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pDONR223_PEX3_WT

Plasmid

#81779

Purpose

Gateway Donor vector containing PEX3 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PEX3 (PEX3 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_TRAF3_WT

Plasmid

#81755

Purpose

Gateway Donor vector containing TRAF3 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

TRAF3 (TRAF3 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PTEN_WT

Plasmid

#81737

Purpose

Gateway Donor vector containing PTEN , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PTEN (PTEN Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PIK3CB_WT

Plasmid

#82221

Purpose

Gateway Donor vector containing PIK3CB , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PIK3CB (PIK3CB Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_IRS1_WT

Plasmid

#82214

Purpose

Gateway Donor vector containing IRS1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

IRS1 (IRS1 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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CaSpeR dhd 3X FLAG

Plasmid

#113002

Purpose

P element vector for transposon insertion of Flag-tagged Drosophila dhd into Drosophila genome

Depositor

Terry Orr-Weaver

Insert

dhd (dhd Fly)

Use

P insertion vector

Tags

FLAG

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pDC32

Plasmid

#171204

Purpose

bacterial expression for HIS purification of aa1-698 of MEG-3

Depositor

Geraldine Seydoux

Article

Schmidt et al bioRxiv 2020.10.15.340570

Insert

meg-3 (meg-3 Nematode)

Tags

6xHIS

Expression

Bacterial

Mutation

aa1-698

Available Since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pAAP20

Plasmid

#171206

Purpose

bacterial expression for HIS purification of the MEG-3 C-terminus

Depositor

Geraldine Seydoux

Article

Schmidt et al bioRxiv 2020.10.15.340570

Insert

meg-3 (meg-3 Nematode)

Tags

8xHIS

Expression

Bacterial

Mutation

aa545-862

Available Since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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Zeta-globin

Plasmid

#19262

Depositor

Philip Leder

Article

Leder et al Nature. . 293(5829):196-200.

Insert

zeta globin (Hba-x Mouse)

Expression

Bacterial

Available Since

Sept. 22, 2008

Availability

Academic Institutions and Nonprofits only
pE-35S-miniSdd7-nCas9-PBE-GmPPO2

Plasmid

#204855

Purpose

For cytosine base editing using miniSdd7 at PPO2 site in soybean (Agrobacterium-mediated transformation)

Depositor

Caixia Gao

Article

Huang et al Cell. 2023 Jul 20;186(15):3182-3195.e14.

Insert

miniSdd7-nSpCas9(D10A)-UGI-mScarlet

Use

CRISPR

Expression

Plant

Mutation

D10A in SpCas9

Promoter

35S, AtU6-26p

Available Since

Aug. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pKD001 pME minprom_EGFP

Plasmid

#195955

Purpose

Gateway pME fluorophore

Depositor

Christian Mosimann

Article

Prummel et al Nat Commun. 2019 Aug 26;10(1):3857. doi:

Insert

mouse beta-globin minimal promoter EGFP

Use

Other

Available Since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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CABE-T3.155

Plasmid

#193270

Purpose

Expression of CABE-T3.155 base editor in mammalian cells, CMV promoter

Depositor

Nicole Gaudelli

Article

Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

Insert

CABE-T3.155

Expression

Mammalian

Mutation

D10A (Cas9), TadA mutations within

Promoter

CMV

Available Since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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CBE-T1.14

Plasmid

#193277

Purpose

Expression of CBE-T1.14 base editor in mammalian cells, CMV promoter

Depositor

Nicole Gaudelli

Article

Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

Insert

CBE-T1.14

Expression

Mammalian

Mutation

D10A (Cas9), TadA mutations within

Promoter

CMV

Available Since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-DIO-GRAB_OT1.0

Plasmid

#185387

Purpose

Expresses the genetically-encoded fluorescent oxytocin(OT) sensor GRAB_OT1.0 in a cre-dependent manner

Depositor

Yulong Li

Article

Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

GPCR activation based oxytocin sensor GRAB_OT1.0

Use

AAV

Promoter

EFS

Available Since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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EF1a_KLF15_P2A_Hygro_Barcode

Plasmid

#120501

Purpose

Barcoded lentiviral vector to express KLF15 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

KLF15 (KLF15 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a_KLF5_P2A_Hygro_Barcode

Plasmid

#120491

Purpose

Barcoded lentiviral vector to express KLF5 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

KLF5 (KLF5 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q30

Plasmid

#111744

Purpose

Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

Depositor

Cheryl Arrowsmith

Article

Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

Insert

HTT (HTT Human)

Use

Baculovirus expression

Tags

FLAG

Expression

Mammalian

Mutation

Q30 (polyQ repeat)

Promoter

CMV and P10

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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ACO2_pLX307

Plasmid

#98312

Purpose

Lentiviral expression of ACO2

Depositor

William Hahn, Sefi Rosenbluh

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

ACO2 (ACO2 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

E1Fa

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (AAV2)

Viral Prep

#112677-AAV2

Purpose

Ready-to-use AAV2 particles produced from pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (#112677). In addition to the viral particles, you will also receive purified pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP plasmid DNA. EF1a-driven, Cre-dependent color switch. This AAV directs expression of nuclear mCherry in the absence of Cre. In the presence of Cre, nuclear EGFP (and not mCherry) will be expressed. These AAV preparations are suitable purity for injection into animals.

Depositor

Brandon Harvey

Article

Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

Promoter

EF1a

Tags

mCherry (Cre-negative cells), EGFP (Cre-positive cells)

Available Since

Jan. 17, 2020

Availability

Academic Institutions and Nonprofits only
pG6(3'Pro) (P#1478)

Plasmid

#14660

Depositor

Matija Peterlin

Article

Taube et al Mol Cell Biol. 2002 Jan . 22(1):321-31.

Insert

UAS repeats

Expression

Bacterial

Available Since

June 30, 2008

Availability

Academic Institutions and Nonprofits only