We narrowed to 4,830 results for: Drosophila

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pGP-20XUAS-IVS-Syn21-op1-jGCaMP7b-T2A-tdTomato-p10

Plasmid

#172913

Purpose

Gal4 activated transgene coexpression of protein calcium sensor and tdTomato under the hsp70 promoter in D. melanogaster

Depositor

GENIE Project

Insert

jGCaMP7b-T2A-tdTomato

Tags

6xHis and T2A-tdTomato

Expression

Insect

Promoter

hsp70

Available since

Aug. 26, 2021

Availability

Academic Institutions and Nonprofits only

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BicC.Ca9.R

Plasmid

#168295

Purpose

Expresses Cas9 in Drosophila late germline cells

Depositor

Omar Akbari

Publication

Kandul et al CRISPR J. 2021 Oct;4(5):739-751. doi: 10

Insert

BicC.Cas9-T2A-eGFP-p10

Use

CRISPR

Expression

Insect

Available since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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pUBC_stdMCP_serinemod_E488QADAR_p2A_yGFP

Plasmid

#154787

Purpose

Expresses synonymized MS2 coat protein dimer (MCP) fused to hyperactive ADAR catalytic domain

Depositor

Robert Singer

Publication

Biswas et al iScience. 2020 Jun 28;23(7):101318. doi:

Insert

Adenosine deaminse (Adar Synthetic, Fly)

Use

Lentiviral

Tags

GFP, V5, and stdMCP

Expression

Mammalian

Mutation

Synonymized serine to prevent autoediting of ADAR…

Promoter

UBC

Available since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pDEST-APIC-Flp1

Plasmid

#112806

Purpose

gateway cloning destination vector to express enhancer driven Flip1 in Drosophila

Depositor

Chun Han

Publication

Poe et al Proc Natl Acad Sci U S A. 2017 Sep 19;11

Type

Empty backbone

Expression

Bacterial

Available since

Sept. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pRmHA3-CH2F2-CG9866

Plasmid

#52286

Purpose

Expresses Drosophila Ataxin-7

Depositor

Jerry Workman

Publication

Mohan et al Genes Dev. 2014 Feb 1;28(3):259-72. doi:

Insert

Ataxin-7 (CG9866 Fly)

Tags

2xHA 2xFLAG

Expression

Insect

Available since

April 24, 2014

Availability

Academic Institutions and Nonprofits only

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pCasperAUG-Gal4-X

Plasmid

#8378

Depositor

Leslie Vosshall

Publication

Vosshall et al Cell 2000 Jul 21;102(2):147-59.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Yeast Gal4 (GAL4 Budding Yeast, fused to first ~30 amino acids of Drosophila ADH)

Use

Drosophila p-element vector

Available since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pDmHsp70b

Plasmid

#165785

Purpose

Drosophila HSP70B promoter fragment, shows basal expression that can be further enhanced via heatshock

Depositor

Koen Venken

Publication

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Hsp70b

Expression

Insect

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pMCh-139

Plasmid

#92287

Purpose

Plasmid for expression of NHA-tagged Drosophila melanogaster GW182 NED in D. melanogaster cells

Depositor

Marina Chekulaeva, Witold Filipowicz

Publication

Chekulaeva et al Nucleic Acids Res. 2010 Oct;38(19):6673-

Insert

dGW182 NED (gw Fly)

Tags

NHA

Expression

Insect

Mutation

dGW182 NED: aa 205-490

Promoter

Ac5

Available since

Oct. 2, 2017

Availability

Academic Institutions and Nonprofits only

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LexAop2->Bxb1.STOP>myr::4xSNAPf

Plasmid

#87640

Purpose

For creating drosophila transgenics expressing LexAop2->Bxb1.STOP>myr::4xSNAPf

Depositor

Gregory Jefferis

Publication

Sutcliffe et al Genetics. 2017 Apr;205(4):1399-1408. doi

Insert

myr::4xSNAPf

Expression

Insect

Available since

March 24, 2017

Availability

Academic Institutions and Nonprofits only

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pAc5.1C-FLuc-Stop-5BoxB

Plasmid

#21301

Depositor

Elisa Izaurralde

Publication

Rehwinkel et al RNA. 2005 Nov . 11(11):1640-7.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

5BoxB

Use

Drosophila expression

Available since

June 23, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pRVV601

Plasmid

#87632

Purpose

RMCE Bxb1 attB-flanked vector.

