We narrowed to 23,485 results for: LEA

1. C-Terminal Split Cas9 with GyrA intein

   Plasmid

   #58694

   Purpose

   Expresses truncated C-terminal SpCas9 domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with N-Terminal Split Cas9 Gyra Intein for full length SpCas9 production

   Depositor

   Gang Bao

   Article

   Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103

   Insert

   Humanized C-Terminal S. pyogenes Cas9 with GyrA Csplit Intein
   

   Use

   AAV and CRISPR

   Tags

   GyrA Csplit Intein and NLS

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   July 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTRIPZ shSTING-Hsa

   Plasmid

   #128158

   Purpose

   Doxycyclin inducible shRNA knockdown of human STING gene

   Depositor

   Sandra Demaria

   Article

   Vanpouille-Box et al Nat Commun. 2017 Jun 9;8:15618. doi: 10.

   Insert

   shSTING Hsa (STING1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFUSEss-CHIg-mM_M18

   Plasmid

   #91729

   Purpose

   Expression plasmid coding for heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18.

   Depositor

   Joanna Bereta

   Article

   Klaus et al Sci Rep. 2018 Jan 11;8(1):519. doi: 10.1

   Insert

   immunoglobulin heavy constant mu (Ighm Mouse)
   

   Expression

   Mammalian

   Promoter

   hEF1-HTLV prom

   Available Since

   June 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pINDUCER20-α-Syn

   Plasmid

   #92200

   Purpose

   Retroviral overexpression vector (doxycycline-inducible) for α-Syn

   Depositor

   Huda Zoghbi

   Article

   Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

   Insert

   SNCA (SNCA Human)
   

   Use

   Lentiviral

   Tags

   Myc

   Expression

   Mammalian

   Available Since

   June 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-hSyn Con/Fon hChR2(H134R)-EYFP

   Plasmid

   #55645

   Purpose

   Cre-on/Flp-on ChR2-EYFP under the Synapsin promoter

   Depositor

   Karl Deisseroth

   Article

   Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

   Has Service

   AAV Retrograde, AAV5, AAV8, and AAV9

   Insert

   ChR2(H134R)-EYFP
   

   Use

   AAV; Cre on/flp on chr2-eyfp

   Tags

   EYFP

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   Aug. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. YFP NLS Beta-Actin G13R

   Plasmid

   #60615

   Purpose

   Encodes YFP and NLS tagged beta-actin with the G13R depolymerization mutation

   Depositor

   Primal de Lanerolle

   Article

   Chang et al Proc Natl Acad Sci U S A. 2011 May 24;10

   Insert

   Beta-actin (ACTB Human)
   

   Tags

   EYFP and NLS

   Expression

   Mammalian

   Mutation

   G13R

   Available Since

   Nov. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA5-eGFP-PC1-3HA

   Plasmid

   #108406

   Purpose

   Can be used to generate stable Flp-In cells with tetracycline-inducible human PC1 expression.

   Depositor

   Gregory Germino

   Article

   Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

   Insert

   PKD1 (PKD1 Human)
   

   Tags

   3HA and eGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pC13N-iCAG.copGFP

   Plasmid

   #66578

   Purpose

   CLYBL safe harbor gene targeting donor expressing CAG-driven copGFP, compatible with pZT-C13 TALENs

   Depositor

   Jizhong Zou

   Article

   Cerbini et al PLoS One. 2015 Jan 14;10(1):e0116032. do

   Insert

   copGFP
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   June 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHR-BRD4ΔN-mCherry-sspB

   Plasmid

   #121968

   Purpose

   Expresses fusion of disordered protein BRD4(462-1362), fluorescent protein mCherry, and sspB which upon light activation binds to iLID.

