We narrowed to 15,187 results for: pET

1. pJMP8930

   Plasmid

   #220905

   Purpose

   MobileCRiSPRi system encoded on a kanR Tn7 transposon; PD-sgRNA (BsaI site) and PC-dCas9-novo (for cloning new guides)

   Depositor

   Jason Peters

   Article

   Hall et al Appl Environ Microbiol. 2024 Sep 26:e003

   Insert

   dCas9-novo
   

   Use

   CRISPR

   Tags

   3xMyc

   Expression

   Bacterial

   Mutation

   D10A, H840A

   Available Since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. Tol2-cmlc2-Trpa1b-2A-EGFP

   Plasmid

   #106405

   Purpose

   For expressing Trpa1b-2A-EGFP in zebrafish cardiomyocytes.

   Depositor

   Randall Peterson

   Article

   Lam et al Sci Rep. 2017 Sep 19;7(1):11839. doi: 10

   Inserts

   cmlc2 promoter
   Trpa1b (trpa1b Zebrafish)
   

   Use

   Gateway

   Tags

   2A-EGFP

   Available Since

   May 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pECFP-Rac1Q61L

   Plasmid

   #105292

   Purpose

   Constitutively active Rac 1

   Depositor

   Kenneth Yamada

   Article

   Petrie et al J Cell Biol. 2012 Apr 30;197(3):439-55.

   Insert

   Rac1 (RAC1 Human)
   

   Tags

   CFP

   Expression

   Mammalian

   Mutation

   Q61L

   Available Since

   Feb. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-3xhfYFP

   Plasmid

   #186544

   Purpose

   Hyperfolder YFP, triple fusion: cytosolic expression in mammalian cells

   Depositor

   Gregory Petsko

   Article

   Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

   Insert

   Hyperfolder YFP (3X)
   

   Expression

   Mammalian

   Mutation

   mGreenLantern-F46L/C48S/V68L/A69M/C70V/K101E/T105…

   Promoter

   CMV

   Available Since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. Mito-LSSmGFP

   Plasmid

   #186536

   Purpose

   Localization of LSSmGFP fluorescent protein (ex/em = 400/510 nm) to mitochondria

   Depositor

   Gregory Petsko

   Article

   Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

   Insert

   LSSmGFP
   

   Tags

   Human COX8a first 29 amino acids

   Expression

   Mammalian

   Mutation

   hfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206K

   Promoter

   CMV

   Available Since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. ER2-mGreenLantern

   Plasmid

   #186548

   Purpose

   Localization and retention of mGreenLantern fluorescent protein in the ER; improved targeting efficiency

   Depositor

   Gregory Petsko

   Article

   Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

   Insert

   mGreenLantern
   

   Tags

   Calreticulin and KDEL

   Expression

   Mammalian

   Mutation

   Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

   Promoter

   CMV

   Available Since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. ER2-LSSmGFP

   Plasmid

   #186538

   Purpose

   Localization and retention of LSSmGFP fluorescent protein (ex/em = 400/510 nm) in the ER; improved targeting efficiency

   Depositor

   Gregory Petsko

   Article

   Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

   Insert

   LSSmGFP
   

   Tags

   Calreticulin and KDEL

   Expression

   Mammalian

   Mutation

   hfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206K

   Promoter

   CMV

   Available Since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. ER2-hfYFP

   Plasmid

   #186518

   Purpose

   Localization and retention of hyperfolder YFP fluorescent protein in the ER; improved targeting efficiency

   Depositor

   Gregory Petsko

   Article

   Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

   Insert

   Hyperfolder YFP
   

   Tags

   Calreticulin and KDEL

   Expression

   Mammalian

   Mutation

   mGreenLantern-F46L/C48S/V68L/A69M/C70V/K101E/T105…

   Promoter

   CMV

   Available Since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV Flag cycT2b (P#1718)

   Plasmid

   #14599

   Depositor

   Matija Peterlin

   Article

   Kurosu et al Gene. 2004 Dec 8. 343(1):173-9.

   Insert

   cycT2 (CCNT2 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   A137T compared to NP_490595.1

   Available Since

   June 30, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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10. pECFP-Cdc42Q61L

    Plasmid

    #105294

    Purpose

    Constitutively active Cdc42

    Depositor

    Kenneth Yamada

    Article

    Petrie et al J Cell Biol. 2012 Apr 30;197(3):439-55.

    Insert

    Cdc42 (CDC42 Human)
    

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Q61L

    Available Since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEF NEF NBP1 (P#375)

    Plasmid

    #14657

    Depositor

    Matija Peterlin

    Article

    Lu et al Immunity. 1998 May . 8(5):647-56.

    Insert

    NEF NBP1 (ATP6V1H Human)
    

    Expression

    Mammalian

    Available Since

    June 30, 2008

    Availability

    Academic Institutions and Nonprofits only

12. pCytERM-LSSmGFP

    Plasmid

    #186540

    Purpose

    Visualization of the ER in cells using LSSmGFP (ex/em = 400/510 nm); compatible with OSER assay.

    Depositor

    Gregory Petsko

    Article

    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

    Insert

    LSSmGFP
    

    Tags

    Cytochrome p450 first 29 animo acids (Oryctolagus…

    Expression

    Mammalian

    Mutation

    hfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206K

    Promoter

    CMV

    Available Since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. SRS-MTBD(22/19), AB(monomer), wt

    Plasmid

    #22380

    Depositor

    Ron Vale

    Article

    Gibbons et al J Biol Chem. 2005 Jun 24. 280(25):23960-

    Insert

    Seryl tRNA synthetase (SRS) – mouse cytoplasmic dynein microtubule binding domain (MTBD) fusion protein
    

    Tags

    His and myc

    Expression

    Bacterial

    Available Since

    Nov. 24, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-Trpa1b-FLAG

    Plasmid

    #106403

    Purpose

    Mammalian expression vector containing zebrafish Trpa1b with C-terminal FLAG tag.

    Depositor

    Randall Peterson

    Article

    Lam et al Sci Rep. 2017 Sep 19;7(1):11839. doi: 10

    Insert

    Trpa1b-FLAG (trpa1b Zebrafish)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    June 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. SRS-MTBD(26/19) AB(monomer), wt

    Plasmid

    #22381

    Depositor

    Ron Vale

    Article

    Gibbons et al J Biol Chem. 2005 Jun 24. 280(25):23960-

    Insert

    Seryl tRNA synthetase (SRS) – mouse cytoplasmic dynein microtubule binding domain (MTBD) fusion protein
    

    Tags

    myc

    Expression

    Bacterial

    Available Since

    Nov. 24, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. pOPO640

    Plasmid

    #195296

    Purpose

    pTA106-Myacmini-KanR, donor plasmid with mini-transposon of McCAST (Tn7575).

    Depositor

    Joseph Peters

    Article

    Hsieh et al Nucleic Acids Res. 2023 Jan 25;51(2):765

    Insert

    miniMcCAST (Tn7575) with KanR
    

    Expression

    Bacterial

    Available Since

    March 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUC57-Kan_AVR-Pia

    Plasmid

    #123653

    Purpose

    Level 0 Golden-Gate compatible vector

    Depositor

    Sophien Kamoun

    Article

    Petit-Houdenot et al Mol Plant Microbe Interact. 2020 Aug;33(

    Insert

    AVR-Pia
    

    Use

    Golden gate

    Available Since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUC57-Kan_TH16_AVR-Pib_100

    Plasmid

    #121378

    Purpose

    Level 0 Golden-Gate compatible vector

    Depositor

    Sophien Kamoun

    Article

    Petit-Houdenot et al Mol Plant Microbe Interact. 2020 Aug;33(

    Insert

    TH16_AVR-Pib_100
    

    Use

    Golden gate

    Mutation

    Magnaporthe oryzae

    Available Since

    March 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUC57-Kan_TH12-rn_00121641

    Plasmid

    #121371

    Purpose

    Level 0 Golden-Gate compatible vector

    Depositor

    Sophien Kamoun

    Article

    Petit-Houdenot et al Mol Plant Microbe Interact. 2020 Aug;33(

    Insert

    TH12-rn_00121641
    

    Use

    Golden gate

    Mutation

    Magnaporthe oryzae

    Available Since

    March 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUC57-Kan_PH14-rn_00186401

    Plasmid

    #121369

    Purpose

    Level 0 Golden-Gate compatible vector

    Depositor

    Sophien Kamoun

    Article

    Petit-Houdenot et al Mol Plant Microbe Interact. 2020 Aug;33(

    Insert

    PH14-rn_00186401
    

    Use

    Golden gate

    Mutation

    Magnaporthe oryzae

    Available Since

    March 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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