. 2020 Oct 28. pii: S1550-4131

   Insert

   Autophagy Related 7 (Atg7 Mouse)
   

   Use

   Retroviral

   Mutation

   Synonymous mutations at sgRNA sites

   Promoter

   MMLV

   Available Since

   Feb. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCIBN-EYFP-hLaminB1

   Plasmid

   #103800

   Purpose

   BLInCR 'Localizer' construct that marks the nuclear lamina and is targeted by a PHR-tagged effector upon illumination with blue light

   Depositor

   Karsten Rippe

   Article

   Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

   Insert

   CIBN-EYFP-hLaminB1 (CIB1, LMNB1 Mustard Weed, Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. EGFP-Cyclin B1

   Plasmid

   #215049

   Purpose

   Expresses EGFP-Cyclin B1 in mammalian cells from a retroviral vector.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   CCNB1 (CCNB1 Human)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   LTR

   Available Since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLKO-Tet-On-PLXNB2-shRNA1

   Plasmid

   #98399

   Purpose

   Lentivirus for expression of shRNA1 against human PLEXIN-B2 (Dox-inducible)

   Depositor

   Roland Friedel

   Article

   Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

   Insert

   Plexin-B2 (PLXNB2 Human)
   

   Use

   Lentiviral

   Available Since

   July 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDY0207 ACTB atgRNA with v1 scaffold

   Plasmid

   #179107

   Purpose

   ACTB N-term PBS 13 RT 29 Bxb1 AttB 46 atgRNA

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

   Insert

   ACTB N-term PBS 13 RT 29 AttB 46 atgRNA
   

   Use

   CRISPR

   Available Since

   Feb. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBabe.puro.CDK8.KD.flag

   Plasmid

   #19759

   Depositor

   William Hahn

   Article

   Firestein et al Nature. 2008 Sep 25. 455(7212):547-51.

   Insert

   cyclin-dependent kinase 8 (CDK8 Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   D173A (kinase dead)

   Available Since

   Dec. 11, 2008

   Availability

   Academic Institutions and Nonprofits only

9. pCW57.1 blast OFF 3xflag IREB2

   Plasmid

   #169889

   Purpose

   Express 3x flag IREB2, suppressible by doxycycline addition

   Depositor

   Richard Possemato

   Article

   Terzi et al Sci Adv. 2021 May 26;7(22). pii: 7/22/ea

   Insert

   IREB2 (IREB2 Human)
   

   Use

   Lentiviral; Doxycycline mediated suppression

   Tags

   3x Flag

   Expression

   Mammalian

   Mutation

   Silent mutations in sgIREB2_1 and sgIREB2_2 targe…

   Promoter

   TRE

   Available Since

   May 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDNA5/FRT/Halo-Tag7-Geminin(1/110)_P2A_HaloTag9-Cdt(1-100)Cy+

    Plasmid

    #169337

    Purpose

    Expression of the LT-Fucci(SCA) in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    Fucci(SCA) (GMNN, CDT1 Human)
    

    Tags

    HaloTag7-Geminin(1/110) and HaloTag9-Cdt(1-100)Cy…

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Available Since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDNA5/FRT/HaloTag7-Geminin(1/110)_P2A_HaloTag9-Cdt(1-110)Cy-

    Plasmid

    #169338

    Purpose

    Expression of the LT-Fucci(CA) in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    Fucci(CA) (GMNN, CDT1 Human)
    

    Tags

    HaloTag7-Geminin(1/110) and HaloTag9-Cdt(1-110)Cy…

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Available Since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. mCherry-APEX2

    Plasmid

    #215127

    Purpose

    Expresses mCherry-APEX2 in mammalian cells from a retroviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    APEX2 (APEX2 Human)
    

    Use

    Retroviral

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pNL-CEF-CD79B-YFP

    Plasmid

    #187006

    Purpose

    Expression of human CD79B as YFP fusion protein

    Depositor

    Hermann Eibel

    Article

    Sevdali et al Cell Rep. 2022 Jun 28;39(13):111019. doi

    Insert

    CD79B (CD79B Human)
    

    Use

    Lentiviral

    Tags

    YFP

    Available Since

    July 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pQCXIZ GFP PODXL delta IC

    Plasmid

    #202422

    Purpose

    Expression of GFP-tagged PODXL without intracellular domain

    Depositor

    David Bryant

    Article

    Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

    Insert

    PODXL (PODXL Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Mutation

    deleted intracellular domain

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pYX233-b2-DHFR

    Plasmid

    #163759

    Purpose

    Gal-inducible yeast expression of the mitochondrial "clogger" protein b2(167)-DHFR

    Depositor

    Johannes Herrmann

    Article

    Boos et al Nat Cell Biol. 2019 Apr;21(4):442-451. d

    Insert

    b2-DHFR (Dhfr Mouse, Budding Yeast)
    

    Expression

    Yeast

    Mutation

    Amino acids 1-167 of S.c. Cytochrome b2 (Cyb2) fu…

    Promoter

    GAL1

    Available Since

    March 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDY0310 TP901-1 Integrase

    Plasmid

    #179110

    Purpose

    TP901-1 Integrase expression vector

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

    Insert

    TP901-1 Integrase
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDONR223_PYGL_WT_V5

    Plasmid

    #82991

    Purpose

    Gateway Donor vector containing PYGL, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, Matthew Meyerson, David Root

    Article

    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

    Insert

    PYGL (PYGL Human)
    

    Use

    Gateway entry vector

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-T7-ABE8.20m-nSpCas9-P2A-EGFP (KAC1158)

    Plasmid

    #185915

    Purpose

    pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpCas9(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    human codon optimized ABE8.20m-nSpCas9-P2A-EGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9=D10A

    Promoter

    CMV and T7

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. tet-pLKO.neo_shGFP

    Plasmid

    #110470

    Purpose

    Control, silence GFP gene, doxycycline inducible, neomycin selection

    Depositor

    Sandra Martha Gomes Dias

    Article

    Reis et al J Biol Chem. 2019 Jun 14;294(24):9342-93

    Insert

    GFP
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    H1/TO (RNA PolIII)

    Available Since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pT3-EFa1-HA-Jag1

    Plasmid

    #46051

    Depositor

    Xin Chen

    Article

    Tschaharganeh et al Gastroenterology. 2013 Jun;144(7):1530-1

    Insert

    Jag1 (Jag1 Rat)
    

    Tags

    HA tag

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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