We narrowed to 10,452 results for: tet

1. pHAGE2-EF1a-4xGFP11-SEC61B-IRES-Hygro

   Plasmid

   #211468

   Purpose

   Lentivirus backbone expressing SEC61B protein tagged with 4 tandem GFP11 repeats at the N-terminal

   Depositor

   Agnel Sfeir

   Article

   Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

   Insert

   4xGFP11-SEC61B
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKA-207I10

   Plasmid

   #79845

   Purpose

   Expresses NLS-PpsR2-VP16-IRES-RpBphP1-mCherry-TetR under CMV promoter

   Depositor

   Vladislav Verkhusha

   Article

   Kaberniuk et al Nat Methods. 2016 May 9. doi: 10.1038/nm

   Insert

   NLS-PpsR2-VP16-IRESI10-BphP1-mCherry-TetR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   RpPpsR2 cysteine 439 changed to serine

   Promoter

   CMV

   Available since

   Aug. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLD1 translation factors

   Plasmid

   #117760

   Purpose

   BioBrick encoding cistrons 1-13 in pET for preparation of PURE 3.0 translation system from E. coli

   Depositor

   Anthony Forster

   Article

   Shepherd et al Nucleic Acids Res. 2017 Oct 13;45(18):10

   Insert

   Translation factor cistrons 1-13
   

   Use

   Escherichia coli

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTP813

   Plasmid

   #113308

   Purpose

   human Mre11 in baculovirus transfer vector

   Depositor

   Tanya Paull

   Article

   Bhaskara et al Mol Cell. 2007 Mar 9;25(5):647-61. doi:

   Insert

   Mre11 (MRE11 Human)
   

   Use

   Tags

   Flag

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin

   Available since

   Sept. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. SpCas9 nickase for Split-PE (pJUL2497)

   Plasmid

   #190104

   Purpose

   Expresses nickase SpCas9(H840A) for Split-PE use

   Depositor

   Keith Joung

   Article

   Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

   Insert

   nSpCas9
   

   Use

   Tags

   XTEN-bpNLS and bpNLS

   Expression

   Mammalian

   Mutation

   H840A

   Promoter

   CMV

   Available since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBS-TRE-tdKatushka2-WPRE

   Plasmid

   #193337

   Purpose

   Expresses tdKatushka2 under the TRE promoter in mammalian cells

   Depositor

   Takeshi Imai

   Article

   Leiwe et al Nat Commun. 2024 Jun 25;15(1):5279. doi:

   Insert

   tdKatushka2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE

   Available since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pVS2

   Plasmid

   #33144

   Depositor

   Michael Rosbash

   Article

   Vodala et al Mol Cell. 2008 Jul 11;31(1):104-13.

   Inserts

   LacO (256 copies)
   GAL-GFP-kd200-GALpA reporter
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   GFP is mutated such that amino acids 64-73 (FSYGV…

   Promoter

   bidirectional GAL1-GAL10 promoter

   Available since

   Dec. 20, 2011

   Availability

   Academic Institutions and Nonprofits only

8. pmTurquoise2-C1

   Plasmid

   #60560

   Purpose

   Expresses the cyan fluorescent protein variant mTurquoise2, can be used to create fusion proteins

   Depositor

   Dorus Gadella

   Article

   Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

   Insert

   mTurquoise2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSb_init containing anti SARS-CoV-2_RBD sybody Sb#45

   Plasmid

   #153526

   Purpose

   Expression of the anti SARS-CoV-2_RBD sybody Sb#45

   Depositor

   Markus Seeger

   Article

   Walter et al bioRxiv 2020.04.16.045419

   Insert

   Sb#45
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD

   Available since

   June 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pmTurquoise2-N1

    Plasmid

    #60561

    Purpose

    Expresses the cyan fluorescent protein variant mTurquoise2, can be used to create fusion proteins

    Depositor

    Dorus Gadella

    Article

    Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

    Insert

    mTurquoise2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. HERV-Kcon-RT for Split-PE (pJUL3120)

    Plasmid

    #190107

    Purpose

    Expresses HERV-Kcon RT (human codon optimization)

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

    Insert

    HERV-Kcon-RT
    

    Use

    Tags

    bpNLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. mTurquoise2-C1

    Plasmid

    #54842

    Purpose

    Localization: C1 Cloning Vector, Excitation: 434, Emission: 474

    Depositor

    Dorus Gadella, Michael Davidson

    Article

    Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

    Type

    Empty backbone

    Use

    Tags

    mTurquoise2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 12, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pSEVA221pmCDA1-T7RNAP

    Plasmid

    #167978

    Purpose

    Expresses fusion protein pmCDA1-T7RNAP in E. coli

    Depositor

    Luis Ángel Fernández

    Article

    Alvarez et al Nat Commun. 2020 Dec 22;11(1):6436. doi:

    Insert

    pmCDA1-T7RNAP (T7p07 Synthetic)
    

    Use

    Tags

    T7 tag and T7RNAP

    Expression

    Bacterial

    Mutation

    Promoter

    TetR-Ptet

    Available since

    June 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. PKAcs-GGSD5X-GFP

    Plasmid

    #108584

    Purpose

    Expresses PKAcs-GFP fusion in mammalian cells with flexible linker

    Depositor

    Robert Hughes

    Article

    Norris et al FEBS Open Bio. 2018 Apr 25;8(6):1029-104

    Insert

    protein kinase A, catalytic subunit
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    H87Q and W196R

    Promoter

    Available since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pmTurquoise2-NES

    Plasmid

    #36206

    Purpose

    In vivo visualization of the cytoplasm, but not the nucleus (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

    Insert

    NES
    

    Use

    Tags

    mTurquoise2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. Marathon-RT pentamutant for Split-PE (pEH107)

    Plasmid

    #190110

    Purpose

    Expresses Marathon-RT pentamutant (D14R-N26R-D74R-N116K-N197R)

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

    Insert

    Marathon-RT
    

    Use

    Tags

    bpNLS

    Expression

    Mammalian

    Mutation

    D14R, N26R, D74R, N116K, N197R

    Promoter

    CMV

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pNVLTv2-pvdIJD-Prha-base

    Plasmid

    #169244

    Purpose

    Suicide vector for integrating expression cassette in P. putida (L-rhamnose induction)

    Depositor

    Brian Pfleger

    Article

    Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

    Insert

    rhaRS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    rhaS

    Available since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. p12x601

    Plasmid

    #157785

    Purpose

    EcoRV digestion produces 12x601 chromatin assembly construct with central TetO following PEG removal of plasmid backbone

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    pMD2.G(spei/spei)&12x601_tetO
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pNAS1B

    Plasmid

    #61968

    Purpose

    Duet vector for co-expression of GFP and mCherry from PBAD and PLtetO promoters, respectively; parent vector for split mCherry assembly vectors

    Depositor

    Lynne Regan

    Article

    Sawyer et al ACS Chem Biol. 2014 Nov 21;9(11):2502-7.

    Inserts

    mCherry
    GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Serine 65 to Glycine (GGT), Serine 72 to Alanine …

    Promoter

    PBAD and PLtetO

    Available since

    Jan. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. TLCV2-ApaLI*-FLAG-T2A-mTagBFP2-Puro

    Plasmid

    #211463

    Purpose

    Lentivirus backbone expressing a doxycycline-inducible mitochondrial localized ApaLI mutant (D211A, E225A and K227A)

    Depositor

    Agnel Sfeir

    Article

    Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

    Insert

    ApaLI-dead
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    D211A, E225A, K227A

    Promoter

    TRE

    Available since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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