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pDONR223_RB1_p.L550I

Plasmid

#81596

Purpose

Gateway Donor vector containing RB1 ,part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

RB1 (RB1 Human)

Use

Gateway entry vector

Mutation

L550I

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_RB1_p.V654L

Plasmid

#81562

Purpose

Gateway Donor vector containing RB1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

RB1 (RB1 Human)

Use

Gateway entry vector

Mutation

V654L

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_RB1_p.F755I

Plasmid

#81546

Purpose

Gateway Donor vector containing RB1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

RB1 (RB1 Human)

Use

Gateway entry vector

Mutation

F755I

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_CHD2_p.P474R

Plasmid

#81474

Purpose

Gateway Donor vector containing CHD2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CHD2 (CHD2 Human)

Use

Gateway entry vector

Mutation

P474R

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_IRF4_p.R96L

Plasmid

#81435

Purpose

Gateway Donor vector containing IRF4 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

IRF4 (IRF4 Human)

Use

Gateway entry vector

Mutation

R96L

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223-LOC442075

Plasmid

#23372

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

LOC442075 (LOC442075 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-LOC390877

Plasmid

#23373

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

LOC390877 (LOC390877 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-PANK3

Plasmid

#23384

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PANK3 (PANK3 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-LOC649288

Plasmid

#23391

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

LOC649288 (AK4P6 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-LOC648152

Plasmid

#23513

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

LOC648152 (LOC648152 Human)

Use

Gateway donor vector

Mutation

1MM

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-PSKH1

Plasmid

#23514

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PSKH1 (PSKH1 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-MORN2

Plasmid

#23547

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MORN2 (MORN2 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-TSSK6

Plasmid

#23775

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

TSSK6 (TSSK6 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
Jacquere (all-in-one vector)

Pooled Library

#247027

Purpose

Human genome-wide CRISPR knockout library for the use of S. pyogenes Cas9. Each gene is targeted by 3 constructs.

Depositor

John Doench, David Root

Article

Drepanos et al bioRxiv 2025.08.26.672375; doi: https://

Expression

Mammalian

Species

Homo sapiens

Use

CRISPR and Lentiviral

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only
Julianna (split-vector)

Pooled Library

#247028

Purpose

Enriched for effective guides through prioritization of guides with strong on-target activity. Designed against current gene annotations, offering coverage of the contemporary mouse genome.

Depositor

John Doench, David Root

Article

Drepanos et al bioRxiv 2025.08.26.672375; doi: https://

Expression

Mammalian

Species

Mus musculus

Use

CRISPR and Lentiviral

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only
Mouse Cardiac Transcriptional Regulators CRISPR Knockout Library

Pooled Library

#138015

Purpose

AAV library targeting transcriptional regulators expressed in cardiomyocytes.

Depositor

William Pu

Article

VanDusen et al bioRxiv 2019, 808402v1; doi: https://doi

Species

Mus musculus

Use

AAV, CRISPR, and Cre/Lox

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only
pBD-PYR1-DSM_Hao_library

Pooled Library

#241289

Purpose

Library of pBD-PYR1 double site mutants for yeast-based screens; redesigned to remove constitutive mutants and wild type rceptors

Depositor

Sean Cutler

Article

Tian et al bioRxiv [Preprint]. 2025 May 15:2025.05.

Expression

Yeast

Species

Arabidopsis thaliana

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only
pDONR223-PDGFRB

Plasmid

#23893

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PDGFRB (PDGFRB Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pDONR223-ALK

Plasmid

#23917

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

ALK (ALK Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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RB1-TP53-LRG-GFP

Plasmid

#225876

Purpose

Guides targeting TP53 and RB1

Depositor

Janai Carr-Ascher

Article

Freeland et al Clin Cancer Res. 2024 Aug 23. doi: 10.11

Insert

RB1 and TP53 (TP53, RB1 Human)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge