We narrowed to 11,723 results for: Vars

1. pDONR223_NFKBIB_WT

   Plasmid

   #82219

   Purpose

   Gateway Donor vector containing NFKBIB , part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, William Hahn, David Root

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   NFKBIB (NFKBIB Human)
   

   Use

   Gateway entry vector

   Mutation

   WT

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-Flag-RNF168 delta MIU1/MIU2

   Plasmid

   #133981

   Purpose

   mammalian expression vector of Flag tagged RNF168 where both motifs interacting with ubiquitin have been deleted

   Depositor

   Daniel Durocher

   Article

   Stewart et al Cell. 2009 Feb 6;136(3):420-34. doi: 10.

   Insert

   RNF168 (RNF168 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   deletion of MIU1 and MIU2

   Promoter

   CMV

   Available Since

   Nov. 4, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pDONR223_WNT2_WT

   Plasmid

   #81775

   Purpose

   Gateway Donor vector containing WNT2 , part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, William Hahn, David Root

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   WNT2 (WNT2 Human)
   

   Use

   Gateway entry vector

   Mutation

   WT

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pZE21/UBP1/ClpS

   Plasmid

   #98566

   Purpose

   Expresses truncated codon-opt S. cerevisiae UBP1 and E. coli ClpS in an artificial operon

   Depositor

   George Church

   Article

   Kunjapur et al Proc Natl Acad Sci U S A. 2018 Jan 16;11

   Inserts

   Truncated ubiquitin cleavase, codon optimized
   ATP-dependent Clp protease adapter protein
   

   Expression

   Bacterial

   Mutation

   In a synthetic operon downstream of UBP1 and Remo…

   Promoter

   Same as UBP1 (operon) and Tet promoter (aTc induc…

   Available Since

   Feb. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. AT1G35710 X075_pECIA2

   Plasmid

   #115098

   Purpose

   Bait vector AT1G35710 X075_pECIA2 should be used with prey vector AT1G35710 X075_pECIA14.

   Depositor

   Youssef Belkhadir

   Article

   Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

   Insert

   AT1G35710 (AT1G35710 Mustard Weed)
   

   Tags

   Fc, V5 and hexahistidine tags

   Expression

   Insect

   Promoter

   Metallothionein (Copper-Inducible)

   Available Since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. AT1G56140 X032_pECIA2

   Plasmid

   #115062

   Purpose

   Bait vector AT1G56140 X032_pECIA2 should be used with prey vector AT1G56140 X032_pECIA14.

   Depositor

   Youssef Belkhadir

   Article

   Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

   Insert

   AT1G56140 (AT1G56140 Mustard Weed)
   

   Tags

   Fc, V5 and hexahistidine tags

   Expression

   Insect

   Promoter

   Metallothionein (Copper-Inducible)

   Available Since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. AT1G06840 X062_pECIA2

   Plasmid

   #115052

   Purpose

   Bait vector AT1G06840 X062_pECIA2 should be used with prey vector AT1G06840 X062_pECIA14.

   Depositor

   Youssef Belkhadir

   Article

   Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

   Insert

   AT1G06840 (AT1G06840 Mustard Weed)
   

   Tags

   Fc, V5 and hexahistidine tags

   Expression

   Insect

   Promoter

   Metallothionein (Copper-Inducible)

   Available Since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. AT2G19230 M11_pECIA2

   Plasmid

   #114949

   Purpose

   Bait vector AT2G19230 M11_pECIA2 should be used with prey vector AT2G19230 M11_pECIA14.

   Depositor

   Youssef Belkhadir

   Article

   Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

   Insert

   AT2G19230 (AT2G19230 Mustard Weed)
   

   Tags

   Fc, V5 and hexahistidine tags

   Expression

   Insect

   Promoter

   Metallothionein (Copper-Inducible)

   Available Since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. AT1G29730 X066_pECIA14

   Plasmid

   #114855

   Purpose

   Prey vector AT1G29730 X066_pECIA14 should be used with bait vector AT1G29730 X066_pECIA2.

