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We narrowed to 42,329 results for: CMV

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Showing: 6141 - 6160 of 42329 results

  6. pJEP321-pAAV-FullU6TO-SaCas9gRNAi(SapI)-CMV-TetR-P2A-GFP-KASH-WPRE-shortPA Empty Cassette

    Plasmid
    #113698
    Purpose
    Dox-inducible U6 SaCas9 gRNA expression cassette without a gRNA. Followed by an EFS driven GFP-KASH in a separate reading frame. SapI can be used to clone in gRNAs.
    Depositor
    Jonathan Ploski
    Article
    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.
    Inserts
    SaCas9 gRNA Cassete
    GFP
    Use
    AAV and CRISPR
    Tags
    KASH
    Available Since
    Feb. 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    113698-plasmid-map-sequence-id-232033

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  7. pMT647 human L1 ORF2p-3xFlag only (ORFeus-Hs, CMV promoter, monocistronic construct) in pCEP4 Puro (derivative of pMT646)

    Plasmid
    #213029
    Purpose
    Expresses human LINE-1 ORF2 only in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2
    Depositor
    Martin S. Taylor
    Article
    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:
    Insert
    Human LINE-1 ORF2p only (monocistronic, codon optimized ORFeus-Hs sequence) with ORF2-3xFlag
    Tags
    3xFlag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    213029-plasmid-map-sequence-id-422050

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  8. pMT1093 human L1 EN- (ORF2p E43S, D145N) ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro (derivative of pMT646)

    Plasmid
    #213028
    Purpose
    Expresses endonuclease dead (EN- ORF2p E43S, D145N) full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2
    Depositor
    Martin S. Taylor
    Article
    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:
    Insert
    Human LINE-1 (ORFeus-Hs codon optimized sequence, EN- E43S D145N) with ORF2-3xFlag
    Tags
    3xFlag (ORF2p)
    Expression
    Mammalian
    Mutation
    E43S D145N double mutant EN- endonuclease catalyt…
    Promoter
    CMV
    Available Since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    213028-plasmid-map-sequence-id-422046

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  10. pCMV-PDGFR-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq-TetON)(TRE-SEAP

    Plasmid
    #210513
    Purpose
    expresses PDGFR-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq-TetON and SEAP reporter in mammalian cells
    Depositor
    Dongmin Lee
    Article
    Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d
    Inserts
    secreted alkaline phosphatase
    CMV-PDGFR-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq
    Tags
    Myc
    Expression
    Mammalian
    Promoter
    CMV and TetO
    Available Since
    Jan. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    210513-plasmid-map-sequence-id-415575

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  11. Click editor utilizing mSA for template recruitment and ePhi29 DNA polymerase - pCMV-T7-mSA-nCas9-ePhi29 (JO1398)

    Plasmid
    #217803
    Purpose
    Variant CE1 construct with mSA for template recruitment and ePhi29(D169A) DNA pol, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    mSA-linker-SV40NLS-nSpCas9-BPNLS-ePhi29-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); Phi29(D169A/M8R/V51A/M97T/G197D/E…
    Promoter
    CMV and T7
    Available Since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217803-plasmid-map-sequence-id-432890

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  13. pCMV-NTOM20-EGFP-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq--bArr2-TevCs

    Plasmid
    #210516
    Purpose
    expresses NTOM20-EGFP-pCMV-PDGFR-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq--bArr2-TevCs component in mammalian cells
    Depositor
    Dongmin Lee
    Article
    Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d
    Insert
    NTOM20-EGFP-pCMV-PDGFR-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq--bArr2-TevCs
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    210516-plasmid-map-sequence-id-415570

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  14. pUC57-ITR2-H1- sgCTG Merry-eCMV-SaSpD10A+linker (Neuron codon optimization)-3XFLAG-SV40 NLS-ITR2

    Plasmid
    #210736
    Purpose
    Coding for SaSp D10A Cas9 alongside Merry sgRNA targeting CAG repeats
    Depositor
    Vincent Dion
    Article
    Mangin et al bioRxiv 2023.10.24.563270
    Inserts
    SaSp D10A Cas9
    Merry sgCAG
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, PolyA signal
    Expression
    Mammalian
    Mutation
    D10A, N-terminal Sa Cas9, C-terminal Sp Cas9
    Promoter
    H1 and eCMV
    Available Since
    Jan. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    210736-plasmid-map-sequence-id-420081

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  15. R620-M05-303: CMV51p> TPAss-SARS-CoV-2 S-RBD(319-541)-His6 K417N/E484K/N501Y

    Plasmid
    #170191
    Purpose
    Mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD), triple mutant from B.1.351 variant
    Depositor
    Dominic Esposito
    Article
    Mehalko et al Protein Expr Purif. 2021 Mar;179:105802.
    Insert
    SARS-CoV-2 RBD (S SARS-CoV-2)
    Tags
    His6
    Expression
    Mammalian
    Mutation
    K417N, E484K, N501Y
    Promoter
    CMV51
    Available Since
    May 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    170191-plasmid-map-sequence-id-334254

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  17. MF64_PB-TRE3G-6x42bs-6x(37bs_37bs)-miniCMV-NLS-FKBP12F36V-37ZFR2AR11AR39AR67A-VP16-NLS-DHFR-IRES-mCitrine-PEST-BGHpA

    Plasmid
    #176624
    Purpose
    TF B self-activation cassette in MultiFate-2.1 and MultiFate-2.3
    Depositor
    Michael Elowitz
    Article
    Zhu et al Science. 2022 Jan 21;375(6578):eabg9765.
    Insert
    TRE3G-6x42bs-6x(37bs_37bs)-miniCMV-NLS-FKBP12F36V-37ZFR2AR11AR39AR67A-VP16-NLS-DHFR-IRES-mCitrine-PEST-BGHpA
    Use
    Synthetic Biology
    Available Since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    176624-plasmid-map-sequence-id-347134

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  19. R620-M06-303: CMV51p> TPAss-SARS-CoV-2 S-RBD(319-529)-3C-His8-SBP K417N/E484K/N501Y

    Plasmid
    #170192
    Purpose
    Mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD), triple mutant from B.1.351 variant
    Depositor
    Dominic Esposito
    Article
    Mehalko et al Protein Expr Purif. 2021 Mar;179:105802.
    Insert
    SARS-CoV-2 RBD (S SARS-CoV-2)
    Tags
    3C-His8-SBP
    Expression
    Mammalian
    Mutation
    K417N, E484K, N501Y
    Promoter
    CMV51
    Available Since
    May 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    170192-plasmid-map-sequence-id-334252

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  20. pCMV-NTOM20-SbMVpC-43LK-iLID(139)-NES-NES-SbMVpN-iLID-SbMVseq-bArr2-TevCs-P2A-EGFP

    Plasmid
    #210505
    Purpose
    expresses NTOM20-SbMVpC-43LK-iLID(139)-NES-NES-SbMVpN-iLID-SbMVseq-bArr2-TevCs-P2A-EGFP component in mammalian cells
    Depositor
    Dongmin Lee
    Article
    Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d
    Insert
    NTOM20-SbMVpC-43LK-iLID(139)-NES-NES-SbMVpN-iLID-SbMVseq-bArr2-TevCs-P2A-EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    210505-plasmid-map-sequence-id-422559

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Showing: 6141 - 6160 of 42329 results