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pLXSN-H-Ras_V12-S35

Plasmid

#39517

Depositor

Julian Downward

Article

Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

Insert

HRAS V12 S35 (HRAS Human)

Use

Retroviral

Expression

Mammalian

Mutation

G12V and T35S

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only
pmEos2-Linker_PCNA-MutC

Plasmid

#98272

Purpose

for low level expression of mEos2-PCNA in mammalian cells

Depositor

Mike Heilemann

Article

Zessin et al Sci Rep. 2016 Jan 13;6:18779. doi: 10.10

Insert

PCNA (PCNA Human)

Tags

mEos2

Expression

Mammalian

Mutation

Silent mutated Sequence: GACGCCGTAGTTATATCTTGC (O…

Promoter

CMV

Available Since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

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pLXSN-H-Ras_V12-E38

Plasmid

#39520

Depositor

Julian Downward

Article

Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

Insert

HRAS V12 E38 (HRAS Human)

Use

Retroviral

Expression

Mammalian

Mutation

G12V and D38E

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only
pMGF170

Plasmid

#96997

Purpose

ish1-mCherry insertion cassette with NAT resistance gene

Depositor

Adam Frost, Wesley Sundquist

Article

Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

Insert

ish1 (ish1 Fission Yeast)

Tags

GST and mCherry

Expression

Bacterial

Mutation

ish1 C-terminal domain fused with mCherry + NAT r…

Promoter

N/A

Available Since

Aug. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pBABE-puroGFP-Eps8WT

Plasmid

#74956

Purpose

mammalian expression of Eps8 fused to GFP

Depositor

Giorgio Scita

Article

Frittoli et al Immunity. 2011 Sep 23;35(3):388-99. doi:

Insert

Eps8 (Eps8 Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

May 3, 2016

Availability

Academic Institutions and Nonprofits only
mTFP1-ELP-1-25

Plasmid

#55478

Purpose

Localization: Golgi/ER, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

ELP (KDELR2 Human)

Tags

mTFP1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-H-Ras_V12-S35

Plasmid

#39505

Depositor

Julian Downward

Article

Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

Insert

HRAS V12 S35 (HRAS Human)

Expression

Mammalian

Mutation

G12V and T35S

Promoter

CMV

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3-H-Ras_V12-G37

Plasmid

#39506

Depositor

Julian Downward

Article

Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

Insert

HRAS V12 G37 (HRAS Human)

Expression

Mammalian

Mutation

G12V and E37G

Promoter

CMV

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3-H-Ras_V12-C40

Plasmid

#39508

Depositor

Julian Downward

Article

Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

Insert

HRAS V12 C40 (HRAS Human)

Expression

Mammalian

Mutation

G12V and Y40C

Promoter

CMV

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only
pLXSN-H-Ras_V12-G37

Plasmid

#39518

Depositor

Julian Downward

Article

Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

Insert

HRAS V12 G37 (HRAS Human)

Use

Retroviral

Expression

Mammalian

Mutation

G12V and E37G

Available Since

Aug. 30, 2012

Availability

Academic Institutions and Nonprofits only
pLXSN-H-Ras_V12-C40

Plasmid

#39521

Depositor

Julian Downward

Article

Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

Insert

HRAS V12 C40 (HRAS Human)

Use

Retroviral

Expression

Mammalian

Mutation

G12V and Y40C

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only
pLXSN-H-Ras_V12-A38

Plasmid

#39519

Depositor

Julian Downward

Article

Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

Insert

HRAS V12 A38 (HRAS Human)

Use

Retroviral

Expression

Mammalian

Mutation

G12V and D38A

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only
Lyn-FRB_star-P2A-mVenus-FKBP-5ptase

Plasmid

#241305

Purpose

Mammalian expression of Lyn-FRB_star and mVenus-FKBP-5ptase of INPP5E

Depositor

Justin Taraska

Article

Obashi et al Structure. Volume 34, Issue 1, 8 January

Insert

INPP5E (INPP5E Human)

Tags

mVenus, FKBP

Expression

Mammalian

Mutation

214-644 aa

Promoter

CMV

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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mVenus-synaptojanin1(498-901)-CAAX

Plasmid

#241303

Purpose

Mammalian expression of truncated form of synaptojanin-1 with CAAX motif fused to mVenus

Depositor

Justin Taraska

Article

Obashi et al Structure. Volume 34, Issue 1, 8 January

Insert

Synaptojanin-1 (SYNJ1 Human)

Tags

mVenus

Expression

Mammalian

Mutation

498-901 aa

Promoter

CMV

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pANAP-P2A-eRF(E55D)

Plasmid

#241290

Purpose

Allows co-translational incorporation of the fluorescent ncAA Anap directly into the target protein; expresses tRNACUA-Leu, AnapRS, and dominant negative ribosomal release factor

Depositor

Justin Taraska

Article

Obashi et al Structure. Volume 34, Issue 1, 8 January

Insert

the amber suppressor tRNACUA-Leu

Expression

Mammalian

Mutation

mutant E.coli leucyl synthetase specific for Anap…

Promoter

CMV

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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anti-FLAG-CAR (human, optimised, tonic-signal-free)

Plasmid

#247778

Purpose

Anti-FLAG scFv with Myc-tag, human CD8α-truncated hinge, and human CD28-CD3z intracellular domain.

Depositor

Clare Slaney

Article

Zhang et al Clin Transl Immunology. 2025 Aug 21;14(8

Insert

antiFLAG-CAR4

Use

Lentiviral

Tags

Myc

Expression

Mammalian

Promoter

EF-1α

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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cBEST2

Plasmid

#234658

Purpose

Expresses cytosine base editor - spCas9n (D10A) fused to APOBEC1 and UGI, Include Golden Gate compatible cassette for sgRNA insertion

Depositor

Mingzi M. Zhang

Article

Lin et al Appl Environ Microbiol. 2025 Mar 10:e019

Inserts

spCas9 cytosine base editor
Golden Gate compatible sgRNA insertion cassette

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

kasO*17tss and kasOp*

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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TRBV7-4

Plasmid

#237923

Purpose

Encodes TRBV7-4 allele and TRAC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al bioRxiv 2025.04.24.649726

Insert

TRBV7-4-TRAC (TRBV7-4 Human)

Use

Synthetic Biology

Mutation

None

Available Since

Oct. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pYO1261

Plasmid

#235726

Purpose

Protein expression of His6-SUMO-human RAB5A Q79L 1-211 in bacteria

Depositor

Roger Williams

Article

Tremel et al Nat Commun. 2021 Mar 10;12(1):1564. doi:

Insert

RAB5A (RAB5A Human)

Tags

6xHis-SUMO-TEV

Expression

Bacterial

Mutation

Q79L, aa 1-211

Available Since

Oct. 24, 2025

Availability

Academic Institutions and Nonprofits only

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TRE3G-ZIM3-Cas9-P2A-GFP

Plasmid

#239605

Purpose

Doxycycline inducible CRISPRgenee construct

Depositor

Philipp Rathert

Article

Stadager et al Cell Rep Methods. 2025 Jun 16;5(6):10107

Insert

ZIM3 KRAB domain (ZIM3 )

Use

CRISPR and Lentiviral

Promoter

TRE3G

Available Since

Sept. 30, 2025

Availability

Academic Institutions and Nonprofits only

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