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pcDNA3.3-hALDOB

Plasmid

#246127

Purpose

For transient transfection of cells for expression of human ALDOB

Depositor

Sheng-cai Lin

Article

Zhang et al Nature. 2017 Aug 3;548(7665):112-116. do

Insert

ALDOB (ALDOB Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

April 21, 2026

Availability

Academic Institutions and Nonprofits only

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pN1_HsKIF1C(1-376)-NC-LZ-mNG

Plasmid

#253325

Purpose

mammalian expression of the motor domain (aa 1-376) of human KIF1C tagged with mNG. The NC and LZ sequences facilitate stable dimerization of the motor domains.

Depositor

Kristen Verhey

Article

Geng et al J Cell Biol. 2025 Oct 6;224(10):e2025010

Insert

HsKIF1C(1-376) (KIF1C Human)

Tags

mNeonGreen

Expression

Mammalian

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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pN1_HsKIF1C(349-1103)-mNG

Plasmid

#253327

Purpose

mammalian expression of the stalk+tail domains of human KIF1C (aa 349-1103) tagged with mNG.

Depositor

Kristen Verhey

Article

Geng et al J Cell Biol. 2025 Oct 6;224(10):e2025010

Insert

HsKIF1C(349-1103) (KIF1C Human)

Tags

mNeonGreen

Expression

Mammalian

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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pN1_HsKIF1C(886-1103)-mNG

Plasmid

#253328

Purpose

mammalian expression of the IDR domain of human KIF1C (aa 886-1103) tagged with mNG.

Depositor

Kristen Verhey

Article

Geng et al J Cell Biol. 2025 Oct 6;224(10):e2025010

Insert

HsKIF1C(886-1103) (KIF1C Human)

Tags

mNeonGreen

Expression

Mammalian

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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pIVT_hifiDn29-P2A-GFP

Plasmid

#247164

Purpose

In vitro transcription of human codon optimized engineered hifiDn29-P2A-GFP

Depositor

Patrick Hsu

Article

Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038

Insert

hifiDn29-P2A-GFP

Use

In vitro transcription

Tags

6xHis

Mutation

M6I/E70G/A224P/G227V/Q332K/N341K/L393P/D503N/I98V…

Promoter

mutated T7

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pEf1a-hifiDn29-P2A-GFP

Plasmid

#247154

Purpose

Mammalian expression of a human codon optimized engineered hifiDn29 recombinase (highest specificity variant)

Depositor

Patrick Hsu

Article

Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038

Insert

hifiDn29-P2A-GFP

Tags

6xHis

Expression

Mammalian

Mutation

M6I/E70G/A224P/G227V/Q332K/N341K/L393P/D503N/I98V…

Promoter

Ef1a

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.3 C-HA ZO1

Plasmid

#227883

Purpose

Used for transfection of cells for expression of N-HA ZO1

Depositor

Sheng-cai Lin

Article

Li et al Cell Res. 2024 Jun 19. doi: 10.1038/s414

Insert

ZO1 (TJP1 Human)

Tags

HA

Expression

Mammalian

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pSmBtA3-d7F

Plasmid

#245784

Purpose

Expression of mutated bovine arrestin-3 (deleted residues 175, 177, 178, 182-185) tagged with SmBiT and Flag in mammalian cells

Depositor

Vsevolod Gurevich

Article

Zheng et al Cells. 2023 Jun 6;12(12):1563. doi: 10.3

Insert

arrestin-3 (ARR3 Bovine)

Tags

Flag and SmBiT

Expression

Mammalian

Mutation

deleted residues 175, 177, 178, 182-185

Promoter

CMV

Available Since

Oct. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pFB-Dual-MBP-NavAb (KAV/G94C/Q150C)

Plasmid

#226884

Purpose

Expresses a mutant of the Arcobacter butzleri voltage-gated sodium channel fused to an MBP tag in insect cells

Depositor

Ning Zheng

Article

Wisedchaisri et al Cell. 2019 Aug 8;178(4):993-1003.e12. do

Insert

NaVAb

Tags

MBP

Expression

Insect

Mutation

R4A, N49K, L109A, M116V

Promoter

Polyhedrin

Available Since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pFB-D3-ASK1

Plasmid

#226619

Purpose

Co-expression of D3 and ASK1 complex in insect cells

Depositor

Ning Zheng

Article

Shabek et al Nature. 2018 Nov;563(7733):652-656. doi:

Inserts

D3
ASK1 (SKP1 Mustard Weed)

