We narrowed to 60,290 results for: cloning
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Plasmid#50271DepositorInsertpromoter, nos, (Agrobacterium tumefaciens) + 5'UTR, omega (Tobacco Mosaic Virus)
UsePlant expressionMutationBsaI/BbsI sites removed by point-mutationAvailable SinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMINTC3GH (Apr)
Plasmid#110078PurposeAnalogous to pMINTNH3 but with a C-terminal 3C cleavage site and a GFP-His10 tag fusion.DepositorTypeEmpty backboneTags3C cleavage site - GFP - His10 TagExpressionBacterialAvailable SinceMay 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMC-Ura-Cen-lvl1
Plasmid#176182Purposepreassembled yeast MoClo level-1 backbone with GFP dropout and URA3 markerDepositorTypeEmpty backboneExpressionYeastAvailable SinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMC-X2
Plasmid#176152Purposepreassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site X-2DepositorTypeEmpty backboneExpressionYeastAvailable SinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMC-X3
Plasmid#176153Purposepreassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site X-3DepositorTypeEmpty backboneExpressionYeastAvailable SinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMC-XI1
Plasmid#176155Purposepreassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XI-1DepositorTypeEmpty backboneExpressionYeastAvailable SinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMC-XI5
Plasmid#176158Purposepreassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XI-5DepositorTypeEmpty backboneExpressionYeastAvailable SinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMC-XII2
Plasmid#176160Purposepreassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XII-2DepositorTypeEmpty backboneExpressionYeastAvailable SinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
GGE020
Plasmid#120777PurposePart of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Terminator of gene expression for junction of position1-3. When using the GG platform for expressing only one gene (T1-3_Lip2)DepositorInsertT1-3_Lip2
UseCloning/assembly plasmidAvailable SinceNov. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMEXC3GH (Apr)
Plasmid#110083PurposeAnalogous to pMINTC3GH but containing oriM for episomal replication in mycobacteria.DepositorTypeEmpty backboneTags3C cleavage site - GFP - His10 TagExpressionBacterialAvailable SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDEST(frt)(ATG_V5-APEX2_noSTOP)
Plasmid#168207PurposeDestination vector for GATEWAY cloning, N-terminal V5-APEX2, Tet operator in the CMV promoter (Tet ON), FRT sequence for T-Rex stable expression, Hygromycin selectionDepositorTypeEmpty backboneUseUnspecified; Pdest gatewayTagsV5 APEX2ExpressionMammalianPromoterCMVAvailable SinceDec. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-miniTurbo-V5-DSTYK _IDG-K
Plasmid#158182PurposeGateway destination clone of DSTYK (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertminiTurbo-V5-DSTYK (DSTYK Human)
UseLentiviral; Gateway destinationTagsminiTurbo-V5ExpressionMammalianPromoterUbiquitinAvailable SinceOct. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUDP003
Plasmid#101164PurposeE. coli/S. cerevisiae amdS shuttle vector allowing cloning of ribozyme flanked g-RNA for Cas9 editing (HH-gRNA-HDV)DepositorTypeEmpty backboneUseCRISPRExpressionYeastPromoterScTDH3Available SinceDec. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pENTR-R4R3-3xmEGFP-C
Plasmid#113751PurposeGateway multi-site entry clone for second position 3x mEGFP (C-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.DepositorInsertmEGFP-mEGFP-mEGFP
UseCRISPR; Gateway entry vectorTags3xmEGFPAvailable SinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Kan_FR13_00004061
Plasmid#121292PurposeLevel 0 Golden-Gate compatible vectorDepositorInsertFR13_00004061
UseGolden gateMutationMagnaporthe oryzaeAvailable SinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Kan_GY11_00131271
Plasmid#121355PurposeLevel 0 Golden-Gate compatible vectorDepositorInsertGY11_00131271
UseGolden gateMutationMagnaporthe oryzaeAvailable SinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJOG387
Plasmid#105338PurposeSynthetic biology; gateway destination vector assemblyDepositorTypeEmpty backboneUseSynthetic BiologyAvailable SinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDYSCKO
Plasmid#120263PurposeEmpty backbone for cloning any gRNA to be used with the canavanine co-selection systemDepositorInsertDYSCKO cassette
UseCRISPR and Synthetic BiologyPromoterSNR52Available SinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJOG545
Plasmid#105424PurposeSynthetic biologyDepositorInsertAvrBs3
UseSynthetic BiologyMutationBsaI/ BpiI restriction sites removedAvailable SinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR-R4R3-3xmRuby-N
Plasmid#113754PurposeGateway multi-site entry clone for second position 3x mRuby (N-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.DepositorInsertmRuby2-mRuby2-mRuby2
UseCRISPR; Gateway entry vectorTags3xmRuby2Available SinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only