   Depositor

   Clifford Brangwynne

   Article

   Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

   Insert

   BRD4 (BRD4 Human)
   

   Use

   Lentiviral

   Tags

   mCherry-sspB

   Expression

   Mammalian

   Mutation

   Deleted amino acids 1-461

   Promoter

   SFFV

   Available Since

   April 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. YFP NLS Beta-Actin S14C

    Plasmid

    #60614

    Purpose

    Encodes YFP and NLS tagged beta-actin with the S14C polymerization mutation

    Depositor

    Primal de Lanerolle

    Article

    Chang et al Proc Natl Acad Sci U S A. 2011 May 24;10

    Insert

    Beta-actin (ACTB Human)
    

    Tags

    EYFP and NLS

    Expression

    Mammalian

    Mutation

    S14C

    Available Since

    Nov. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAVS1P-iCLHN

    Plasmid

    #66579

    Purpose

    AAVS1 safe harbor gene targeting donor expressing CAG-driven Nanoluc-HaloTag, compatible with pZT-AAVS1 TALENs

    Depositor

    Jizhong Zou

    Article

    Cerbini et al PLoS One. 2015 Jan 14;10(1):e0116032. do

    Insert

    Nanoluc-HaloTag
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. 6-His-MBP-TEV-FnCpf1

    Plasmid

    #90094

    Purpose

    Bacterial expression plasmid for protein purification

    Depositor

    Feng Zhang

    Article

    Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

    Insert

    FnCpf1 (humanized)
    

    Tags

    3xHA, 6xHis, MBP, Nucleoplasmin NLS, and TEV site…

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAVS1P-iCAG.copGFP

    Plasmid

    #66577

    Purpose

    AAVS1 safe harbor gene targeting donor expressing CAG-driven copGFP, compatible with pZT-AAVS1 TALENs

    Depositor

    Jizhong Zou

    Article

    Cerbini et al PLoS One. 2015 Jan 14;10(1):e0116032. do

    Insert

    copGFP
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDNA3 IRF7(5’UTR)-FF

    Plasmid

    #85490

    Purpose

    Firefly luciferase under the control of IRF7 5'UTR

    Depositor

    Ivan Topisirovic

    Article

    Gandin et al Genome Res. 2016 May;26(5):636-48. doi:

    Insert

    5'UTR of IRF7 (Irf7 Mouse)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Available Since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA-flag-hKDM4A-polyA

    Plasmid

    #101051

    Purpose

    in vitro transcription (T7 promoter)

    Depositor

    Yi Zhang

    Article

    Chung et al Cell Stem Cell. 2015 Dec 3;17(6):758-66.

    Insert

    KDM4A (KDM4A Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    T7

    Available Since

    Nov. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGL403

    Plasmid

    #119953

    Purpose

    Expresses GPPS and LimS, for the production of GPP and (S)-limonene, in Escherichia coli.

    Depositor

    Nigel Scrutton

    Article

    Jervis et al ACS Synth Biol. 2018 Dec 18. doi: 10.102

    Inserts

    gpps
    limS
    

    Expression

    Bacterial

    Mutation

    N-terminal truncation of 56 amino acid residues (…

    Available Since

    March 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcrDNA3.1-PRKACA

    Plasmid

    #100890

    Purpose

    Mammalian expression of PRKACA

    Depositor

    Sanford Simon

    Article

    Honeyman et al Science. 2014 Feb 28;343(6174):1010-4. d

    Insert

    Protein Kinase CAMP-Activated Catalytic Subunit Alpha (PRKACA Human)
    

    Expression

    Mammalian

    Mutation

    Isoform 1 wild type

    Promoter

    CMV

    Available Since

    Sept. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-hSyn Coff/Fon hChR2(H134R)-EYFP

    Plasmid

    #55648

    Purpose

    Cre-off/Flp-on ChR2-EYFP under the Synapsin promoter

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Insert

    ChR2(H134R)-EYFP
    

    Use

    AAV; Cre off/flp on chr2-eyfp

    Tags

    EYFP

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    Aug. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLL3.7-EF-EYFP-YAP1_WT-PolyA

    Plasmid

    #112284

    Purpose

    Fluorescently labels WT human YAP1-2gamma isoform (504 aa)

    Depositor

    Erik Sahai

    Article

    Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.

    Insert

    EYFP-YAP1 (YAP1 Human)
    

    Use

    Lentiviral

    Mutation

    WT human YAP1-2gamma

    Promoter

    EF-1 alpha

    Available Since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-hARdelta538-614

    Plasmid

    #89107

    Purpose

    mammalian expression of human androgen receptor with deletion of amino acids 538-614, deletion of AR DNA binding domain

    Depositor

    Elizabeth Wilson

    Article

    Zhou et al J Biol Chem. 1994 May 6;269(18):13115-23

    Insert

    hAR-538-614 (AR Human)
    

    Expression

    Mammalian

    Mutation

    hAR delta 538-614

    Promoter

    CMV

    Available Since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only