We narrowed to 19,051 results for: Tec;

1. pcDNA3-blueCKAR(T/A)

   Plasmid

   #118473

   Purpose

   Negative-control mutant for blueCKAR biosensor.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   blueCKAR(T/A)
   

   Tags

   6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

   Expression

   Mammalian

   Mutation

   Contains Thr-to-Ala mutation in substrate sequenc…

   Promoter

   CMV

   Available Since

   Dec. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV-EF1A-LivePAR(R163A)-Hygro

   Plasmid

   #187609

   Purpose

   EGFP fused to the C-terminus of a WWE domain containing the mutation Arg163Ala & a hygromycin resistance cassette.

   Depositor

   Robert Sobol

   Article

   Koczor et al Cancers (Basel). 2022 Jul 28;14(15). pii

   Insert

   LivePAR(R163A)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   R163A mutation in the WWE Domain

   Promoter

   EF1A

   Available Since

   Sept. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCfB2899(X-2 MarkerFree)

   Plasmid

   #73271

   Purpose

   EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site X-2 (Chr X: 194944..195980)

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Expression

   Yeast

   Available Since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. mPlum-Gamma-Tubulin-17

   Plasmid

   #55971

   Purpose

   Localization: Centrosomes, Excitation: 590, Emission: 649

   Depositor

   Michael Davidson

   Article

   Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

   Insert

   Gamma-Tubulin (TUBG1 Human)
   

   Tags

   mPlum

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pCellFree_G03 ANXA1

   Plasmid

   #67071

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   ANXA1 (ANXA1 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

6. p3E-GBP

   Plasmid

   #67672

   Purpose

   Multisite gateway entry clone for adding C-terminal fusions of GFP-binding nanobody (GBP)

   Depositor

   Rob Parton

   Article

   Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

   Insert

   anti-GFP nanobody
   

   Use

   Gateway multisite 3' entry clone

   Available Since

   Nov. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. CDK4mut(K35M)-3XFlag-pBabe

   Plasmid

   #73559

   Purpose

   vector encoding a fusion protein between a CDK4 mutant (K35M) and 3 Flag tags

   Depositor

   Gerardo Ferbeyre

   Article

   Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d

   Insert

   CDK4mut(K35M)
   

   Use

   Retroviral

   Tags

   3X-FLAG

   Expression

   Mammalian

   Promoter

   SV40

   Available Since

   March 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLKO.1P shABCB7_2

   Plasmid

   #102969

   Purpose

   Suppression of ABCB7

   Depositor

   Richard Possemato

   Article

   Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

   Insert

   shABCB7_2 (ABCB7 Human)
   

   Use

   Lentiviral and RNAi

   Available Since

   Nov. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLKO.1P shABCB7_1

   Plasmid

   #102968

   Purpose

   Suppression of ABCB7

   Depositor

   Richard Possemato

   Article

   Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

   Insert

   shABCB7_1 (ABCB7 Human)
   

   Use

   Lentiviral and RNAi

   Available Since

   Nov. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET42b-A3A-PBE-ΔUGI

    Plasmid

    #119771

    Purpose

    To express the recombinant APOBEC3A in vitro

    Depositor

    Caixia Gao

    Article

    Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

    Insert

    APOBEC3A-nCas9-NLS
    

    Tags

    6*HIS and NLS

    Expression

    Bacterial

    Mutation

    nCas9 has a mutation D10A in wild type SpCas9

    Promoter

    T7 promoter

    Available Since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pVH268-Tier1-OTetO7-PCMVmin2-SEAP-p2A-iRFP670

    Plasmid

    #169591

    Purpose

    Tier-1 vector encoding PTetO7-driven SEAP-p2A-iRFP670 expression (OTetO7-PCMVmin-2-SEAP-p2A-iRFP670-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    tetracycline-controlled SEAP and iRFP expression
    

    Expression

    Mammalian

    Promoter

    TetO7-PCMVmin-2

    Available Since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. mEos2-Alpha-Actinin-19

    Plasmid

    #57346

    Purpose

    Localization: Alpha Actinin, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

    Insert

    Alpha-Actinin (ACTN1 Human)
    

    Tags

    mEos2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pVH617

    Plasmid

    #169606

    Purpose

    Tier-2 vector encoding PmPGK1-driven Fluc and PCREm4-driven Nluc expression (A1-pA::PmPGK1-Fluc-pA::PCREm4-Nluc-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PPGK-driven FLuc Luciferase expression cassette and cAMP response element-controlled NanoLuc Luciferase expression cassette
    

    Expression

    Mammalian

    Promoter

    PCREm4 / PPGK

    Available Since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. RS17060_pMQ30_KO_Kan

    Plasmid

    #185396

    Purpose

    Non-replicating vector used to create markerless deletion of RR42_RS17060 in C. basilensis 4G11

    Depositor

    Adam Arkin

    Article

    Sander et al Biotechnol Bioeng. 2024 Jan;121(1):139-1

    Inserts

    RR42_RS17060 upstream flanking homology region
    RR42_RS17060 downstream flanking homology region
    

    Available Since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. RS17055_pMQ30_KO_Kan

    Plasmid

    #185398

    Purpose

    Non-replicating vector used to create markerless deletion of RR42_RS17055 in C. basilensis 4G11

    Depositor

    Adam Arkin

    Article

    Sander et al Biotechnol Bioeng. 2024 Jan;121(1):139-1

    Inserts

    RR42_RS17055 upstream flanking homology region
    RR42_RS17055 downstream flanking homology region
    

    Available Since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCold I-Hero9

    Plasmid

    #187927

    Purpose

    Bacterial expression of heat-resistant obscure (Hero) protein Hero9

    Depositor

    Yukihide Tomari

    Article

    Tsuboyama et al PLoS Biol. 2020 Mar 12;18(3):e3000632. d

    Insert

    Hero9
    

    Tags

    Flag-6xHis

    Expression

    Bacterial

    Available Since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. LLP737_L2pV1_1I_Act2_FRT_OCS_Act2_Flp_lacZ

    Plasmid

    #192380

    Purpose

    To test if the Act2 promoter can drive the Flp recomhinase in order to remove the OCS terminator and increase circuit output.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-OCS-FRT::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Available Since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCellFree_G03 STAT3

    Plasmid

    #67120

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    STAT3 (STAT3 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pCellFree_G03 ISG15

    Plasmid

    #67054

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    ISG15 (ISG15 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

20. TAL2344

    Plasmid

    #36764

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    PTEN-TALEN_Left (Pten Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Promoter

    CMV

    Available Since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only