We narrowed to 10,183 results for: yeast

1. pEGFP-C1-hApg5

   Plasmid

   #22952

   Depositor

   Noboru Mizushima

   Article

   Mizushima et al J Biol Chem. 1998 Dec 18. 273(51):33889-

   Insert

   Homo sapiens ATG5 autophagy related 5 homolog (S. cerevisiae) (ATG5 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   July 14, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGTQL1211YN

   Plasmid

   #61704

   Purpose

   Gateway compatible BiFC vector (pEarleyGate201-YN)

   Depositor

   Yuhai Cui

   Article

   Lu et al Plant J. 2010 Jan;61(2):259-70. doi: 10.

   Type

   Empty backbone

   Use

   Bifc assay

   Tags

   YFP-N and HA

   Expression

   Plant

   Promoter

   35S

   Available Since

   May 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGTQL1221YC

   Plasmid

   #61705

   Purpose

   Gateway compatible BiFC vector (pEarleyGate202-YC)

   Depositor

   Yuhai Cui

   Article

   Lu et al Plant J. 2010 Jan;61(2):259-70. doi: 10.

   Type

   Empty backbone

   Use

   Bifc assay

   Tags

   YFP-C and FLAG

   Expression

   Plant

   Promoter

   35S

   Available Since

   May 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pV1524

   Plasmid

   #111431

   Purpose

   CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - inserts at Neut5L - Recyclable for serial mutagenesis (Mal2-FLP)

   Depositor

   Gerald Fink

   Article

   Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

   Insert

   CaCas9/sgRNA-BsmBI stuffer
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pS1643 (pFA6-NATMX-CDC42pr-yGFP1-10)

   Plasmid

   #86420

   Purpose

   N-terminal PCR tagging cassette for yeast codon optimized split GFP1-10 with CDC42 promoter

   Depositor

   Sue Jaspersen

   Article

   Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

   Insert

   CDC42pr-GFP1-10
   

   Tags

   GFP1-10

   Expression

   Yeast

   Available Since

   Feb. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJK-caCas9-NatMX-Neut5L

   Plasmid

   #89576

   Purpose

   caCas9 integrating vector into the Neut5L locus

   Depositor

   George Church

   Article

   Shapiro et al Nat Microbiol. 2018 Jan;3(1):73-82. doi:

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Yeast

   Available Since

   Oct. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-FLAG-Rheb-N153T

   Plasmid

   #19997

   Depositor

   Fuyuhiko Tamanoi

   Article

   Urano et al Proc Natl Acad Sci U S A. 2007 Feb 27. 1

   Insert

   Ras homolog enriched in brain 1 - N153T (RHEB Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Changed Asparagine 153 to Threonine

   Available Since

   Jan. 28, 2009

   Availability

   Academic Institutions and Nonprofits only

8. pYES260-sc.eEF2

   Plasmid

   #85119

   Purpose

   yeast expression of EEF2

   Depositor

   Pal Falnes

   Article

   Davydova et al J Biol Chem. 2014 Oct 31;289(44):30499-5

   Insert

   EFT1/EFT2 (EFT2, EFT1 Budding Yeast)
   

   Tags

   6xHis

   Expression

   Yeast

   Promoter

   GAL1

   Available Since

   Nov. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pYES260-sc.eEF2 K509A

   Plasmid

   #85120

   Purpose

   yeast expression of mutant EEF2

   Depositor

   Pal Falnes

   Article

   Davydova et al J Biol Chem. 2014 Oct 31;289(44):30499-5

   Insert

   EFT1/EFT2 (EFT2, EFT1 Budding Yeast)
   

   Tags

   6xHis

   Expression

   Yeast

   Mutation

   K509A

   Promoter

   GAL1

   Available Since

   Nov. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pV1393

    Plasmid

    #111430

    Purpose

    CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - inserts at Neut5L - Recyclable for serial mutagenesis (Sap2-FLP)

    Depositor

    Gerald Fink

    Article

    Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

    Insert

    CaCas9/sgRNA-BsmBI stuffer
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pfa6a PhoCl-TsCC(B)::KANMX

    Plasmid

    #185906

    Purpose

    Plasmid for cloning PhoCl fused to TsCC(B) into yeast.

    Depositor

    Matthew Good

    Article

    Garabedian et al Nat Chem Biol. 2021 Sep;17(9):998-1007.

    Insert

    PhoCl-TsCC(B)
    

    Tags

    PhoCl fluorophore with C-terminal TsCC(B)

    Expression

    Yeast

    Promoter

    SP6

    Available Since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. p415 TEF roGFP2‐Tsa2ΔCR

    Plasmid

    #83238

    Purpose

    The genetically encoded fluorescent H2O2 sensor roGFP2-Tsa2ΔCR cloned in the yeast p415 vector under the control of a TEF promoter. For cytosolic expression.

