164,890 results

1. pcDNA3-Cyto-ExRai-CKAR2

   Plasmid

   #236097

   Purpose

   Cytosol-targeted enhanced excitation-ratiometric biosensor for monitoring Protein Kinase C activity in living cells.

   Depositor

   Jin Zhang

   Article

   Su et al Nat Chem Biol. 2024 Oct 11. doi: 10.1038

   Insert

   ExRai-CKAR2-NES
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLD-puro-Cc-SARS-CoV-2_NSP16-VA

   Plasmid

   #191215

   Purpose

   Lentiviral expression of SARS-CoV-2 NSP16 in mammalian cells

   Depositor

   Mohan Babu

   Insert

   Non structural protein 16 (ORF1ab SARS CoV-2)
   

   Use

   Lentiviral

   Tags

   VA tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTR01F/hFGFR2b-V5

   Plasmid

   #236014

   Purpose

   for PiggyBac mediated integration and stable expression of hFGFR2b protein

   Depositor

   Pavel Krejčí

   Article

   Zlinska et al Mol Ther Nucleic Acids. 2024 Nov 28;36(1

   Insert

   human FGFR2b (FGFR2 Human)
   

   Use

   Tags

   V5/His

   Expression

   Mammalian

   Mutation

   Promoter

   truncated CMV promoter

   Available since

   April 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJAK184.tetR.PT5-3.dCas9

   Plasmid

   #232482

   Purpose

   optimized ahTet inducible dCas9 for insertion into C. difficile genome

   Depositor

   James Collins

   Article

   Chua et al Microbiol Resour Announc. 2025 Apr 28:e0

   Insert

   Catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   TetR-PT5-3

   Available since

   April 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJC.15A.sgRNA.TA

   Plasmid

   #232483

   Purpose

   sgRNA cloning backbone for expression of sgRNA in C. difficile. Contains TA system to enable maintenence without antibiotic selection.

   Depositor

   James Collins

   Article

   Chua et al Microbiol Resour Announc. 2025 Apr 28:e0

   Inserts

   Toxin-antitoxin System from CD630
   medium-copy-number p15A origin of replication
   mCherry
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   P3 constitutive promoter and native promoter

   Available since

   April 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-PDGFRA-V5/HIS

   Plasmid

   #234754

   Purpose

   Expression of human PDGFRA receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   PDGFRA receptor tyrosine kinase, full length, wildtype (PDGFRA Human)
   

   Use

   Tags

   V5/His

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   CMV

   Available since

   April 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHAGE_FKBP-mEGFP-WIPI2d_P2A-FRB-Fis1 (no mt-mKeima)

   Plasmid

   #223794

   Purpose

   Stable expression of protein in mammalian cell culture

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

   Insert

   2xFKBP-mEGFP-WIPI1-(P2A)-FRB-Fis1 (WIPI1, FIS1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. AiP15646: pAAV-AiE1426m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN5646)

   Plasmid

   #230884

   Purpose

   AiE1426m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical and striatal Sst-Chodl neurons

   Depositor

   The Allen Institute for Brain Science, Jonathan Ting

   Article

   Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

   Insert

   SYFP2
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 29, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. AiP14202 - pAAV-AiE0743m_3xC2-minBG-mScarlet-WPRE3-BGHpA (Alias: CN4202)

   Plasmid

   #224178

   Purpose

   AiE0743m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal cholinergic neurons

   Depositor

   The Allen Institute for Brain Science, Jonathan Ting

   Article

   Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

   Insert

   mScarlet
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. AiP13808 - pAAV-AiE0140h_3xC2-minBG-mTFP1-WPRE3-BGHpA (Alias: CN3808)

    Plasmid

    #224176

    Purpose

    AiE0140h_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal Pvalb+ neurons (note cortical Pvalb+ and L5_ET)

    Depositor

    The Allen Institute for Brain Science, Jonathan Ting

    Article

    Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

    Insert

    mTFP1
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. p3E-WPRE-SV40-pA (JDW 922)

    Plasmid

    #224545

    Purpose

    A Gateway compatible 3' entry clone containing a woodchuck herpes simplex virus regulatory element (WPRE) to stabilize mRNA upstream of an SV40 polyA

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    WPRE-SV40-pA
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. Human USP7_H456A-3X FLAG

    Plasmid

    #225335

    Purpose

    Lentiviral expression of human mutant USP7 with 3x FLAG tag in mammalian cells

    Depositor

    Huda Zoghbi

    Article

    Kim et al Neuron. 2023 Mar 15;111(6):824-838.e7. d

    Insert

    USP7 (USP7 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    H456A

    Promoter

    EF1a

    Available since

    Sept. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. EGFP-Cyclin D2

    Plasmid

    #215051

    Purpose

    Expresses EGFP-Cyclin D2 in mammalian cells from a retroviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    CCND2 (CCND2 Human)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCW-LIC-N8C_Fab2b

    Plasmid

    #203692

    Purpose

    Expresses N8C_Fab2b

    Depositor

    Brenda Schulman

    Article

    Henneberg et al Nat Chem Biol. 2023 Dec;19(12):1513-1523

    Insert

    N8C_Fab2b
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PVALB mAb _BCN14.1.1C9_HC_Mouse IgG2a

    Plasmid

    #182888

    Purpose

    Mammalian expression of PVALB mouse mAb HC gene

    Depositor

    Heng Zhu

    Insert

    anti-PVALB (human) heavy chain (PVALB Mouse)
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Expression

    Mammalian

    Mutation

    No

    Promoter

    CMV

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pE5770

    Plasmid

    #170777

    Purpose

    expression of hybrid proteins in E. coli

    Depositor

    Anthony Schryvers

    Article

    Chaudhuri et al Methods Mol Biol. 2022;2414:115-140. doi

    Insert

    Kan resistance (kanR )
    

    Use

    Tags

    polyhisbioMbpTEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUC57-sgRNA-MS2-U6

    Plasmid

    #171694

    Purpose

    To construct expression vector for tBE-V5-mA3

    Depositor

    Jia Chen

    Article

    Wang et al Nat Cell Biol. 2021 May 10. pii: 10.1038

    Insert

    sgRNA scaffold-MS2-U6
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pU6-ccdB-boxB-tBE-V5-mA3

    Plasmid

    #171693

    Purpose

    Expresses tBE-V5-mA3 in mammalian cells

    Depositor

    Jia Chen

    Article

    Wang et al Nat Cell Biol. 2021 May 10. pii: 10.1038

    Insert

    ccdB-sgRNA scaffold-boxB-tBE-V5-mA3
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and EF1a

    Available since

    Aug. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. His-GFP-Clathrin

    Plasmid

    #170858

    Purpose

    Mammalian expression of His-GFP-Clathrin.

    Depositor

    Justin Taraska

    Article

    Prasai et al Nat Commun. 2021 Jun 25;12(1):3970. doi:

    Insert

    Clathrin light polypeptide (Lca) (Clta Mouse)
    

    Use

    Tags

    (His)6 and EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CMV-H2B-mScarlet-I

    Plasmid

    #182297

    Purpose

    Localization of mScarlet-I fluorescent protein to histone 2B

    Depositor

    Thierry Fournier, Severine Degrelle

    Article

    Degrelle et al Environ Int. 2022 Sep 24;169:107545. doi

    Insert

    mScarlet-I
    

    Use

    Tags

    mScarlet-I

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge