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pCMV-bpNLS-IS621 (CJT489)

Plasmid

#248213

Purpose

bpNLS-tagged IS621 bridge recombinase, expressed from a CMV promoter.

Depositor

Benjamin Kleinstiver

Article

Tou et al Nature. 2026 Mar 11. doi: 10.1038/s41586

Insert

bpNLS-IS621

Use

CRISPR; In vitro transcription

Tags

BPNLS

Expression

Mammalian

Mutation

None

Promoter

CMV and T7

Available Since

April 16, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV-SV40NLS-Stab-exin21-Bxb1(A315R)-Stab (CJT604)

Plasmid

#248210

Purpose

SV40 NLS-, stabilon-, and exin21-tagged Bxb1 (A315R) recombinase, expressed from a CMV promoter.

Depositor

Benjamin Kleinstiver

Article

Tou et al Nature. 2026 Mar 11. doi: 10.1038/s41586

Insert

SV40NLS-Stab-exin21-Bxb1(A315R)-Stab

Use

CRISPR; In vitro transcription

Tags

SV40NLS

Expression

Mammalian

Mutation

Bxb1(A315R)

Promoter

CMV and T7

Available Since

April 15, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV-bpNLS-Bxb1 (EMK569)

Plasmid

#248208

Purpose

Bxb1 recombinase with N-terminal bpNLS, expressed from a CMV promoter.

Depositor

Benjamin Kleinstiver

Article

Tou et al Nature. 2026 Mar 11. doi: 10.1038/s41586

Insert

bpNLS-Bxb1

Use

CRISPR; In vitro transcription

Tags

BPNLS

Expression

Mammalian

Mutation

None

Promoter

CMV and T7

Available Since

April 15, 2026

Availability

Academic Institutions and Nonprofits only

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pLKO-U6-Letm1shRNA-hPGK-Puro

Plasmid

#215362

Purpose

Expresses a rat Letm1 specific shRNA

Depositor

Jaime de Juan-Sanz

Article

Amrapali Vishwanath et al Nat Metab. 2026 Feb 11. doi: 10.1038/s42

Insert

Letm1 shRNA Target sequence (Letm1 Rat)

Use

Lentiviral

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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pGGG L2 TaStb15

Plasmid

#226630

Purpose

Wheat transformation vector used to express wheat Septoria tritici blotch (Stb) resistance gene 15

Depositor

Brande Wulff

Article

Hafeez et al Nat Plants. 2025 Mar;11(3):410-420. doi:

Insert

Triticum aestivum (wheat) Septoria tritici blotch 15 (TaStb15) disease resistance gene

Use

Synthetic Biology

Expression

Plant

Promoter

Native TaStb15

Available Since

Aug. 15, 2025

Availability

Academic Institutions and Nonprofits only

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AURKA-Aquamarine

Plasmid

#228564

Purpose

Encodes a donor-only, Aquamarine-tagged version of the AURKA biosensor under the control of a CMV promoter

Depositor

Giulia Bertolin

Article

Bousmah et al ACS Sens. 2021 Nov 26;6(11):3940-3947. d

Insert

AURKA (AURKA, AURKA Human)

Tags

Aquamarine

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pWT-IFl_WT-IFr

Plasmid

#192207

Purpose

Wildtype I-F CRISPR Leader and a CRISPR locus with two wildtype I-F repeats, for integration assays.

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

Wildtype I-F Leader and wildtype I-F Locus DNA

Expression

Bacterial

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pScr-IFl_WT-IFr

Plasmid

#192208

Purpose

Scrambled I-F CRISPR Leader and a CRISPR locus with two wildtype I-F repeats, for integration assays.

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

Scrambled I-F Leader and wildtype I-F Locus DNA

Expression

Bacterial

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCon-IFl_Con-IFr

Plasmid

#192209

Purpose

Consensus I-F CRISPR Leader and a CRISPR locus with two consensus I-F repeats, for integration assays.

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

Consensus I-F Leader and Consensus I-F Locus DNA

Expression

Bacterial

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCon-IFl_Con-IEr

Plasmid

#192210

Purpose

Consensus I-F CRISPR Leader and a CRISPR locus with two consensus I-E repeats, for integration assays.

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

Consensus I-F Leader and Consensus I-E Locus DNA

Expression

Bacterial

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCon-IFl_Con-ICr

Plasmid

#192211

Purpose

Consensus I-F CRISPR Leader and a CRISPR locus with two consensus I-C repeats, for integration assays.

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

Consensus I-F Leader and Consensus I-C Locus DNA

Expression

Bacterial

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCon-IFl_Con-IIAr

Plasmid

#192212

Purpose

Consensus I-F CRISPR Leader and a CRISPR locus with two consensus II-A repeats, for integration assays.

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

Consensus I-F Leader and Consensus II-A Locus DNA

Expression

Bacterial

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCon-IEl_Con-IEr

Plasmid

#192213

Purpose

Consensus I-E CRISPR Leader and a CRISPR locus with two consensus I-E repeats, for integration assays.

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

Consensus I-E Leader and Consensus I-E Locus DNA

Expression

Bacterial

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCon-ICl_Con-ICr

Plasmid

#192214

Purpose

Consensus I-C CRISPR Leader and a CRISPR locus with two consensus I-C repeats, for integration assays.

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

Consensus I-C Leader and Consensus I-C Locus DNA

Expression

Bacterial

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCon-IIAl_Con-IIAr

Plasmid

#192215

Purpose

Consensus II-A CRISPR Leader and a CRISPR locus with two consensus II-A repeats, for integration assays.

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

Consensus II-A Leader and Consensus II-A Locus DNA

Expression

Bacterial

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pX1-IFl_Con-IFr

Plasmid

#192216

Purpose

Mutant I-F CRISPR Leader and a CRISPR locus with two consensus I-F repeats, for integration assays.

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

I-F leader and I-F repeat construct not studied further in this paper (X1)

Expression

Bacterial

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pX2-IFl_Con-IFr

Plasmid

#192217

Purpose

Mutant I-F CRISPR Leader and a CRISPR locus with two consensus I-F repeats, for integration assays.

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

I-F leader and I-F repeat construct not studied further in this paper (X2)

Expression

Bacterial

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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VEGF120TAG

Plasmid

#200946

Purpose

Encodes recombinant mouse VEGF120 when coupled with amber stop codon suppression by non-canonical amino acid incorporation

Depositor

Elizabeth Kirby

Article

Miller et al Mol Psychiatry. 2024 Nov 11. doi: 10.103

Inserts

VEGF120TAG (Vegfa Mouse)
hPGK-driven Puromycin resistance

Use

Lentiviral

Tags

6x HIS

Mutation

amber stop codon 101 bp from ATG

Promoter

Ef1a and hPGK

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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VEGF164TAG

Plasmid

#200947

Purpose

Encodes recombinant mouse VEGF164 when coupled with amber stop codon suppression by non-canonical amino acid incorporation

Depositor

Elizabeth Kirby

Article

Miller et al Mol Psychiatry. 2024 Nov 11. doi: 10.103

Inserts

VEGF164TAG (Vegfa Mouse)
hPGK-driven Puromycin resistance

Use

Lentiviral

Tags

6x HIS

Mutation

amber stop codon 101 bp from ATG

Promoter

Ef1a and hPGK

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-GFP-LA

Plasmid

#206197

Purpose

Expresses GFP-tagged human Lamin-A protein by the constitutive CMV promoter in mice

Depositor

Yuxuan Guo

Article

Sun et al JACC Basic Transl Sci. 2024 Aug 21;9(11)

Insert

Lmna (LMNA )

Use

AAV

Tags

GFP

Promoter

CMV

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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