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Plasmid#179619PurposeExpress DAXX D/E region in bacteriaDepositorInsertdeath domain associated protein (DAXX Human)
UseTagsGST, His, and TEVExpressionBacterialMutationamino acids 414-505 of DAXXPromoterAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFB-Flag-MyoD(QQQ)-E12
Plasmid#26003DepositorInsertMyoD(QQQ)-E12 Heterodimer Fusion (Myod1 Mouse)
UseBaculovirus transfer vectorTagsFlagExpressionMutationChanged Lysine 99 to Glutamine; Lysine 102 to Glu…PromoterAvailable sinceSept. 8, 2010AvailabilityAcademic Institutions and Nonprofits only -
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MBP-FUS_FL_12E
Plasmid#98652PurposeExpresses MBP-tagged full length phosphomimetic FUSDepositorInsertFUS (FUS Human)
UseTags6xHis-MBPExpressionBacterialMutation12 S/T to EPromoterAvailable sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
MBP-FUS_FL_6E
Plasmid#98655PurposeExpresses MBP-tagged full length phosphomimetic FUSDepositorInsertFUS (FUS Human)
UseTags6xHis-MBPExpressionBacterialMutation6 S/T to EPromoterAvailable sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA6.2EmGFP-DAXX
Plasmid#169726PurposeMammalian expression construct of GFP tagged full length DAXXDepositorInsertDAXX (DAXX Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-cDAXX
Plasmid#169729PurposeBacterial expression of N-terminally 6His tagged cDAXXDepositorInsertDAXX (DAXX Human)
UseTags6 HisExpressionBacterialMutationPromoterT7Available sinceJune 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
MBP-FUS_LC_12E
Plasmid#98654PurposeExpresses MBP-tagged phosphomimetic FUS AA 1-163DepositorInsertFUS (FUS Human)
UseTags6xHis-MBPExpressionBacterialMutation12 S/T to EPromoterAvailable sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA6.2EmGFP-cDAXX
Plasmid#169727PurposeMammalian expression construct of GFP tagged cDAXXDepositorInsertcDAXX (DAXX Human)
UseTagsGFPExpressionMammalianMutationComprises residues 495-740PromoterCMVAvailable sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1.H1-NP
Plasmid#134367PurposeNanoluc complementation assay. Expression of histamine receptor H1 fused at C termimus with Natural peptide (NP) of NanoLuc. Addition of the Flag epitope at N terminus of H1 receptor.DepositorInsertH1-NP (HRH1 Human)
UseTagsFlag and natural peptide of nanoluciferaseExpressionBacterial and MammalianMutationPromoterT7Available sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFP Kind2 S181E
Plasmid#105312PurposeTo study altered downstream functions of phospholylation-site mimetic kindlin2DepositorInsertKindlin2 (FERMT2 Human)
UseTagsEGFPExpressionMammalianMutationS181EPromoterAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
plentiCRISPR-v2 Puro CHKA_sg2
Plasmid#218523PurposesgRNA targeting human CHKADepositorInsertCHKA (CHKA Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_ARHGEF4-1/1
Plasmid#91414PurposeProtein expression and purification of human SH3 domain construct ARHGEF4-1/1DepositorInsertARHGEF4-1/1 (ARHGEF4 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP Kind2 S159/666E
Plasmid#105313PurposeTo study altered downstream functions of phospholylation-site mimetic kindlin2DepositorInsertKindlin2 (FERMT2 Human)
UseTagsEGFPExpressionMammalianMutationS159/666EPromoterAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP KInd2 S159E
Plasmid#105311PurposeTo study altered downstream functions of phospholylation-site mimetic kindlin2DepositorInsertKindlin2 (FERMT2 Human)
UseTagsEGFPExpressionMammalianMutationS159EPromoterAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCC324
Plasmid#110165PurposeminiMos vector expresses ERKKTR-mClover and mCherry-H2B in C. elegans VPCs; request with plasmids pCC335 and pCC336 for unphosphorylated and phosphomimetic controls (Contains Neomycin resistance)DepositorInsertERK-KTR-mClover-T2A-mCherry-H2B
UseMinimos transposonTagsmCherry and mCloverExpressionWormMutationR61K change made in ERK-KTR CDSPromoterlin-31 promoter/enhancerAvailable sinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pINDUCER20 MEF2C S222D
Plasmid#107234PurposeMEF2C phosphomimetic mutant serine to aspartic acidDepositorInsertMEF2C
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA6.2EmGFP-cDAXX-0SB
Plasmid#169728PurposeMammalian expression construct of GFP tagged cDAXX-0SBDepositorInsertcDAXX-0SB (DAXX Human)
UseTagsGFPExpressionMammalianMutationComprises residues 495-741 and has the major SB m…PromoterCMVAvailable sinceJune 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFUGW-shRIIα-RIIαS97E-IRES-EGFP
Plasmid#183458PurposeReplacement of endogenous rat PKA-RIIα subunits with the RIIα S97E variantDepositorInsertsgRNA and Prkar2a (Prkar2a Rat)
UseLentiviralTagsExpressionMutationshRNA-resistant mutations: C135>G, G138>A, …PromotershRNA: H1 / gene: ubiquitinAvailable sinceJune 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
p36-p37-p38-p40-pET-Duet-1-Rfc3-T76D
Plasmid#175051PurposeOverexpression of human Rfc2,Rfc3-3KtoA,Rfc4,Rfc5 in E.coliDepositorUseTagsExpressionBacterialMutationchanged Thr76 to Asp, phosphomimetic mutationPromoterT7 promoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only