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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 619 results for: prp

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Showing: 601 - 619 of 619 results

  1. sgSf3b1(T1)

    Plasmid
    #90424
    Purpose
    induces dsDNA break within mouse Sf3b1 gene for subsequent homology-directed recombination and coding sequence mutagenesis at codon 700
    Depositor
    Alex Minella
    Article
    Gupta et al Nucleic Acids Res. 2018 Nov 20. pii: 519
    Insert
    mus Sf3b1 gRNA (Sf3b1 Mouse)
    Use
    CRISPR; Guiderna encoding for gene editing
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    90424-plasmid-map-sequence-id-190772

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  2. pL452(hygro)-Sf3b1-K700K

    Plasmid
    #90426
    Purpose
    selectable HDR vector to introduce silent mutation (position 700) in mus Sf3b1 gene (for use with pL452-Sf3b1-K700E and sgSf3b1(T1) gRNA vectors enabling generation of hemizygous K700E mutation)
    Depositor
    Alex Minella
    Article
    Gupta et al Nucleic Acids Res. 2018 Nov 20. pii: 519
    Insert
    Sf3b1 - left and right homology arms (Sf3b1 Mouse)
    Use
    Homology-directed repair vector
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    90426-plasmid-map-sequence-id-190769

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  8. pPICZ A-SLC[∆2-83]-His+MFN2-Strep (SB259)

    Plasmid
    #227608
    Purpose
    Inducible coexpression of His-tagged SLC25A46 in its N-terminal region (∆2-83) and Strep-tagged MFN2 in Pichia pastoris
    Depositor
    Luke Chao
    Article
    Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10
    Inserts
    SLC25A46 (SLC25A46 Human)
    MFN2 (MFN2 Human)
    Tags
    10xHis and Twin-StrepTag
    Expression
    Yeast
    Mutation
    aa 2-83 deleted
    Promoter
    AOX1
    Available Since
    Dec. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    227608-plasmid-map-sequence-id-455573

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  12. pPICZ A-SLC-His+MFN2[∆TM]-Strep (SB260)

    Plasmid
    #227609
    Purpose
    Inducible coexpression of His-tagged SLC25A46 and Strep-tagged MFN2 lacking its transmembrane region in Pichia pastoris
    Depositor
    Luke Chao
    Article
    Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10
    Inserts
    SLC25A46 (SLC25A46 Human)
    MFN2 (MFN2 Human)
    Tags
    10xHis and Twin-StrepTag
    Expression
    Yeast
    Mutation
    contains aa 2-544, 651-757 only [TM deleted]
    Promoter
    AOX1
    Available Since
    Dec. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    227609-plasmid-map-sequence-id-455572

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  13. pPICZ A-SLC(2-88)-MBP-His+MFN2-Strep (SB262)

    Plasmid
    #227611
    Purpose
    Inducible coexpression of His-tagged SLC25A46 truncated to its N-terminal region (2-88) fused to MBP and MFN2-Strep in Pichia pastoris
    Depositor
    Luke Chao
    Article
    Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10
    Inserts
    SLC25A46 (SLC25A46 Human)
    MFN2 (MFN2 Human)
    Tags
    MBP, 10xHis and Twin-StrepTag
    Expression
    Yeast
    Mutation
    contains aa 2-88 only
    Promoter
    AOX1
    Available Since
    Dec. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    227611-plasmid-map-sequence-id-455570

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  14. pPICZ A-SLC-His+MFN2[∆TM,∆HB2]-Strep (SB261)

    Plasmid
    #227610
    Purpose
    Inducible coexpression of His-tagged SLC25A46 and Strep-tagged MFN2 lacking its transmembrane region and helical bundle 2 (HB2) in Pichia pastoris
    Depositor
    Luke Chao
    Article
    Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10
    Inserts
    SLC25A46 (SLC25A46 Human)
    MFN2 (MFN2 Human)
    Tags
    10xHis and Twin-StrepTag
    Expression
    Yeast
    Mutation
    contains aa 2-400, 706-757 [TM and HB2 deleted]
    Promoter
    AOX1
    Available Since
    Dec. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    227610-plasmid-map-sequence-id-455569

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  15. POMBOX MoClo YTK

    Plasmid Kit
    #1000000251
    Purpose
    Seamless and efficient assembly of single gene and multigene plasmids for genome integration in Schizosaccharomyces pombe.
    Depositor
    Tomáš Pluskal
    Application
    Cloning and Synthetic Biology
    Vector Type
    Yeast Expression
    Cloning Type
    Golden Gate (MoClo)
    Available Since
    Jan. 23, 2025
    Availability
    Academic Institutions and Nonprofits only

  16. GoldenPiCS Kit

    Plasmid Kit
    #1000000133
    Purpose
    A flexible modular system for advanced strain engineering in Pichia pastoris to accomplish pathway expression, protein production, or other DNA integration applications.
    Depositor
    Brigitte Gasser
    Application
    Cloning and Synthetic Biology, Gene Expression and Labeling
    Vector Type
    Yeast Expression
    Cloning Type
    Golden Gate
    Available Since
    Feb. 20, 2018
    Availability
    Academic Institutions and Nonprofits only

  17. CCSB-Broad Human Kinase ORF Collection

    Plasmid Kit
    #1000000014
    Purpose
    Plasmids for over 500 human kinases and kinase-related proteins in Gateway entry vectors.
    Depositor
    William Hahn, David Root
    Application
    Gene Expression and Labeling
    Vector Type
    Mammalian Expression
    Available Since
    July 16, 2011
    Availability
    Academic Institutions and Nonprofits only

  18. ScEnSor Kit

    Plasmid Kit
    #1000000215
    Purpose
    To investigate the intracellular environment of Saccharomyces cerevisiae with a variety of biosensors.
    Depositor
    Lisbeth Olsson
    Application
    Gene Expression and Labeling
    Vector Type
    Yeast Expression
    Available Since
    May 8, 2023
    Availability
    Academic Institutions and Nonprofits only

  19. MoChlo: Modular Cloning Chloroplast Toolbox

    Plasmid Kit
    #1000000156
    Purpose
    Library of standardized chloroplast-specific genetic modules for Golden-Gate cloning of synthetic operons into chloroplast transformation vectors.
    Depositor
    Scott Lenaghan
    Application
    Cloning and Synthetic Biology
    Vector Type
    Plant Expression
    Cloning Type
    Golden Gate (MoClo)
    Available Since
    Aug. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
Showing: 601 - 619 of 619 results