We narrowed to 19,051 results for: Tec;

1. shCDK4/6(1)

   Plasmid

   #73554

   Purpose

   vector encoding both shRNA against CDK4(1) and shRNA against CDK6(1)

   Depositor

   Gerardo Ferbeyre

   Article

   Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d

   Insert

   shRNA against CDK4 + shRNA against CDK6
   

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   LTR

   Available Since

   Aug. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

2. AA011

   Plasmid

   #215973

   Purpose

   Fragmid fragment: (N' terminus) for C>T base editing

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   Start; BPNLS_v1.1; rAPOBEC1_v1.1; Linker_v1.1
   

   Use

   Fragment

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. AA010

   Plasmid

   #216038

   Purpose

   Fragmid fragment: (C' terminus) for C>T base editing

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   UGI_v1.1; NpNLS_v1.1
   

   Use

   Fragment

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. AA013

   Plasmid

   #215975

   Purpose

   Fragmid fragment: (N' terminus) for A>G base editing

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   Start; BPNLS_v1.1; ABE8e_v1.1; Linker_v1.1
   

   Use

   Fragment

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBEAST-J23101-sfGFP

   Plasmid

   #116917

   Purpose

   Strong constitutive expression of super-folder GFP for cell-free expression

   Depositor

   Jerome Bonnet

   Article

   Voyvodic et al Nat Commun. 2019 Apr 12;10(1):1697. doi:

   Insert

   sfGFP
   

   Use

   Synthetic Biology; Cell-free protein synthesis

   Promoter

   J23101

   Available Since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. MMLV-RT for Split-PE (pEH45)

   Plasmid

   #190105

   Purpose

   Expresses MMLV-RT (full-length, from PE2) for Split-PE use

   Depositor

   Keith Joung

   Article

   Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

   Insert

   MMLV-RT
   

   Tags

   bpNLS

   Expression

   Mammalian

   Mutation

   RT pentamutant from PE2 (D200N, T306K, W313F, T33…

   Promoter

   CMV

   Available Since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. EPB104

   Plasmid

   #68369

   Purpose

   contains human U6 promoter

   Depositor

   Eric-Paul Bennett

   Article

   Lonowski et al Nat Protoc. 2017 Mar;12(3):581-603. doi:

   Insert

   U6 promoter (RNU6-1 Human)
   

   Expression

   Bacterial

   Promoter

   U6

   Available Since

   Feb. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. EE-BE4max

   Plasmid

   #138158

   Purpose

   C-to-T base editor

   Depositor

   David Liu

   Article

   Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

   Insert

   rAPOBEC1(EE)-nSpCas9-UGI-UGI
   

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Within rAPOBEC1 R126E + R132E; within Cas9 D10A

   Promoter

   CMV

   Available Since

   Feb. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pIW447-CRISPR XYL2

   Plasmid

   #98906

   Purpose

   K. marxianus CRISPR Plasmid for XYL2 editing

   Depositor

   Ian Wheeldon

   Article

   Lobs et al Biotechnol Biofuels. 2017 Jun 24;10:164.

   Inserts

   Codon optimized Cas9
   sgRNA expression cassette
   

   Use

   CRISPR and Synthetic Biology

   Tags

   SV40

   Expression

   Yeast

   Available Since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGWB420

    Plasmid

    #74814

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with 10xMyc (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Promoter

    CaMV35S

    Available Since

    July 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGWB508

    Plasmid

    #74850

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with 6xHis (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Promoter

    CaMV35S

    Available Since

    Oct. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. EK0294 SFFV-mCherry-SG(s70587-W)SG-EGFP (FLP-IN)

    Plasmid

    #191141

    Purpose

    Positive control for Sensor 1 (EK0285) where the TAG stop codon is replaced with TGG tryptophan codon

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    mCherry:s70587-W:EGFP
    

    Expression

    Mammalian

    Mutation

    UAG>UGG (stop>Trp)

    Promoter

    SFFV

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMU-4

    Plasmid

    #172353

    Purpose

    CRISPR gene editing in Streptomyces, codon-optimized Cas9 behind theophylline riboswitch and D4 promoter, with custom gRNA

    Depositor

    Eriko Takano

    Article

    Ye et al J Ind Microbiol Biotechnol. 2020 May;47(

    Insert

    D4-TheoR-cas9
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    D4

    Available Since

    Dec. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCH62 (denAsCas12a-KRAB piggyBac)

    Plasmid

    #217333

    Purpose

    piggyBac expression of denAsCas12a-KRAB

    Depositor

    Luke Gilbert

    Article

    Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

    Insert

    denAsCas12a
    

    Use

    CRISPR

    Tags

    6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2

    Expression

    Mammalian

    Mutation

    D908A/E174R/S542R/K548R

    Promoter

    CAG

    Available Since

    March 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-CaMKII-GRAB_eCB2.0

    Plasmid

    #164608

    Purpose

    Express the endocannabinoid sensor GRAB_eCB2.0 in CaMKII positive neurons

    Depositor

    Yulong Li

    Article

    Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

    Insert

    Endocannabinoid sensor GRAB_eCB2.0
    

    Use

    AAV

    Mutation

    S383T

    Promoter

    CaMKII

    Available Since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBK-YE2-BE3

    Plasmid

    #85176

    Purpose

    Expresses YE2-BE3 in mammalian cells

    Depositor

    David Liu

    Article

    Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

    Insert

    YEE-BE3
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. PDGFR-TMD (MTK 3B) (pZYW025)

    Plasmid

    #194218

    Purpose

    Mammalian Toolkit part 3b encoding the transmembrane domain of PDGFR

    Depositor

    Hana El-Samad

    Article

    Weinberg et al bioRxiv 2021.04.20.440678

    Insert

    PDGFR TMD
    

    Use

    Synthetic Biology

    Promoter

    None

    Available Since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. T2A-mCherry (MTK 4a) (pAN3844)

    Plasmid

    #194223

    Purpose

    Mammalian Toolkit part 4a encoding a T2A polycistronic element followed by mCherry

    Depositor

    Hana El-Samad

    Article

    Weinberg et al bioRxiv 2021.04.20.440678

    Insert

    T2A-mCherry
    

    Use

    Synthetic Biology

    Promoter

    None

    Available Since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. PGK-LCB1_v1.3-NotchGal4VP64-T2A-mCherry (pZYW046)

    Plasmid

    #194229

    Purpose

    Lentiviral expression vector expressing LCB1 SynNotch and mCherry transduction marker

    Depositor

    Hana El-Samad

    Article

    Weinberg et al bioRxiv 2021.04.20.440678

    Insert

    PGK-LCB1-SynNotch-T2A-mCherry
    

    Use

    Lentiviral

    Promoter

    PGK

    Available Since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. TALEN1257

    Plasmid

    #32280

    Depositor

    Keith Joung

    Article

    Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

    Insert

    TAL array targeting hey2 (hey2 Zebrafish)
    

    Use

    T7

    Tags

    3X FLAG and WT FOKI

    Expression

    Mammalian

    Available Since

    Oct. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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