We narrowed to 10,140 results for: CCH

1. HsSPHK2

   Plasmid

   #118092

   Purpose

   Expresses sphingosine kinase 2, which catalyzes the phosphorylation of sphingosine to sphingosine-1-phosphate (S1P)

   Depositor

   Kevin Lynch

   Article

   Kharel et al PLoS One. 2018 Apr 19;13(4):e0192179. do

   Insert

   Homo sapiens Sphingosine Kinase 2 (SPHK2 Human)
   

   Tags

   FLAG

   Expression

   Yeast

   Promoter

   GAL1, 10

   Available Since

   Aug. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET151-Vps4(101-437)

   Plasmid

   #87735

   Purpose

   Bacterial expression of N-terminally truncated S. cerevisiae Vps4

   Depositor

   Chris Hill

   Article

   Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

   Insert

   Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
   

   Tags

   6xHis-V5-TEV

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-100

   Promoter

   T7

   Available Since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pG4-E6s-luc

   Plasmid

   #46324

   Depositor

   Steven Brown

   Article

   Kowalska et al Mol Cell Biol. 2012 Nov;32(22):4585-94.

   Insert

   5xGal4
   

   Use

   Luciferase

   Tags

   E boxes and luciferase

   Expression

   Mammalian

   Mutation

   five copies of a multimerized GAL4 (5×GAL4) site …

   Available Since

   July 5, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pHCas9-Nours

   Plasmid

   #107733

   Purpose

   Escherichia coli- S. cerevisiae shuttle plasmid harbors a Cas9 gene, a natMX6 and URA3 selection markers used in S. cerevisiae

   Depositor

   Sheng Yang

   Article

   Wang et al Biotechnol Bioeng. 2018 Sep 10. doi: 10.

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Yeast

   Mutation

   Human optimized

   Promoter

   TEF1

   Available Since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCI-FLAG-mTUT4(D1028A)

   Plasmid

   #60220

   Purpose

   Mammalian expression of FLAG-tagged catalytic mutant of TUT4

   Depositor

   Zissimos Mourelatos

   Article

   Liu et al Mol Cell. 2014 Aug 27. pii: S1097-2765(1

   Insert

   TUT4 (Tut4 Mouse)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   D1028A

   Promoter

   CMV

   Available Since

   Dec. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pUAST mito roGFP2-Orp1

   Plasmid

   #64997

   Purpose

   for expression of mitochondrial roGFP2-Orp1 with the Gal4 expression system in drosophila

   Depositor

   Tobias Dick

   Article

   Albrecht et al Cell Metab. 2011 Dec 7;14(6):819-29. doi

   Insert

   Orp1 (HYR1 Budding Yeast)
   

   Tags

   roGFP2

   Expression

   Insect

   Mutation

   mitochondrial targeting sequence

   Available Since

   June 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGST-TEV-yVms1(1-417,delta(38-69))

   Plasmid

   #128739

   Purpose

   Bacterial overexpression and crystallization

   Depositor

   Chris Hill

   Article

   Nielson et al Mol Cell. 2017 Nov 16;68(4):673-685.e6.

   Insert

   Vms1 (VCP/Cdc48-associated mitochondria stress-responsive 1) (VMS1 Budding Yeast)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   residues 1-417 with deletion of 38-69

   Promoter

   T7

   Available Since

   Nov. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET-HJURP-CBP

   Plasmid

   #30393

   Depositor

   Yawen Bai

   Article

   Zhou et al Nature. 2011 Apr 14. 472(7342):234-7.

   Insert

   HJURP CenpA Binding Domain (CENPA Human, Budding Yeast)
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   July 11, 2011

   Availability

   Academic Institutions and Nonprofits only

9. NAX_CAGGS_CAS9_m4_VPR_EYFP

   Plasmid

   #167882

   Purpose

   PiggyBac compatible plasmid expressing dCas9-VPR

   Depositor

   Alejandro Chavez, Nan Cher Yeo

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   dCas9-VPR
   

   Use

   CRISPR

   Mutation

   D10A, D839A, H840A, N863A

   Available Since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pITGFP89

    Plasmid

    #83560

    Purpose

    Used to evaluate the expression output of TEF1 promoter with yEGFP-CLN2PEST (destabilized GFP) used as the reporter gene.

