Addgene

We narrowed to 1,261 results for: membrane gfp

Showing: 601 - 620 of 1261 results

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pLenCMV-PMMars1CyGFP

Plasmid

#78315

Purpose

Lentiviral transfer vector encoding Mars1Cy-GFP membrane-spanning protein

Depositor

Alan Waggoner

Article

Zhang et al J Clin Invest. 2015 Oct 1;125(10):3915-2

Insert

Mars1Cy-TMD-GFP

Use

Lentiviral

Tags

HA epitope

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 1, 2016

Availability

Academic Institutions and Nonprofits only

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myr-palm-mEYFP-(L)-mCherry2-(L)-mEGFP-(L)-mApple

Plasmid

#182854

Purpose

Hetero-tetrmer expression vector with plasma membrane localization signal

Depositor

Salvatore Chiantia

Article

Dunsing et al Elife. 2021 Sep 8;10. pii: 69687. doi: 1

Insert

myr-palm-mEYFP-mCherry2-mEGFP-mApple

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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Venus-Bik-delMBR-pEGFP-C1

Plasmid

#177432

Purpose

To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, with its membrane binding region (MBR) deleted (Bik(1-136)). Compare to full length Addgene #166737.

Depositor

David Andrews

Insert

Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)

Use

Tags

Venus

Expression

Mammalian

Mutation

Bik MBR removed (after position 136)

Promoter

CMV

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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His-GFP-Rim2

Plasmid

#170869

Purpose

Mammalian expression of His-GFP-Rim2.

Depositor

Justin Taraska

Article

Prasai et al Nat Commun. 2021 Jun 25;12(1):3970. doi:

Insert

Regulating synaptic membrane exocytosis protein 2 (Rim 2) (Rims2 Rat)

Use

Tags

(His)6 and EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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pHR_TRE_Ncad-P2A-GFPlig

Plasmid

#133806

Purpose

inducible expression of Ncad and membrane-tethered GFP in mammalian cells

Depositor

Wendell Lim

Article

Toda et al Science. 2018 Jul 13;361(6398):156-162.

Insert

cadherin 2 (CDH2) (Cdh2 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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slc1a3b:myrGFP-P2A-H2AmCherry

Plasmid

#170208

Purpose

Drives myrGFP-P2A-H2AmCherry expression with the slc1a3b promoter in astrocytes. The myrGFP labels the membrane and mCherry labels the nucleus

Depositor

Kelly Monk

Article

Chen et al Nat Neurosci. 2020 Oct;23(10):1297-1306.

Insert

slc1a3b (slc1a3b Zebrafish)

Use

Zebrafish

Tags

myrGFP-P2A-H2AmCherry

Expression

Mutation

Promoter

slc1a3b

Available since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA3.0_LynSNAP IRES EGFP

Plasmid

#136625

Purpose

Plasmid encoding outer inner membrane localization of SNAP-Tag and EGFP

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

Lyn11-SNAP-Tag (IRES EGFP)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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EGFP-CaaX(tH) in pCSDEST2

Plasmid

#140890

Purpose

Expression of GFP-CaaX (sequence from Hras) in mammalian cells.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

CaaX membrane targeting motif (H-ras)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA3.0_LynNanobody IRES EGFP

Plasmid

#136624

Purpose

Plasmid encoding outer inner membrane localization of the Enhancer nanobody GFP as a SNAP-Tag-fusion and EGFP

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

Lyn11-SNAP-Tag-EnhancerNb (IRES EGFP)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 27, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV.GfaABC1D.PI.Lck-GFP.SV40

Plasmid

#105598

Purpose

AAV expression of membrane bound EGFP from GfaABC1D promoter

Depositor

Baljit Khakh

Article

Shigetomi et al J Gen Physiol. 2013 Apr 15.

