We narrowed to 59,538 results for: cloning

1. U6-Nme2_tracr

   Plasmid

   #192146

   Purpose

   Expresses Nme2-sgRNA cloned with BspQI

   Depositor

   Yongming Wang

   Article

   Wei et al Elife. 2022 Aug 12;11. pii: 77825. doi:

   Insert

   Nme2 sgRNA scaffold
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pICSL70004

   Plasmid

   #50334

   Depositor

   Nicola Patron

   Article

   Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

   Insert

   kanamycin/neomycin/paromomycin resistance cassette (Nos promoter (A. tumefaciens) + NptII (E.coli) + Ocs terminator (A. tumefaci
   

   Use

   Plant expression

   Mutation

   BsaI/BbsI sites removed by point-mutation

   Available Since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJOG417

   Plasmid

   #105341

   Purpose

   Yeast two hybrid

   Depositor

   Johannes Stuttmann

   Article

   Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   Aug. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJOG418

   Plasmid

   #105342

   Purpose

   Yeast two hybrid

   Depositor

   Johannes Stuttmann

   Article

   Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   Aug. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMINTC3H

   Plasmid

   #110075

   Purpose

   Analogous to pMINTNH3 but with a C-terminal 3C cleavage site and His10 tag.

   Depositor

   Markus Seeger

   Article

   Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

   Type

   Empty backbone

   Tags

   3C cleavage site - His10 Tag

   Expression

   Bacterial

   Available Since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFH75

   Plasmid

   #128224

   Purpose

   classic sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU6 promoter module (pICSL90003)

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   classic sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU6 promoter module (pICSL90003)
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMINTC3GH (Hyg)

   Plasmid

   #110077

   Purpose

   Analogous to pMINTNH3 but with a C-terminal 3C cleavage site and a GFP-His10 tag fusion.

   Depositor

   Markus Seeger

   Article

   Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

   Type

   Empty backbone

   Tags

   3C cleavage site - GFP - His10 Tag

   Expression

   Bacterial

   Available Since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMC-XI2

   Plasmid

   #176156

   Purpose

   preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XI-2

   Depositor

   Florian David

   Article

   Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   Feb. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMEXC3GH (Hyg)

   Plasmid

   #110082

   Purpose

   Analogous to pMINTC3GH but containing oriM for episomal replication in mycobacteria.

   Depositor

   Markus Seeger

   Article

   Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

   Type

   Empty backbone

   Tags

   3C cleavage site - GFP - His10 Tag

   Expression

   Bacterial

   Available Since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJRA99

    Plasmid

    #209334

    Purpose

    Gateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of untagged protein.

    Depositor

    Lucia Strader

    Article

    Allen et al BMC Res Notes. 2025 Apr 8;18(1):149. doi

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pf CAG mc2 IRES blast

    Plasmid

    #206235

    Purpose

    For expressing cloned exons as circular RNA.

    Depositor

    Simon Conn, Brett Stringer

    Article

    Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

    Type

    Empty backbone

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Sept. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pf CAG mc2 IRES puro

    Plasmid

    #206233

    Purpose

    For expressing cloned exons as circular RNA.

    Depositor

    Simon Conn, Brett Stringer

    Article

    Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

    Type

    Empty backbone

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Sept. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMEXC3GH (Kan)

    Plasmid

    #110081

    Purpose

    Analogous to pMINTC3GH but containing oriM for episomal replication in mycobacteria.

    Depositor

    Markus Seeger

    Article

    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

    Type

    Empty backbone

    Tags

    3C cleavage site - GFP - His10 Tag

    Expression

    Bacterial

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRS423-TyrSgH

    Plasmid

    #163973

    Purpose

    Helper plasmid for cloning gRNAs under the control of the tRNA(Tyr) promoter

    Depositor

    Jiazhang Lian

    Article

    Dong et al ACS Synth Biol. 2020 Sep 18;9(9):2252-22

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Yeast

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSB700 lenti gRNA KanR neo

    Plasmid

    #167909

    Purpose

    Lentiviral vector for expressing U6 sgRNA cloning plasmid

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Available Since

    Feb. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMINTC3GH (Kan)

    Plasmid

    #110076

    Purpose

    Analogous to pMINTNH3 but with a C-terminal 3C cleavage site and a GFP-His10 tag fusion.

    Depositor

    Markus Seeger

    Article

    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

    Type

    Empty backbone

    Tags

    3C cleavage site - GFP - His10 Tag

    Expression

    Bacterial

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMAD-Gate2

    Plasmid

    #25941

    Depositor

    Kamil Onder

    Article

    Maier et al Assay Drug Dev Technol. 2010 Oct;8(5):62

    Insert

    
    

    Tags

    VP16 Fusion Protein

    Expression

    Mammalian

    Available Since

    Aug. 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJRA-GW

    Plasmid

    #209333

    Purpose

    Gateway cloning compatible binary vector for agrobacterium mediated transformation. MCS upstream of gateway.

    Depositor

    Lucia Strader

    Article

    Allen et al BMC Res Notes. 2025 Apr 8;18(1):149. doi

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. plIVMD156

    Plasmid

    #160934

    Purpose

    In vivo mRNA display Gateway Destination construct

    Depositor

    Saeed Tavazoie

    Article

    Oikonomou et al Proc Natl Acad Sci U S A. 2020 Oct 9. pi

    Insert

    MCP-Gateway cloning site-HIS tag, 3'UTR MS2 Stem Loop
    

    Tags

    HIS tag, 3'UTR MS2 Stem Loop

    Expression

    Yeast

    Promoter

    MET25

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pM115: CasRx control presgRNA

    Plasmid

    #166867

    Purpose

    U6-driven expression of control (non-targeting) presgRNA compatible with CasRx.

    Depositor

    Guei-Sheung Liu

    Article

    Chuang et al Front Cell Dev Biol. 2021 Jun 11;9:66787

    Insert

    non-targeting presgRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    July 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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