    Depositor

    Tobias Dick

    Article

    Morgan et al Nat Chem Biol. 2016 Jun;12(6):437-43. do

    Insert

    roGFP2-Tsa2dCr
    

    Tags

    roGFP2 fusion with Tsa2dCr

    Expression

    Yeast

    Promoter

    TEF

    Available Since

    Sept. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET26b_Nb.b201

    Plasmid

    #131404

    Purpose

    E. coli periplasmic expression of nanobody Nb.b201 recognizing human serum albumin

    Depositor

    Andrew Kruse

    Article

    McMahon et al Nat Struct Mol Biol. 2018 Mar;25(3):289-

    Insert

    Nanobody b201
    

    Tags

    6xHis-tag and pelB leader

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSJ1256 (pFA6-link-yGFP1-10-CaURA3MX)

    Plasmid

    #86419

    Purpose

    C-terminal PCR tagging module for yeast codon optimized split GFP1-10

    Depositor

    Sue Jaspersen

    Article

    Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

    Insert

    GFP1-10
    

    Expression

    Yeast

    Available Since

    Feb. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTP-PCR C3

    Plasmid

    #189620

    Purpose

    Cloned Reduced Mitochondrial Genome of the diatom Thalassiosira pseudonana in yeast and bacteria

    Depositor

    Bogumil Karas

    Article

    Cochrane et al Biology (Basel). 2020 Oct 26;9(11). pii:

    Insert

    Reduced Mitochondrial Genome of Thalassiosira pseudonana
    

    Use

    Synthetic Biology; Diatom expression

    Expression

    Bacterial and Yeast

    Mutation

    Removed repeat region, 10-kbp deletion, and minor…

    Available Since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pfa6a mScarlet-TsCC(B)::KANMX

    Plasmid

    #176397

    Purpose

    Plasmid for cloning mScarlet fused to TsCC(B) coiled coil into yeast.

    Depositor

    Matthew Good

    Article

    Garabedian et al Nat Chem Biol. 2021 Sep;17(9):998-1007.

    Insert

    mScarlet-TsCC(B)
    

    Tags

    mScarlet fused to TsCC(B)

    Expression

    Bacterial and Yeast

    Promoter

    none

    Available Since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCRISPEY-GAL-Kan-ADE2

    Plasmid

    #232105

    Purpose

    Expresses galactose-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

    Insert

    ADE2 gRNA and repair template
    

    Use

    CRISPR

    Expression

    Yeast

    Mutation

    Repair template introduces C at nt 466 of ADE2 to…

    Promoter

    GAL7

    Available Since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pG2J

    Plasmid

    #102882

    Purpose

    Expresses OsTIR1 via the ADH1 promoter, and the mGFP-AID via the TEF1 promoter in yeast. Both cassettes are integrated in HO locus. Assembly and characterization of the auxin-inducible degradation.

    Depositor

    Matthias Heinemann

    Article

    Papagiannakis et al Mol Cell. 2017 Jan 19;65(2):285-295. doi

    Inserts

    OsTIR1
    mGFP-AID
    

    Tags

    AID(71-114)

    Expression

    Yeast

    Promoter

    ADH1 and TEF1

    Available Since

    Jan. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. p415 TEF roGFP2‐Tsa2ΔCPΔCR

    Plasmid

    #83239

    Purpose

    The catalytically inactive genetically encoded fluorescent H2O2 sensor roGFP2-Tsa2ΔCPΔCR cloned in the yeast p415 vector under the control of a TEF promoter. For cytosolic expression.

    Depositor

    Tobias Dick

    Article

    Morgan et al Nat Chem Biol. 2016 Jun;12(6):437-43. do

    Insert

    roGFP2-Tsa2dCpdCr
    

    Tags

    roGFP2 fusion with Tsa2dCpdCr

    Expression

    Yeast

    Promoter

    TEF

    Available Since

    Sept. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. p403 – TDH3-MTS-mCherry-GFP1-10-His

    Plasmid

    #177730

    Purpose

    mCherry and the 1-10th β-strands of GFP (GFP1-10) fused to a mitochondria signal sequence for mitochondrial targeting in yeast plasmid with HIS marker

    Depositor

    Erik Böttger

    Article

    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

    Insert

    mCherry-GFP1-10
    

    Tags

    Mitochondrial Targeting Sequence

    Expression

    Bacterial and Yeast

    Available Since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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