We narrowed to 109 results for: CAMP

...pRSET OCaMP pRSET T7 pAAV-hSYN1-NES-OCaMP-WPRE pAAV hSyn pAAV-EF1a-FLEX(FLP)-OCaMP-WPRE...entire visible spectrum (Figure 1). The GCaMP series (e.g., jGCaMP8) remains the gold standard for green ... International license. Using OCaMP in your lab Several OCaMP constructs are now available through...Ef1a pAAV-hSyn-FLEX(cre)-OCaMP-WPRE pAAV hSyn pAAV-CMV-OCaMP-WPRE pAAV CMV The Lohman...team recently developed a new calcium indicator, OCaMP. The challenge: Expanding the fluorescent toolbox...fluorophores. The spectra for mOrange2 (scaffold of OCaMP) are shaded in orange. Data from FPbase.org. ...fluorescence, while red indicators jRGECO1a and jRCaMP1a are widely used as well. To complement these tools...

...transcription to begin. In addition to arabinose, cAMP complexed with cAMP activator protein (CAP) can also stimulate...Supplementing cell growth media with glucose decreases cAMP and represses pBad, decreasing promoter leakiness...

... pii: 11/520/eaah3738. Justin Blau cAMP (cyclic AMP) cAMPFIRE cAMP biosensors with improved sensitivity...Commun. 2013;4:2550. Gary Yellen cAMP (cyclic AMP) Yellow fluorescent cAMP indicator (Flamindo2) Genetically-encoded...e100252. Tetsuya Kitaguchi cAMP (cyclic AMP) Red fluorescent protein-based cAMP indicator (Pink Flamindo...subcellular imaging of cAMP in vivo. Nat Methods. 2022 Nov;19(11):1461-1471. Haining Zhong cAMP (cyclic AMP) RAB-ICUE...-0200-6. Jin Zhang cAMP (cyclic AMP) Yellow-emitting homoFRET/anisotropy ICUE cAMP sensor Single-color...-8674(20)31399-4. Edward Boyden cAMP (cyclic AMP) FRET sensors of cAMP levels for imaging in live cells...e0122513. Kees Jalink cAMP (cyclic AMP) Fluorescent protein exchange sensor for cAMP Ratiometric biosensors...

...in 2010 for visualizing the transcription factor cAMP-responsive element-binding protein (CREB) in living...were then refined by a secondary screen in rat hippocampal neuron cultures. The optimized Twitch sensors...

...previous publications. They also developed calcium, cAMP, and ATP sensors based on Nano-lantern, showing ...

...cytoskeleton components) and in a FRET-based biosensor for cAMP dynamics. The mGold2 variants significantly enhanced...

...pathways HDR mechanisms can be divided into two main camps: non-conservative and conservative. The conservative...

.... "Fourth-generation epac-based FRET sensors for cAMP feature exceptional brightness, photostability and...

...Decrease cAMP Neuronal inhibition Armbruster et al., 2007 KORD Human κ-opioid G αi SALB Decrease cAMP Neuronal...signaling by inhibiting adenylate cyclase and downstream cAMP production. There are currently two Gi DREADDs based...activate neuronal signaling by increasing intracellular cAMP concentrations. There is currently one Gs-DREADD...muscarinic & turkey β1-adrenergic G αs CNO* Increase cAMP Neuronal burst firing Guettier et al., 2009 hM4Di.... A Gαs DREADD Mouse for Selective Modulation of cAMP Production in Striatopallidal Neurons. Neuropsychopharmacol...

...adenylyl cyclase from Beggiatoa sp. for light-induced cAMP modulation. Utilizes BLUF (blue light utilizing ...

