We narrowed to 3,382 results for: CAMP

1. pLenti-CMV-Pink Flamindo-p2a-bPAC

   Plasmid

   #202716

   Purpose

   Mammalian co-expression of soluble Pink Flamindo, a fluorescent cAMP indicator and soluble bPAC, a photoactivatable adenylyl cyclase

   Depositor

   Adam Cohen

   Article

   Xiang et al bioRxiv 2023

   Inserts

   Pink Flamindo
   bPAC
   

   Use

   Lentiviral

   Tags

   10xHis and myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pUC57-gCarvi-T2A

   Plasmid

   #190333

   Purpose

   Fluorescent reporter for cAMP

   Depositor

   Naoto Saitoh

   Article

   Kawata et al Proc Natl Acad Sci U S A. 2022 Jul 12;11

   Insert

   gCarvi
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pUC57-rm_gCarvi-T2A

   Plasmid

   #190334

   Purpose

   Ratiometric reporter for cAMP

   Depositor

   Naoto Saitoh

   Article

   Kawata et al Proc Natl Acad Sci U S A. 2022 Jul 12;11

   Insert

   ratiometric gCarvi
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. nls-Flamindo2

   Plasmid

   #73939

   Purpose

   Expresses biosensor for cAMP in mammalian cells with nuclear localization signal

   Depositor

   Tetsuya Kitaguchi

   Article

   Odaka et al PLoS One. 2014 Jun 24;9(6):e100252. doi:

   Insert

   nls-Flamindo2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCalpha EV (PKA catalytic subunit Calpha)

   Plasmid

   #15310

   Depositor

   Stanley McKnight

   Article

   Uhler et al J Biol Chem. 1987 Nov 5. 262(31):15202-7

   Insert

   PRKACA (Prkaca Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 6, 2007

   Availability

   Academic Institutions and Nonprofits only

6. pCbeta EV (PKA catalytic subunit Cbeta)

   Plasmid

   #15311

   Depositor

   Stanley McKnight

   Article

   Uhler et al J Biol Chem. 1987 Nov 5. 262(31):15202-7

   Insert

   PRKACB (Prkacb Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 6, 2007

   Availability

   Academic Institutions and Nonprofits only

7. pLenti-CMV-Pink Flamindo-p2a-paGFP-bPAC-CAAX

   Plasmid

   #202718

   Purpose

   Mammalian co-expression of soluble Pink Flamindo and membrane-bound bPAC for cAMP perturbation and imaging

   Depositor

   Adam Cohen

   Article

   Xiang et al bioRxiv 2023

   Inserts

   Pink Flamindo
   paGFP-bPAC-CAAX
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLenti-CMV-Myr-Pink Flamindo-p2a-EGFP-bPAC

   Plasmid

   #202717

   Purpose

   Mammalian co-expression of membrane-bound Pink Flamindo and soluble bPAC for cAMP perturbation and imaging

   Depositor

   Adam Cohen

   Article

   Xiang et al bioRxiv 2023

   Inserts

   Myr-Pink Flamindo
   EGFP-bPAC
   

   Use

   Lentiviral

   Tags

   10xHis and myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pWT PolI

   Plasmid

   #11721

   Depositor

   Lawrence Loeb

   Article

   Camps et al Proc Natl Acad Sci U S A. 2003 Aug 19. 1

   Insert

   DNA polymerase I (polA E. coli)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   wild-type

   Promoter

   Available since

   Aug. 8, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Pink Flamindo

    Plasmid

    #102356

    Purpose

    biosensor for cAMP in mammalian cells

    Depositor

    Tetsuya Kitaguchi

    Article

    Harada et al Sci Rep. 2017 Aug 4;7(1):7351. doi: 10.1

    Insert

    Pink Flamindo
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLenti-CMV-Myr-Pink Flamindo-p2a-EGFP-bPAC-CAAX

    Plasmid

    #202719

    Purpose

    Mammalian co-expression of membrane-bound Pink Flamindo and membrane-bound bPAC for cAMP perturbation and imaging

    Depositor

    Adam Cohen

    Article

    Xiang et al bioRxiv 2023

    Inserts

    Myr-Pink Flamindo
    EGFP-bPAC-CAAX
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEYFP-N3-EPAC1

    Plasmid

    #113110

    Purpose

    Human EPAC1 gene was fused in-frame and upstream from the enhanced YFP gene in pEYFP-N3 vector (Clonetech).