Has Service

AAV5

Insert

Lck-EGFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

GfaABC1D

Available since

March 5, 2018

Availability

Academic Institutions and Nonprofits only

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CIB81-pmGFP

Plasmid

#75366

Purpose

Expresses aa1-81 of Arabidopsis CIB1 fused to EGFP with CaaX membrane tag on end

Depositor

Chandra Tucker

Article

Taslimi et al Nat Chem Biol. 2016 Apr 11. doi: 10.1038

Insert

CIB1 (CIB1 Mustard Weed)

Use

Tags

prenylated EGFP

Expression

Mammalian

Mutation

residues 1-81 of CIB1

Promoter

CMV

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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PZac2.1 hSyn1-Lck-GFP

Plasmid

#177333

Purpose

Expresses GFP on the plasma membrane of neurons via an Lck localization sequence

Depositor

Baljit Khakh

Article

Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

Insert

Lck-GFP

Use

AAV

Tags

GFP

Expression

Bacterial

Mutation

Promoter

hSyn1

Available since

April 11, 2023

Availability

Academic Institutions and Nonprofits only

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pPyCAG-EGFP-TM-IRES-Pac

Plasmid

#183603

Purpose

Mammalian expression vector for membrane-tethered EGFP from the CAG promoter. Confers puromycin resistance.

Depositor

Sally Lowell

Article

Malaguti et al Development. 2022 May 26. pii: 275525. d

Insert

EGFP-TM

Use

Tags

Human PDGFRB transmembrane domain and Mouse IgGK …

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 LvWSCP-EGFP

Plasmid

#196480

Purpose

Mammalian expression of WSCP-EGFP

Depositor

Fei Sun

Article

Li et al Nat Commun. 2022 Jun 9;13(1):3197. doi:

Insert

LvWSCP-EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 27, 2023

Availability

Academic Institutions and Nonprofits only

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Stargazin-GFP-LOVpep

Plasmid

#80406

Purpose

Membrane localized GFP-tagged LOVpep. Expresses in mammalian cells

Depositor

Michael Glotzer

Article

Wagner et al J Cell Biol. 2016 Jun 20;213(6):641-9. d

Insert

Stargazin-GFP-LOVpep (CACNG2 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 1, 2016

Availability

Academic Institutions and Nonprofits only

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Lyn11-CIBN-GFP

Plasmid

#79572

Purpose

expression of plasma membrane signal from Lyn11 fused to CIBN and tagged with GFP

Depositor

Pietro De Camilli, Olof Idevall-Hagren

Article

Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

Insert

Lyn11

Use

Tags

CIBN (CRY2-binding domain) and EGFP

Expression

Mammalian

Mutation

G8R (please see depositor comments below)

Promoter

CMV

Available since

July 29, 2016

Availability

Academic Institutions and Nonprofits only
pCIB1(deltaNLS)-pmGFP

Plasmid

#28240

Purpose

Expresses CIB1 fused to plasma membrane-localized EGFP

Depositor

Chandra Tucker

Article

Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

Insert

CIB1(deltaNLS)-pmGFP (CIB1 Mustard Weed)

Use

Tags

EGFP

Expression

Mammalian

Mutation

NLS sequences mutated

Promoter

Available since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

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pT2-CAG-fGFP

Plasmid

#108714

Purpose

Transposon donor plasmid for long-term expression of membrane targetted GFP

Depositor

Madeline Lancaster

Article

Lancaster et al Nat Biotechnol. 2017 Jul;35(7):659-666.

Insert

EGFP

Use

Tags

6xHis and farnesylation signal

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 10, 2018

Availability

Academic Institutions and Nonprofits only

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pISceI Dusp6:memGFP

Plasmid

#32668

Purpose

Expresses membrane localized EGFP under the Dusp6 promoter

Depositor

Michael Tsang

Article

Wang et al Development. 2011 Jan;138(1):45-54. Epub

Insert

Dusp6 promoter (dusp6 Zebrafish)

Use

Zebrafish transgenesis

Tags

Expression

Mutation

Promoter

Available since

Nov. 28, 2011

Availability

Academic Institutions and Nonprofits only

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pEGFP VAMP4

Plasmid

#42313

Depositor

Thierry Galli

Article

Mallard et al J Cell Biol. 2002 Feb 18;156(4):653-64.

Insert

VAMP 4 (VAMP4 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 1, 2013

Availability

Academic Institutions and Nonprofits only

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