...There are three GCaMP types based on their kinetics: GCaMPf (fast) GCaMPm (medium), GCaMPs (sensitive but...zebrafish and mammalian systems. The GCaMP6, jGCaMP7, and jGCaMP8 sensors are also suitable for in vivo...slower). GCaMPf may be suitable for very rapid measurements, like action potentials in neurons. jGCaMP7 and...localize at the plasma membrane. GCaMP-X is a nucleus-targeted GCaMP6 with an extra apoCaM-binding motif...axon (GCaMP6 only). The genetic elements required for Drosophila studies have been added for jGCaMP7 and...to have slower, but larger, calcium changes. The GCaMP6 family of sensors are commonly used calcium reporters...and jGCaMP8 are also available with improved kinetic and sensitivity parameters. Sensitivity and Localization...

...requests faster than jGCaMP7 (Figure 5B).   Figure 5: A) GCaMP6, jGCaMP7, and jGCaMP8 viral preps distributed...Figure 5A). We then added jGCaMP7 and eventually jGCaMP8, later generations of GCaMP6, to the service. While...) Inset showing distribution of only GCaMP6, jGCaMP7, and jGCaMP8 per year from 2016–2023. All three...generations: jGCaMP8 allows for the “picture” of a train of action potentials that GCaMP6 and jGCaMP7 do not... for instance, the GCaMP-encoding viral vectors available in the repository. GCaMP, a calcium indicator...they were both requested, GCaMP6 remains the more requested option, and jGCaMP8 appears to be growing in...be a matter of timing, as jGCaMP7 was released relatively soon after GCaMP6. It could be a matter of the...

...proteins, PinkyCaMP is the first based on mScarlet. Compared to RCaMP3 and jRGECO1a, PinkyCaMP had better...improvements. Find PinkyCaMP plasmids and FRCaMPi plasmids! Fink, R., Imai, S., et al. (2024). PinkyCaMP a mScarlet-based...transfer tracker Red calcium indicators PinkyCaMP and FRCaMPi Micropeptide killswitch for biomolecular....0510511103      Red calcium indicators PinkyCaMP and FRCaMPi By Mike Lacy Many popular genetically encoded...the spectrum: PinkyCaMP from the Olivia Masseck Lab (Fink & Imai et al., 2024) and FRCaMPi from the Kiryl... could multiplex PinkyCaMP alongside green fluorescent and optogenetic tools. FRCaMPi uses an inverted...Although similar in brightness to jRGECO1a and FRCaMP, FRCaMPi had better dynamic range and sensitivity, in...

...-fold brighter than GCaMP6s. jGCaMP7f is the fastest responding variant. jGCaMP7b exhibits the brightest...pGP-AAV-syn-jGCaMP7f-WPRE (104488-AAV8) pGP-AAV-syn-FLEX-jGCaMP7f-WPRE (104492-AAV8) pGP-AAV-syn-jGCaMP7c variant...potential, GCaMP7s is approximately twice as bright, relative to baseline fluorescence, as the GCaMP7f variant...potentials. This is about twice as bright as GCaMP7f and GCaMP7s. See all our biosensors inventory, and check...Calcium Sensors pGP-AAV-syn-jGCaMP7b-WPRE (104489-AAVrg) pGP-AAV-syn-FLEX-jGCaMP7b-WPRE (104493-AAVrg) pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE...pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE (104495-AAVrg) pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE (104496-AAVrg) Optogenetics...sensors The latest calcium sensors from Janelia (jGCaMP7) continue to be useful for monitoring neuronal ...

...Flp-dependent GCaMP, AAV8-105714. Several new options are also available for the calcium sensor jGCaMP7 from Janelia... Janelia, including three jGCaMP7 plasmids now packaged in AAVrg. jGCaMP7s, the variant with the most ...104489 AAVrg pGP-AAV-syn-jGCaMP7b-WPRE 104493 AAVrg pGP-AAV-syn-FLEX-jGCaMP7b-WPRE 104496 AAVrg pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE...pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE 104495 AAVrg GP-AAV-CAG-FLEX-jGCaMP7s-WPRE 105714 AAV8 pAAV-Ef1a-fDIO-GCaMP6s...pAAV-Ef1a-fDIO-GCaMP6s Neurotransmitter sensors: Dopamine and norepinephrine Neuroscience research relies on accurate...