    Depositor

    Xiaodong Cheng

    Article

    Mei et al J Biol Chem. 2002 Mar 29;277(13):11497-5

    Insert

    RapGEF3 (RAPGEF3 Human)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    PCMV

    Available since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Epac-S-H94

    Plasmid

    #170341

    Purpose

    GFP(UST)_EPACdDEPCD_mCherry biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

    Depositor

    Kees Jalink

    Article

    Klarenbeek et al PLoS One. 2015 Apr 14;10(4):e0122513. do

    Insert

    GFP(UST)_EPACdDEPCD_mCherry (RAPGEF3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Deletion of DEP domain, Catalytically dead T781A …

    Promoter

    CMV

    Available since

    June 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEP PolI

    Plasmid

    #11722

    Depositor

    Lawrence Loeb

    Article

    Camps et al Proc Natl Acad Sci U S A. 2003 Aug 19. 1

    Insert

    DNA polymerase I EP (polA E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D424A, I709N, A759R. Error-prone version of PolI.

    Promoter

    Available since

    Aug. 8, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1(+)-FluoSTEP-ICUE

    Plasmid

    #181845

    Purpose

    Genetically encoded FRET-based sensor for monitoring cAMP dynamics near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

    Depositor

    Jin Zhang

    Article

    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

    Insert

    FluoSTEP-ICUE (RAPGEF3 Human)
    

    Use

    Tags

    mRuby2 and sfGFP(1-10)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3-PDE4D2cat-ICUE4

    Plasmid

    #181847

    Purpose

    ICUE4 cAMP sensor tethered to the catalytic domain of PDE4D2.

    Depositor

    Jin Zhang

    Article

    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

    Insert

    PDE4D2cat-ICUE4 (RAPGEF3, PDE4D Human)
    

    Use

    Tags

    ECFP, PDE4D2(86-418), and cpVenus(L194)

    Expression

    Mammalian

    Mutation

    Glutamine 122 in Epac1 domain mutated to glutamat…

    Promoter

    CMV

    Available since

    June 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1(+)-R/G-ICUE

    Plasmid

    #181849

    Purpose

    ICUE cAMP sensor using sfGFP and mRuby2 as the FRET donor and acceptor.

    Depositor

    Jin Zhang

    Article

    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

    Insert

    R/G-ICUE (RAPGEF3 Human)
    

    Use

    Tags

    mRuby2 and sfGFP

    Expression

    Mammalian

    Mutation

    Glutamine 122 in Epac1 domain mutated to glutamat…

    Promoter

    CMV

    Available since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. AAV-CREB

    Plasmid

    #68550

    Purpose

    AAV expression of CREB

    Depositor

    Eric Nestler

    Article

    Berger et al Biol Psychiatry. 2011 Feb 1;69(3):288-94

    Insert

    CREB
    

    Use

    AAV

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AAV-mCREB

    Plasmid

    #68551

    Purpose

    AAV expression of mCREB

    Depositor

    Eric Nestler

    Article

    Berger et al Biol Psychiatry. 2011 Feb 1;69(3):288-94

    Insert

    mCREB
    

    Use

    AAV

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S133A

    Promoter

    CMV

    Available since

    Sept. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA3-RIα-ICUE4

    Plasmid

    #181848

    Purpose

    ICUE4 cAMP sensor tethered to the PKA RIα subunit.

    Depositor

    Jin Zhang

    Article

    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

    Insert

    RIα-ICUE4 (RAPGEF3, PRKAR1A Human)
    

    Use

    Tags

    ECFP, PKA RIα, and cpVenus(L194)

    Expression

    Mammalian

    Mutation

    Glutamine 122 in Epac1 domain mutated to glutamat…

    Promoter

    CMV

    Available since

    June 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge