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pVC-Ds-DrU6a:scrambled1-sgRNA-Ds

Plasmid

#119070

Purpose

Construct with scrambled-1 sgRNA sequence for microinjections in zebrafish

Depositor

Tatjana Sauka-Spengler

Publication

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Insert

U6a:scrambled1-sgRNA

Expression

Bacterial

Promoter

Zebrafish U6a promoter

Available since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pVC-Ds-DrU6a:scrambled2-sgRNA-Ds

Plasmid

#119071

Purpose

Construct with scrambled-2 sgRNA sequence for microinjections in zebrafish

Depositor

Tatjana Sauka-Spengler

Publication

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Insert

U6a:scrambled2-sgRNA

Expression

Bacterial

Promoter

Zebrafish U6a promoter

Available since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pVC-Ds-DrU6a:scrambled3-sgRNA-Ds

Plasmid

#119072

Purpose

Construct with scrambled-3 sgRNA sequence for microinjections in zebrafish

Depositor

Tatjana Sauka-Spengler

Publication

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Insert

U6a:scrambled3-sgRNA

Expression

Bacterial

Promoter

Zebrafish U6a promoter

Available since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pVC-Ds-DrU6a:scrambled4-sgRNA-Ds

Plasmid

#119073

Purpose

Construct with scrambled-4 sgRNA sequence for microinjections in zebrafish

Depositor

Tatjana Sauka-Spengler

Publication

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Insert

U6a:scrambled4-sgRNA

Expression

Bacterial

Promoter

Zebrafish U6a promoter

Available since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-GCN4cc-Erp2CT

Plasmid

#73923

Purpose

GST fusion- coiled coil- Erp2 cytoplasmic tail

Depositor

Blanche Schwappach

Publication

Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

Erp2 CT

Tags

GST

Expression

Bacterial

Promoter

tac

Available since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-GCN4cc-Erp1CT

Plasmid

#73921

Purpose

GST fusion- coiled coil- Erp1 cytoplasmic tail

Depositor

Blanche Schwappach

Publication

Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

Erp1 CT

Tags

GST

Expression

Bacterial

Promoter

tac

Available since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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p415 δCLD

Plasmid

#73876

Purpose

δCLD - His tagged (2α helices+μHD deleted)

Depositor

Blanche Schwappach

Publication

Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

δCLD

Tags

His

Expression

Yeast

Promoter

Met25

Available since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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P415 Ret2LD

Plasmid

#73875

Purpose

Ret2LD - His tagged (2α helices+μHD deleted)

Depositor

Blanche Schwappach

Publication

Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

Ret2LD

Tags

His

Expression

Yeast

Promoter

Met25

Available since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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TU#1805_CRISPR_unc-73_exon2

Plasmid

#82359

Purpose

to create unc-73B null allele

Depositor

Martin Chalfie

Publication

Zheng et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

Cas9 and sgRNA against unc-73 exon2 (unc-73 Nematode)

Use

CRISPR

Expression

Worm

Promoter

U6

Available since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pJC-TALE-VP64

Plasmid

#46147

Depositor

David Stern

Publication

Crocker et al Nat Methods. 2013 Aug;10(8):762-7. doi:

Type

Empty backbone

Tags

VP64 activation domain

Expression

Insect

Promoter

hsp70

Available since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDHJS3 AN+

Plasmid

#78240

Purpose

One of four plasmids needed to create the mismatched double Holliday junction substrate (MM-DHJS).

Depositor

Tao-shih Hsieh

Publication

Plank et al J Biol Chem. 2006 Jun 23;281(25):17510-6

Inserts

loxP
sequence "A" with NheI site
loxP2

Use

Phagemid

Available since

June 7, 2016

Availability

Academic Institutions and Nonprofits only

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plentiCRISPRv2 CTNNA1

Plasmid

#258469

Purpose

CRISPR-mediated knock-out of human CTNNA1

Depositor

Matthew Kutys

Publication

Mayo et al J Cell Biol. 2026 Jun 1;225(6):e20250314

Insert

CTNNA1 sgRNA

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available since

July 22, 2026

Availability

Academic Institutions and Nonprofits only

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plentiCRISPRv2 SCRIB

Plasmid

#258466

Purpose

CRISPR-mediated knock-out of human SCRIB

Depositor

Matthew Kutys

Publication

Mayo et al J Cell Biol. 2026 Jun 1;225(6):e20250314

Insert

SCRIB sgRNA

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available since

July 22, 2026

Availability

Academic Institutions and Nonprofits only

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plentiCRISPRv2 MYO1C

Plasmid

#258472

Purpose

CRISPR-mediated knock-out of human MYO1C

Depositor

Matthew Kutys

Publication

Mayo et al J Cell Biol. 2026 Jun 1;225(6):e20250314

Insert

MYO1C sgRNA

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available since

July 22, 2026

Availability

Academic Institutions and Nonprofits only

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plentiCRISPRv2 ERBIN

Plasmid

#258467

Purpose

CRISPR-mediated knock-out of human ERBIN

Depositor

Matthew Kutys

Publication

Mayo et al J Cell Biol. 2026 Jun 1;225(6):e20250314

Insert

ERBIN sgRNA

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available since

July 22, 2026

Availability

Academic Institutions and Nonprofits only

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plentiCRISPRv2 DLG5

Plasmid

#258468

Purpose

CRISPR-mediated knock-out of human DLG5

Depositor

Matthew Kutys

Publication

Mayo et al J Cell Biol. 2026 Jun 1;225(6):e20250314

Insert

DLG5 sgRNA

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available since

July 22, 2026

Availability

Academic Institutions and Nonprofits only

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pRRL VE-cadherin-α-catenin-mEmerald

Plasmid

#258470

Purpose

Lentiviral expression of mEmerald-tagged VE-cadherin-α catenin chimera

Depositor

Matthew Kutys

Publication

Mayo et al J Cell Biol. 2026 Jun 1;225(6):e20250314

Insert

VE-cadherin-α-catenin chimera

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available since

July 7, 2026

Availability

Academic Institutions and Nonprofits only

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pWUKI Cas1 Cas2 E9Q

Plasmid

#80091

Purpose

Encodes Cas1 and Cas2 E9Q

Depositor

George Church

Publication

Shipman et al Science. 2016 Jun 9. pii: aaf1175.

Inserts

Cas1 (cas1 )
Cas2 (cas2 )
CRISPR Array (bp1542-2899)

Expression

Bacterial

Mutation

E9Q

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pWUKI Cas1 V134L Cas2

Plasmid

#80092

Purpose

Encodes Cas1 V134L and Cas2

Depositor

George Church

Publication

Shipman et al Science. 2016 Jun 9. pii: aaf1175.

Inserts

Cas1 (cas1 )
Cas2 (cas2 )
CRISPR Array

Expression

Bacterial

Mutation

V134L

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pWUKI Cas1 V33A Cas2

Plasmid

#80093

Purpose

Encodes Cas1 V33A and Cas2

Depositor

George Church

Publication

Shipman et al Science. 2016 Jun 9. pii: aaf1175.

Inserts

Cas1 (cas1 )
Cas2 (cas2 )
CRISPR Array

Expression

Bacterial

Mutation

V33A

Availability

Academic Institutions and Nonprofits only

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