    Depositor

    Claudia Vickers

    Article

    Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

    Insert

    TEF1 promoter -yEGFP-CLN2PEST
    

    Expression

    Yeast

    Promoter

    TEF1

    Available Since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCfB2989

    Plasmid

    #63636

    Purpose

    pCfB2989 is a vector for multiple integrations at sites sharing homology with Ty1Cons1. The selective marker is Kl.URA3. Additionally it contains a USER cassette.

    Depositor

    Irina Borodina, Jerome Maury

    Article

    Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

    Type

    Empty backbone

    Use

    Cre/Lox

    Expression

    Yeast

    Promoter

    none

    Available Since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pFS485

    Plasmid

    #89348

    Purpose

    Leu1 integrating plasmid with cdc25 genomic fragment

    Depositor

    Nick Rhind

    Article

    Keifenheim et al Curr Biol. 2017 May 22;27(10):1491-1497.

    Insert

    cdc25 (cdc25 Fission Yeast)
    

    Expression

    Yeast

    Available Since

    July 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. IpO

    Plasmid

    #48233

    Purpose

    Base vector for PCR-based DNA transplant in yeast. DNA segments to be transplanted into the yeast genome are cloned into the MCS.

    Depositor

    Ronald Davis

    Article

    Horecka et al Yeast. 2014 May;31(5):185-93. doi: 10.10

    Inserts

    URA3 3' fragment
    URA3 5' fragment
    

    Use

    Routine cloning vector

    Mutation

    -242 upstream to URA3 ORF +495 and URA3 ORF from …

    Promoter

    URA3 promoter and none

    Available Since

    Feb. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pNB813

    Plasmid

    #60161

    Purpose

    Yeast codon-optimized yellow fluorescent protein (yEVenus) with PEST degron driven by SIC1 promoter.

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    yEVenus-PEST
    

    Tags

    PEST degron

    Expression

    Yeast

    Promoter

    SIC1pr

    Available Since

    March 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFA6a-kanMX6-Purg1-GFP

    Plasmid

    #39353

    Depositor

    Jurg Bahler

    Article

    Watt et al PLoS One. 2008 Jan 16;3(1):e1428.

    Insert

    urg1 promoter (urg1, urg1 Fission Yeast)
    

    Use

    Yeast genomic targeting

    Tags

    GFP and kanMX6 module - reverse orientation

    Available Since

    Nov. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pXylA-RgTALsyn

    Plasmid

    #100948

    Purpose

    Expression of Tyrosine Ammonia Lyase from constitutive XylA promoter

    Depositor

    Mattheos Koffas

    Article

    Jones et al MBio. 2017 Jun 6;8(3). pii: e00621-17. d

    Insert

    RgTALsyn
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    Silent mutation to remove internal NdeI site on T…

    Promoter

    XylA (Constitutive)

    Available Since

    Sept. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEGFP_C1 I3

    Plasmid

    #61397

    Purpose

    Expresses GFP-tagged Inhibitor-3 in mammalian cells

    Depositor

    Hemmo Meyer

    Article

    Eiteneuer et al EMBO J. 2014 Oct 8. pii: e201489054.

    Insert

    Inhibitor-3 (PPP1R11 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pGBDU-Atg17

    Plasmid

    #121394

    Purpose

    For Yeast-two-hybrid analysis

    Depositor

    Daniel Klionsky

    Article

    Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

    Insert

    ATG17
    

    Expression

    Yeast

    Promoter

    ADH1 promoter

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. POT10-RFP

    Plasmid

    #65326

    Purpose

    receiving vector of standard transcription units(TUs) in YeastFab work, useful for further assembly

    Depositor

    Junbiao Dai

    Article

    Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

    Insert

    RFP
    

    Use

    Synthetic Biology

    Available Since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. POT8-RFP

    Plasmid

    #65324

    Purpose

    receiving vector of standard transcription units(TUs) in YeastFab work, useful for further assembly

    Depositor

    Junbiao Dai

    Article

    Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

    Insert

    RFP
    

    Use

    Synthetic Biology

    Available Since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge