We narrowed to 37,775 results for: Mut

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pCEP4-CCR5(R5gp120-Hi)

Plasmid

#98954

Purpose

Mammalian expression plasmid for myc-tagged human CCR5 mutant R5gp120-Hi

Depositor

Erik Procko

Article

Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

Insert

CCR5 (CCR5 Human)

Tags

Signal/leader sequence from Influenza A virus HA …

Expression

Mammalian

Mutation

R5gp120-Hi variant: P8L, I9E, I12M, S17E, P19D, Q…

Promoter

CMV

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pCEP4-CCR5(R5gp120-Hi)

Plasmid

#98954

Purpose

Mammalian expression plasmid for myc-tagged human CCR5 mutant R5gp120-Hi

Depositor

Erik Procko

Article

Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

Insert

CCR5 (CCR5 Human)

Tags

Signal/leader sequence from Influenza A virus HA …

Expression

Mammalian

Mutation

R5gp120-Hi variant: P8L, I9E, I12M, S17E, P19D, Q…

Promoter

CMV

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5 TO FRT Myc ArB K106R

Plasmid

#59807

Purpose

Allows the integration of Myc ArB K106R in the genome and Tet-inducible expression

Depositor

Stephen Taylor

Article

Girdler et al J Cell Sci. 2006 Sep 1;119(Pt 17):3664-7

Insert

Aurora B (AURKB Human)

Tags

Myc

Expression

Mammalian

Mutation

K106R, kinase mutant

Promoter

hybrid human cytomegalovirus (CMV)/TetO2 promoter

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA5 TO FRT Myc ArB K106R

Plasmid

#59807

Purpose

Allows the integration of Myc ArB K106R in the genome and Tet-inducible expression

Depositor

Stephen Taylor

Article

Girdler et al J Cell Sci. 2006 Sep 1;119(Pt 17):3664-7

Insert

Aurora B (AURKB Human)

Tags

Myc

Expression

Mammalian

Mutation

K106R, kinase mutant

Promoter

hybrid human cytomegalovirus (CMV)/TetO2 promoter

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only

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HNF4A_P2-285/-192

Plasmid

#31053

Depositor

Gerhart Ryffel

Article

Wirsing et al Diabet Med. 2010 Jun . 27(6):631-5.

Insert

HNF4A P2 promoter (HNF4A Human)

Use

Luciferase

Expression

Mammalian

Mutation

-285 to -1 of P2 HNF4A promoter with mutation -19…

Available Since

Oct. 6, 2011

Availability

Academic Institutions and Nonprofits only

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HNF4A_P2-285/-192

Plasmid

#31053

Depositor

Gerhart Ryffel

Article

Wirsing et al Diabet Med. 2010 Jun . 27(6):631-5.

Insert

HNF4A P2 promoter (HNF4A Human)

Use

Luciferase

Expression

Mammalian

Mutation

-285 to -1 of P2 HNF4A promoter with mutation -19…

Available Since

Oct. 6, 2011

Availability

Academic Institutions and Nonprofits only

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HNF4A_P2-285/-169

Plasmid

#31052

Depositor

Gerhart Ryffel

Article

Wirsing et al Diabet Med. 2010 Jun . 27(6):631-5.

Insert

HNF4A P2 promoter (HNF4A Human)

Use

Luciferase

Expression

Mammalian

Mutation

-285 to -1 of P2 HNF4A promoter with mutation -16…

Available Since

Oct. 6, 2011

Availability

Academic Institutions and Nonprofits only

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HNF4A_P2-285/-169

Plasmid

#31052

Depositor

Gerhart Ryffel

Article

Wirsing et al Diabet Med. 2010 Jun . 27(6):631-5.

Insert

HNF4A P2 promoter (HNF4A Human)

Use

Luciferase

Expression

Mammalian

Mutation

-285 to -1 of P2 HNF4A promoter with mutation -16…

Available Since

Oct. 6, 2011

Availability

Academic Institutions and Nonprofits only

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HNF4A_P2-285/-136

Plasmid

#31051

Depositor

Gerhart Ryffel

Article

Wirsing et al Diabet Med. 2010 Jun . 27(6):631-5.

Insert

HNF4A P2 promoter (HNF4A Human)

Use

Luciferase

Expression

Mammalian

Mutation

-285 to -1 of P2 HNF4A promoter with mutation -13…

Available Since

Oct. 6, 2011

Availability

Academic Institutions and Nonprofits only

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HNF4A_P2-285/-136

Plasmid

#31051

Depositor

Gerhart Ryffel

Article

Wirsing et al Diabet Med. 2010 Jun . 27(6):631-5.

Insert

HNF4A P2 promoter (HNF4A Human)

Use

Luciferase

Expression

Mammalian

Mutation

-285 to -1 of P2 HNF4A promoter with mutation -13…

Available Since

Oct. 6, 2011

Availability

Academic Institutions and Nonprofits only

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pD10AH840AhCas9 (GB1041)

Plasmid

#68207

Purpose

Provides the human codon optimized CDS of Cas9 protein with mutated (D10A, H840A) and inactivated catalytic domains as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

Cas9 coding region with mutated (D10A, H840A) and inactivated catalytic domains (human codon optimised)

Use

CRISPR and Synthetic Biology

Mutation

BsaI and BsmBI sites removed; human codon optimis…

Available Since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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pD10AH840AhCas9 (GB1041)

Plasmid

#68207

Purpose

Provides the human codon optimized CDS of Cas9 protein with mutated (D10A, H840A) and inactivated catalytic domains as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

Cas9 coding region with mutated (D10A, H840A) and inactivated catalytic domains (human codon optimised)

Use

CRISPR and Synthetic Biology

Mutation

BsaI and BsmBI sites removed; human codon optimis…

Available Since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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Tau BA 12->10 N296N

Plasmid

#203383

Purpose

For transfection and in vitro assay

Depositor

Stefan Stamm

Article

Margvelani et al Biochim Biophys Acta Mol Basis Dis. 2024

Insert

MAPT (MAPT Human)

Expression

Mammalian

Mutation

N296N (silent mutation)

Promoter

CMVf

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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Tau BA 12->10 N296N

Plasmid

#203383

Purpose

For transfection and in vitro assay

Depositor

Stefan Stamm

Article

Margvelani et al Biochim Biophys Acta Mol Basis Dis. 2024

Insert

MAPT (MAPT Human)

Expression

Mammalian

Mutation

N296N (silent mutation)

Promoter

CMVf

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pdnCas3-CDA1

Plasmid

#229535

Purpose

pMV_hyg encoding dnCas3(H74A+D75A)-CDA1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning

Depositor

Kevin Verstrepen

Article

Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:

Inserts

dnCas3
Cytidine deaminase
Uracil glycosylase inhibitor

Tags

XTEN

Expression

Bacterial and Yeast

Mutation

codon optimized for S.cerevisiae and codon optimi…

Available Since

Jan. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pdnCas3-CDA1

Plasmid

#229535

Purpose

pMV_hyg encoding dnCas3(H74A+D75A)-CDA1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning

Depositor

Kevin Verstrepen

Article

Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:

Inserts

dnCas3
Cytidine deaminase
Uracil glycosylase inhibitor

Tags

XTEN

Expression

Bacterial and Yeast

Mutation

codon optimized for S.cerevisiae and codon optimi…

Available Since

Jan. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pdnCas3-APOBEC1

Plasmid

#229537

Purpose

pMV_hyg encoding dnCas3(H74A+D75A)-rAPOBEC1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning

Depositor

Kevin Verstrepen

Article

Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:

Inserts

rAPOBEC1
dnCas3
Uracil glycosylase inhibitor

Tags

XTEN

Expression

Bacterial and Yeast

Mutation

codon optimized for S.cerevisiae and codon optimi…

Available Since

Jan. 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pdnCas3-APOBEC1

Plasmid

#229537

Purpose

pMV_hyg encoding dnCas3(H74A+D75A)-rAPOBEC1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning

Depositor

Kevin Verstrepen

Article

Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:

Inserts

rAPOBEC1
dnCas3
Uracil glycosylase inhibitor

Tags

XTEN

Expression

Bacterial and Yeast

Mutation

codon optimized for S.cerevisiae and codon optimi…

Available Since

Jan. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pTFG027_Spike_sACE2-resistant_D614G_F486S_3xFlag

Plasmid

#191572

Purpose

Mutated SARS-CoV-2 Spike under CMV with D614G, C-term 19 amino acid truncation, and 3x FLAG. Encodes F486S mutation. For expression and pseudotyping lentiviral particles.

Depositor

Joshua Leonard

Article

Gunnels et al Small. 2022 May;18(19):e2200125. doi: 10

Insert

SARS-CoV-2 Spike Protein (S Synthetic)

Tags

3x FLAG

Expression

Mammalian

Mutation

D614G and F486S (confers reduced binding affinity…

Promoter

CMV

Available Since

Aug. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pTFG027_Spike_sACE2-resistant_D614G_F486S_3xFlag

Plasmid

#191572

Purpose

Mutated SARS-CoV-2 Spike under CMV with D614G, C-term 19 amino acid truncation, and 3x FLAG. Encodes F486S mutation. For expression and pseudotyping lentiviral particles.

Depositor

Joshua Leonard

Article

Gunnels et al Small. 2022 May;18(19):e2200125. doi: 10

Insert

SARS-CoV-2 Spike Protein (S Synthetic)

Tags

3x FLAG

Expression

Mammalian

Mutation

D614G and F486S (confers reduced binding affinity…

Promoter

CMV

Available Since

Aug. 9, 2024

Availability

Academic Institutions and Nonprofits only

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