Depositor

Richard Mann

Publication

Voutev et al Biotechniques. 2017 Jan 1;62(1):37-38. d

Insert

Bxb1 attB

Use

Rmce

Available since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Ac5-STABLE2-RFP-NLS-CycB(1-266)_GFP-E2F1(1-230)_neo

Plasmid

#73164

Purpose

multi-cistronic Vector for expression for Fly_FUCCI probes in insect cells

Depositor

Bruce Edgar

Publication

Zielke et al Cell Rep. 2014 Apr 24;7(2):588-98. doi:

Insert

mRFP-CycB(1-266)-T2A-GFP-E2F1(1-230)-T2A-neoR (E2f1, CycB Fly)

Tags

mRFP1, GFP

Expression

Insect

Mutation

Cyclin B Fragment aa 1-266 & E2F1 Fragment aa…

Available since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBS-KS-attB1-2-PT-SA-SD-1-2xTY1-V5

Plasmid

#61256

Purpose

CRISPR-RMCE, second step attB plasmid, intron phase 1, exchange dsRed with 2xTY1 and V5 tags cassette

Depositor

Frank Schnorrer

Publication

Zhang et al G3 (Bethesda). 2014 Oct 15. pii: g3.114.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

2xTY1-V5

Use

CRISPR

Tags

TY1 and V5

Available since

Nov. 20, 2014

Availability

Academic Institutions and Nonprofits only

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FLAG-MED6

Plasmid

#15411

Depositor

Joan Conaway, Ronald Conaway

Publication

Sato et al J Biol Chem. 2003 Dec 12. 278(50):49671-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

MED6 (Med6 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

N/A

Available since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pJFRC-20X-UAS-ADARcd

Plasmid

#81173

Purpose

identification of cell-specific targets of RNA binding proteins

Depositor

Michael Rosbash

Publication

McMahon et al Cell. 2016 Mar 30. pii: S0092-8674(16)30

Insert

UAS-ADARcd

Expression

Insect

Promoter

UAS

Available since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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UAS-FRT-GFP-FRT-u(M)Cas9

Plasmid

#127388

Purpose

Cas9 inducible by removal of a GFP flip-out cassette

Depositor

Michael Boutros

Publication

Port et al Elife. 2020 Feb 13;9. pii: 53865. doi: 1

Insert

FRT-GFP-FRT-uORF(M)Cas9

Use

CRISPR

Expression

Insect

Available since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

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UAS-u(S)Cas9

Plasmid

#127383

Purpose

Expresses Cas9 at high level

Depositor

Michael Boutros

Publication

Port et al Elife. 2020 Feb 13;9. pii: 53865. doi: 1

Insert

uORF(S)-Cas9

Use

CRISPR

Expression

Insect

Promoter

UAST

Available since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAc5.1C-RLuc-V5His6

Plasmid

#21182

Depositor

Elisa Izaurralde

Publication

Rehwinkel et al RNA. 2005 Nov . 11(11):1640-7.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Renilla Luciferase

Use

Drosophila expression

Available since

July 1, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCRISPaint-sfGFP-3xP3-RFP

Plasmid

#127566

Purpose

CRISPaint universal donor plasmid for gene exon insertion. Encodes sfGFP-SV40 and 3xP3-RFP visible eye marker.

Depositor

Norbert Perrimon

Publication

Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

Insert

sfGFP

Use

CRISPR

Expression

Insect

Available since

July 13, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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T7-phiC31-nos3'UTR

Plasmid

#182192

Purpose

in vitro transcription of phiC31 integrase mRNA

Depositor

David Stern

Publication

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

phiC31 integrase

Use

In vitro transcription

Available since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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