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Plasmid#218711PurposeExpresses N-terminally EGFP-tagged BBS1 in mammalian cellsDepositorInsertBBS1 (BBS1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSB1C3-FeGFP
Plasmid#138261PurposeDonor-eGFP vector to be used in plasmid to plasmid integration reporter assay. eGFP is conditionally expressed when integrated at iCas9-sites on acceptor plasmidDepositorInserteGFP
UseCRISPRTagsExpressionMammalianMutationPromoterNoneAvailable sinceMarch 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC19-link-sfGFP-FLAG:Kan
Plasmid#211833PurposePlasmid template that encodes the fluorescent protein sfGFP with a FLAG tag next to kanamycin resistance gene. This template was used to copy out sfGFP-FLAG-Kan for genetic tagging.DepositorInsertsfGFP-FLAG-Kan
UseTagsExpressionBacterialMutationNAPromoterAvailable sinceJan. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC19-link-PAGFP-FLAG:Kan
Plasmid#211834PurposePlasmid template that encodes the fluorescent protein PAGFP with a FLAG tag next to kanamycin resistance gene. This template was used to copy out PAGFP-FLAG-Kan for genetic tagging.DepositorInsertPAGFP-FLAG-Kan
UseTagsExpressionBacterialMutationNAPromoterAvailable sinceJan. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGB3_alpha1 P35Sx2_GFP_Tnos (GB2980)
Plasmid#160634PurposeTU for wtGFP expression driven by 2x35s promoter.DepositorInsertGFP
UseSynthetic BiologyTagsExpressionPlantMutationBsaI and BsmBI sites removedPromoter2x35sAvailable sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
NET29b.Mm pEGFP-N2 (1327)
Plasmid#62046Purposemammalian expression of nuclear envelope transmembrane proteinDepositorInsertNET29b (Tmem120b Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmSquash_B
Plasmid#145926PurposeInsect Expression of DmSquashDepositorInsertDmSquash (squ Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
NoLS-Grx1-roGFP2
Plasmid#236545PurposeExpressing fluorescent Grx1-roGFP2 fusion protein in mammalian cells with localization in the nucleoli.DepositorInsertNoLS-Grx1-roGFP2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmYTHDFa_AB
Plasmid#148291PurposeInsect Expression of DmYTHDFaDepositorInsertDmYTHDFa (CG6422 Fly)
UseTagsExpressionInsectMutation1 silent mutations compared to the sequence given…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsSmaug2_Q
Plasmid#147329PurposeMammalian Expression of HsSmaug2DepositorInsertHsSmaug2 (SAMD4B Human)
UseTagsExpressionMammalianMutationone silent mutation compared to the seqeuence giv…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmRpL10_R
Plasmid#147348PurposeInsect Expression of DmRpl10DepositorInsertDmRpl10 (RpL10 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP-PS9
Plasmid#177944PurposeTransient mammalian expression of eGFP along with SARS-CoV-2 sequence 20080-21171 (PS9) within the 3'UTR. Resulting transcript is packaged into SARS-CoV-2 virus-like particles.DepositorInserteGFP (ORF1ab )
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
LifeAct-LSS-SGFP2-N1
Plasmid#112938PurposeVisualization of F-actinDepositorInsertLifeAct
UseTagsLSS-SGFP2ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTre-Tight-luciferase-GFP (467)
Plasmid#65491Purposenegative control for expression assaysDepositorInsertEGFP
UseLuciferaseTagsExpressionMammalianMutationPromotertight TRE promoterAvailable sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBac[3xP3-gTc'v;Tc'rps3P-Tc'H2Av_EGFP]
Plasmid#86454PurposeNuclear reporter (histone-tagged EGFP) construct with the Tribolium specific ribosomal protein subunit 3 promoter (driving ubiquitous expression); (Transformation plasmid; Black eye marker)DepositorInsert3xP3-gTc'v;Tc'rps3P-Tc'H2Av_EGFP
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-FLEX-splitTVA-EGFP
Plasmid#59332PurposeExpresses splitTVA-P2A-EGFPDepositorInsertsplitTVA950-P2A-EGFP
UseAAV and Cre/LoxTagsGFPExpressionMammalianMutationPromoterCAGAvailable sinceSept. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-synP-FLEX-EGFP-B19G
Plasmid#59333PurposeExpresses EGFP-P2A-B19GDepositorInsertEGFP-P2A-B19G
UseAAV and Cre/LoxTagsGFPExpressionMammalianMutationPromoterSynapsin-1Available sinceSept. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
KIF3A560GFP
Plasmid#129769PurposeExpresses mouse Kinesin-2 KIF3A head and neck linker, dimerized through kinesin-1 neck-coil and coil-1 to 560 and GFP taggedDepositorInsertKIF3A (Kif3a Mouse)
UseTagsGFP and His6ExpressionBacterialMutationTruncation at 359, fused to kinesin-1 starting Al…PromoterAvailable sinceNov. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV-DIO-TC66T-2A-EGFP-2A-oG
Plasmid#178429PurposeExpresses TVA(TC66T), EGFP, and optimized Rabies G (oG) protein in a FLEX cassetteDepositorInsertsTVA950 Glu66→Thr
Optimized G
EGFP
UseAAV and Cre/Lox; Adeno-associated virusTagsExpressionMutationPromoterhSynAvailable sinceFeb. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N3-FLuc-K21-Rluc_AH
Plasmid#148847PurposeMammalian Expression of K21-RlucDepositorInsertK21-Rluc
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only