Tags

His tag followed with three copies of Msyb

Expression

Insect

Mutation

The flexible loop in D3 (Q478-D515) can be remove…

Promoter

Polyhedrin

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pAdEasy-hALDOA K230A

Plasmid

#210814

Purpose

To block the formation of the initial Schiff base with FBP

Depositor

Sheng-cai Lin

Article

Zhang et al Nature. 2017 Aug 3;548(7665):112-116. do

Insert

ALDOA (ALDOA Human)

Use

Adenoviral

Tags

HA

Mutation

residues 230 of ALDOA mutated to alanine

Promoter

CMV

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-Bcl-2-K17R-K22R

Plasmid

#220294

Purpose

mammalian expression of Bcl-2-K17R-K22R tagged with GFP

Depositor

Xinlai Cheng

Article

Wang et al Cell Reports Physical Science, Volume 5,

Insert

Bcl2 (BCL2 Human)

Tags

Emerald GFP

Expression

Mammalian

Mutation

K17R-K22R

Promoter

CMV

Available Since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-Bcl-2-G128L-A131L-T132L

Plasmid

#220304

Purpose

mammalian expression of Bcl-2-G128L-A131L-T132L tagged with GFP

Depositor

Xinlai Cheng

Article

Wang et al Cell Reports Physical Science, Volume 5,

Insert

Bcl2 (BCL2 Human)

Tags

Emerald GFP

Expression

Mammalian

Mutation

G128L-A131L-T132L

Promoter

CMV

Available Since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-deltaExon17

Plasmid

#209563

Purpose

Mammalian expression of Shtn1 long isoform with E17 deletion

Depositor

Sika Zheng

Article

Zhang et al Neuron. 2019 Feb 20;101(4):690-706.e10.

Insert

Shtn1 long isoform with E17 deletion (4930506M07Rik Mouse)

Expression

Mammalian

Mutation

Exon17 deletion

Available Since

March 7, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-SHTN1LRRR>GGG

Plasmid

#209562

Purpose

Mammalian expression of Shtn1 long isoform with RG mutation

Depositor

Sika Zheng

Article

Zhang et al Neuron. 2019 Feb 20;101(4):690-706.e10.

Insert

Shtn1 long isoform with RG mutation (4930506M07Rik Mouse)

Expression

Mammalian

Mutation

RRR to GGG

Available Since

March 7, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-deltaExon16-17

Plasmid

#209564

Purpose

Mammalian expression of Shtn1 long isoform with E16-17 deletion

Depositor

Sika Zheng

Article

Zhang et al Neuron. 2019 Feb 20;101(4):690-706.e10.

Insert

Shtn1 long isoform with E16-17 deletion (4930506M07Rik Mouse)

Expression

Mammalian

Mutation

Exon16 and 17 deletion

Available Since

March 7, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-deltaExon15-II

Plasmid

#209566

Purpose

Mammalian expression of Shtn1 long isoform with E15 deletion II

Depositor

Sika Zheng

Article

Zhang et al Neuron. 2019 Feb 20;101(4):690-706.e10.

Insert

Shtn1 long isoform with E15 deletion II (4930506M07Rik Mouse)

Expression

Mammalian

Mutation

Exon15 deletion II

Available Since

March 5, 2024

Availability

Academic Institutions and Nonprofits only

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pBoBi-hALDOA K230A

Plasmid

#210808

Purpose

To block the formation of the initial Schiff base with FBP

Depositor

Sheng-cai Lin

Article

Zhang et al Nature. 2017 Aug 3;548(7665):112-116. do

Insert

ALDOA (ALDOA Human)

Use

Lentiviral

Tags

HA

Mutation

residues 230 of ALDOA mutated to alanine

Promoter

CMV

Available Since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pLINE1mORF1-ΔORF2-scFvFc

Plasmid

#206023

Purpose

The base next to the ORF1 start codon (ATG) was deleted in pLINE1ΔORF2-scFvFc

Depositor

Masamichi Kamihira

Article

Zheng et al Biotechnol J. 2021 Jul;16(7):e2000620. d

Insert

mouse LINE1 vector harboring an scFv-Fc expression unit, encoding mutated ORF1 but lacking ORF2

Expression

Mammalian

Available Since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pCAGIG-FLAG-SHTN1L

Plasmid

#209568

Purpose

Mammalian expression of Shtn1 long isoform

Depositor

Sika Zheng

Article

Zhang et al Neuron. 2019 Feb 20;101(4):690-706.e10.

Insert

Shtn1 long isoform (4930506M07Rik Mouse)

Expression

Mammalian

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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