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CROP_C9_Puro_JAK2

Plasmid

#183303

Purpose

All-in-One CRISPRko system with a guide RNA that targets JAK2 gene

Depositor

Prashant Mali

Publication

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

JAK2

Use

Lentiviral

Available since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_IDH2

Plasmid

#183301

Purpose

All-in-One CRISPRko system with a guide RNA that targets IDH2 gene

Depositor

Prashant Mali

Publication

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

IDH2

Use

Lentiviral

Available since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_HDAC3

Plasmid

#183299

Purpose

All-in-One CRISPRko system with a guide RNA that targets HDAC3 gene

Depositor

Prashant Mali

Publication

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

HDAC3

Use

Lentiviral

Available since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_FLT3

Plasmid

#183293

Purpose

All-in-One CRISPRko system with a guide RNA that targets FLT3 gene

Depositor

Prashant Mali

Publication

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

FLT3

Use

Lentiviral

Available since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_CSF1R

Plasmid

#183277

Purpose

All-in-One CRISPRko system with a guide RNA that targets CSF1R gene

Depositor

Prashant Mali

Publication

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

CSF1R

Use

Lentiviral

Available since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pBTK150

Plasmid

#183126

Purpose

BTK Type 2 promoter part for golden gate assembly

Depositor

Jeffrey Barrick

Publication

Leonard et al Science. 2020 Jan 31;367(6477):573-576.

Insert

Reverse rnpB-T1 terminator and forward CP25 promoter for dsRNA expression

Use

Synthetic Biology

Available since

May 10, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pCVD013

Plasmid

#159757

Purpose

Expression vector for wild-type Fc-fused ACE2(614)

Depositor

Jim Wells

Publication

Glasgow et al Proc Natl Acad Sci U S A. 2020 Nov 10;11

Insert

ACE2 (ACE2 Human)

Expression

Mammalian

Available since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCS4127

Plasmid

#131746

Purpose

Folinic acid switch Fol-UGAAG

Depositor

Christina Smolke

Publication

Xiang et al Nat Commun. 2019 Sep 23;10(1):4327. doi:

Insert

Folinic acid switch Fol-UGAAG

Expression

Mammalian

Available since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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pMT Lifeact-TagBFP

Plasmid

#126664

Purpose

Inducible expression of Lifeact-TagBFP in S2 cells

Depositor

Ron Vale

Publication

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Lifeact-TagBFP fusion

Expression

Insect

Available since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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p33Fn

Plasmid

#158155

Purpose

Expression of crRNA array and FnCas12a using one Pol II promoter. For plant genome editing.

Depositor

Kabin Xie

Publication

Ding et al Mol Plant. 2018 Apr 2;11(4):542-552. doi

Type

Empty backbone

Use

CRISPR

Expression

Plant

Mutation

Rice codon optimized

Available since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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OA-986B

Plasmid

#124999

Purpose

Expresses dCas9-VPR driven by the Ubiquitin-63E promoter

Depositor

Omar Akbari

Publication

Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

Insert

dCas9-VPR

Use

Piggyback

Expression

Insect

Promoter

Ubiquitin

Available since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pCre-AntiDsRed

Plasmid

#139674

Purpose

Cassette for testing Cre recombinase activity using a DsRed2 fluorescent reporter

Depositor

James Birchler

Publication

Cody et al Plant Direct. 2020 Mar 9;4(3):e00209. do

Insert

AntiDsRed Cre recombinase

Expression

Plant

Promoter

Maize Ubiquitin

Available since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ239A (dFnCas12a)-TV

Plasmid

#136379

Purpose

CRISPRa Gateway entry clone for catalytically dead FnCas12a fused with TV activation system

Depositor

Yiping Qi

Publication

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Insert

dFnCas12a-TV

Use

CRISPR

Expression

Plant

Mutation

D917A

Available since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pBTK620

Plasmid

#110620

Purpose

BTK assembled broad-host-range plasmid encoding GFP driven by CP32 promoter

Depositor

Jeffrey Barrick

Publication

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

GFP

Expression

Bacterial

Available since

Jan. 22, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pNP1 OR sfGFP

Plasmid

#107364

Purpose

Expresses sfGFP (a GFP) in Ecoli off a T7 promoter when pNP1 OR input A and pNP1 OR input B are co-expressed

Depositor

James Collins

Publication

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

OR gate + sfGFP

Expression

Bacterial

Promoter

T7

Available since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pNP1 OR input A

Plasmid

#107365

Purpose

Input A for pNP1 OR gate sfGFP

Depositor

James Collins

Publication

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

OR gate input A

Expression

Bacterial

Promoter

T7

Available since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pNP1 OR input B

Plasmid

#107366

Purpose

Input B for pNP1 OR gate sfGFP

Depositor

James Collins

Publication

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

OR gate input B

Expression

Bacterial

Promoter

T7

Available since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pBTK621

Plasmid

#110621

Purpose

BTK assembled broad-host-range plasmid encoding GFP driven by CP6 promoter

Depositor

Jeffrey Barrick

Publication

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

GFP

Expression

Bacterial

Available since

Sept. 21, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pBTK550d

Plasmid

#110617

Purpose

BTK assembled broad-host-range plasmid encoding inducible GFP driven by T7 polymerase

Depositor

Jeffrey Barrick

Publication

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

GFP

Expression

Bacterial

Available since

Aug. 10, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pDM1049

Plasmid

#108992

Purpose

Inducible expression vector (dox-on), C-terminal GFP tagging

Depositor

Rob Kay

Publication

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Dictyostelium discoideum inducible expression vec…

Tags

GFP

Available since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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