   Depositor

   Youssef Belkhadir

   Article

   Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

   Insert

   AT1G29730 (AT1G29730 Mustard Weed)
   

   Expression

   Insect

   Promoter

   Metallothionein (Copper-Inducible)

   Available Since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. AT3G02880 J3_pECIA14

    Plasmid

    #114790

    Purpose

    Prey vector AT3G02880 J3_pECIA14 should be used with bait vector AT3G02880 J3_pECIA2.

    Depositor

    Youssef Belkhadir

    Article

    Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

    Insert

    AT3G02880 (AT3G02880 Mustard Weed)
    

    Expression

    Insect

    Promoter

    Metallothionein (Copper-Inducible)

    Available Since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. AT1G51890 M5_pECIA14

    Plasmid

    #114744

    Purpose

    Prey vector AT1G51890 M5_pECIA14 should be used with bait vector AT1G51890 M5_pECIA2.

    Depositor

    Youssef Belkhadir

    Article

    Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

    Insert

    AT1G51890 (AT1G51890 Mustard Weed)
    

    Expression

    Insect

    Promoter

    Metallothionein (Copper-Inducible)

    Available Since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPB-cT3G-cERP2-FOSB

    Plasmid

    #192901

    Purpose

    Barcoded piggybac transposon vector with Dox-inducible expression of FOSB

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    FOSB (FOSB Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    TET3G-miniCMV

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV.CAG-FLEX.SF-Venus-iGluSnFR.A184V

    Plasmid

    #106190

    Purpose

    Medium affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Insert

    SF-Venus-iGluSnFR.A184V
    

    Use

    AAV

    Mutation

    SFGFP: T203Y, F46L, T65G, S72A GltI: A184V

    Promoter

    CAG

    Available Since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDONR221 SARS-CoV-2 S-frag5_nostop

    Plasmid

    #153177

    Purpose

    Gateway-compatible Entry vector, with a fragmented insert of S gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

    Depositor

    Fritz Roth, Mikko Taipale

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    S-frag5
    

    Use

    Gateway-compatible entry vector

    Mutation

    Many synonymous changes due to codon optimization

    Available Since

    May 27, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. LV_EF1a_Stat3(A662C,N664C,V667L)-P2A-Hygro_Barcode

    Plasmid

    #170254

    Purpose

    Barcoded lentiviral vector to express Stat3 (A662C, N664C, V667L) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

    Depositor

    Prashant Mali

    Article

    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

    Insert

    STAT3 (STAT3 Human)
    

    Use

    Lentiviral

    Mutation

    A662C, N664C, V667L

    Promoter

    EF1a

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pS6-TGFB2-STREP

    Plasmid

    #128503

    Purpose

    Expresses human pro-TGFβ2 (from L21 to S414) in mammalian cells. With N-t STREP(2x) tag.

    Depositor

    F Xavier Gomis-Ruth

    Article

    Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

    Insert

    TGFB2 (TGFB2 Human)
    

    Tags

    STREP (2X) tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDONR223 SARS-CoV2 TEV-N

    Plasmid

    #154424

    Purpose

    Gateway-compatible Entry vector

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    N (N SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Gateway-compatible entry vector, with insert of n…

    Mutation

    Many synonymous changes due to codon optimization

    Available Since

    Sept. 2, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pDONR223_SV2C_WT

    Plasmid

    #81924

    Purpose

    Gateway Donor vector containing SV2C, part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, William Hahn, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    SV2C (SV2C Human)
    

    Use

    Gateway entry vector

    Mutation

    F346S

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPGK-T7/2-CD44v3 (-cyt)

    Plasmid

    #137813

    Purpose

    expression of CD44 proteins in mammalian cells

    Depositor

    Ursula Gunthert

    Article

    Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

    Insert

    CD44v3 (-cyt) (CD44 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    without cytoplasmic region, N280D in the V3 region

    Promoter

    PGK

    Available Since

    June 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCC_10 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-KRAB-dCas9NG-NLS-2A-Puro-WPRE

    Plasmid

    #139095

    Purpose

    Expresses human codon-optimized inactive SpCas9-NG fused to a transcriptional repressor KRAB in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.

    Depositor

    Neville Sanjana

    Article

    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

    Insert

    KRAB-dSpCas9-NG
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    D10A, H840A, L1111R, D1135V,G1218R, E1219F, A1322…

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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