...pGP-AAV-syn-FLEX-jGCaMP7b-WPRE) exhibits the brightest resting fluorescence of the GCaMP7 family of calcium...inventory! Our new AAVs include: pGP-AAV-syn-FLEX-jGCaMP7b-WPRE pAAV.hSyn-FLEX.iGABASnFR pAAV.hSyn-FLEX.iGABASnFR.F102G...calcium binding. There are a few versions of these jGCaMP7 sensors with different properties (for example,...applications. We recently released a new variant of jGCaMP7 (keep reading) and will be releasing more variants...like dendrites and axons. Find the 7b variant of jGCaMP7 in AAV1 at Addgene! See all calcium sensor AAV ...Serotype Name 104496  AAV1  GP-AAV-CAG-FLEX-jGCaMP7f-WPRE AAV1 with a CAG promoter exhibits high specificity...Purkinje neurons. 105321  AAV1  pGP-AAV-syn-jGCaMP7c variant 1513-WPRE 7c exhibits high contrast between...

...axon-GCaMP6s-P2A-mKate2 (AAV9) pAAV-hSynapsin1-GCaMP6s-P2A-mKate2 (AAV9) pAAV-hSynapsin1-GCaMP6s-P2A-mRuby3...AAV9) pAAV-CAG-SomaGCaMP6f2 (AAV9) Plus, additional AAV serotypes of the most recent jGCaMP8 calcium sensors...sensors: pAAV-hSynapsin1-axon-jGCaMP8s-P2A-mRuby3 (AAV1) pGP-AAV-syn-jGCaMP8f-WPRE (AAV Retrograde) pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE...-AAV-CAG-FLEX-jGCaMP8m-WPRE (AAV Retrograde) pGP-AAV-syn-FLEX-jGCaMP8m-WPRE (AAV5)   There are hundreds...dLight1.2 and voltage sensor Ace2N and additional GCaMP6 constructs: pAAV-CAG-dLight1.2 (AAV1) pGP-AAV-...

...  AAV1 pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE 105322  AAV1 pGP-AAV-syn-FLEX-jGCaMP7c variant 1513-WPRE 111262...AAV1 pAAV-hSynapsin1-axon-GCaMP6s 112005  AAV9 pAAV-hSynapsin1-axon-GCaMP6s-P2A-mRuby3) 112010  AAV9... pGP-AAV-syn-FLEX-jGCaMP7c variant 1513-WPRE 105714  AAV8  pAAV-Ef1a-fDIO-GCaMP6s Optogenetics ...AAVs include: Control AAVs for serotype testing jGCaMP7c for calcium detection PSAM4 GlyR for chemogenetic...Among other new biosensor tools, we now offer the jGCaMP7c variant in AAV1. This variant exhibits high contrast...AAV9 pAAV-hSynapsin1-FLEx-axon-GCaMP6s New voltage sensors Plasmid Serotype Name 119036  AAV1  ...

...repository, you'll find the latest iteration of GCaMP, GCaMP8, available in new serotypes, as well as seven...Notes AAV-CAG-jGCaMP8s-WPRE AAV1 Biosensor Looger New serotype AAV-CamKIIa-jGCaMP8m-WPRE AAV5 Biosensor...pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE AAV PHP.eB Biosensor Kim New serotype AAV-mDlx-jGCaMP8s-WPRE  AAV9 Biosensor...serotype AAV-hSyn-Soma-jGCaMP8s AAV9 Biosensor Fyhn New serotype AAV-hSyn-Soma-jGCaMP8f AAV9 Biosensor Fyhn...

...logistical stress that accompanies traveling for campus visits. This is definitely an advantage of surviving...everything you need to during the interview. (Virtual) Campus Visits After receiving applications, universities...students to continue the application process through a campus visit, a weekend where applications can get to ...allow you to get important information about the campus even if you can’t visit. By talking with people...aspects that are confusing? During a traditional campus visit, many students will have questions about ... be your perfect fit! Even if you had planned a campus visit and the in-person portion got canceled, taking...And even if you intend to attend an interview or campus visit in person, things like sickness or scheduling...