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Bxb1-GT_TRE-mScarlet

Plasmid

#229796

Purpose

Bxb1-GT donor plasmid for doxycycline-inducible mScarlet expression

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

mScarlet

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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11_T7-SP-mScarlet3-GPI

Plasmid

#229806

Purpose

In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with a signal peptide and a GPI attachment site

Depositor

Michalis Averof

Article

Karapidaki et al bioRxiv 2024.11.12.623055

Insert

SP-mScarlet3-GPI

Use

In vitro transcription

Mutation

T7 promoter

Available Since

Feb. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR1A 2xHA HCAR3

Plasmid

#226807

Purpose

Gateway entry vector for 2xHA HCAR3

Depositor

Ramiro Iglesias-Bartolome

Article

Pedro et al J Invest Dermatol. 2024 Jun;144(6):1311-

Insert

HCAR3 (HCAR3 Human)

Use

Gateway entry

Tags

2xHA tag followed by a GDPPVAT linker

Mutation

Sequence optimized from NP_006009.2

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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6_T7-Lyn-mScarlet3

Plasmid

#225933

Purpose

In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the Lyn membrane localisation signal

Depositor

Michalis Averof

Article

Karapidaki et al bioRxiv 2024.11.12.623055

Insert

Lyn-mScarlet3

Use

In vitro transcription

Promoter

T7

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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7_T7-Src64B-mScarlet3

Plasmid

#225934

Purpose

In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the Src64B membrane localisation signal

Depositor

Michalis Averof

Article

Karapidaki et al bioRxiv 2024.11.12.623055

Insert

Src64B-mScarlet3

Use

In vitro transcription

Promoter

T7

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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9_T7-mScarlet3-LactC2

Plasmid

#225936

Purpose

In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the LactC2 membrane localisation signal

Depositor

Michalis Averof

Article

Karapidaki et al bioRxiv 2024.11.12.623055

Insert

mScarlet3-LactC2

Use

In vitro transcription

Promoter

T7

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2-hcar1-4-mKate2

Plasmid

#226712

Purpose

overexpression red fluorescent hcar1-4 protein in zebrafish larvae

Depositor

Philipp Niethammer

Article

Ma et al Cell Rep. 2023 Jan 31;42(1):111974. doi:

Insert

hcar1-4

Use

Unspecified; Zebrafish expression

Tags

mKate2

Promoter

SP6

Available Since

Nov. 8, 2024

Availability

Academic Institutions and Nonprofits only

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CUP1-∆ssCPY*-mScarletI

Plasmid

#221157

Purpose

Fluorescent reporter for cytoplasmic aggregates

Depositor

Mark Leake

Insert

Carboxypeptidase Y (PRC1 Budding Yeast)

Tags

mScarletI

Expression

Yeast

Mutation

Deletion signal peptide, G255A mutation

Available Since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pET28-a(+)-6xHis-mScarlet-I(+5)

Plasmid

#219418

Purpose

Fluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysate

Depositor

Allie Obermeyer

Article

Ahn et al JACS Au. 2024 Sep 17;4(10):3800-3812. do

Insert

mScarlet-I(+5)

Tags

MGHHHHHGG

Expression

Bacterial

Mutation

D125K, D186K, E216K, E238K

Promoter

T7

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pIG-858_CD19-BBz_CAR_TFAP4

Plasmid

#207503

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

TFAP4, CD19-BBz_CAR (TFAP4 Human)

Use

CRISPR

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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mito-CCM2-mScarlet-I

Plasmid

#208886

Purpose

Express mScarlet-I-CCM2 targeted to the mitochondria in mammalian cells.

Depositor

Guillaume Jacquemet

Article

Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

Insert

CCM2 (CCM2 Human)

Tags

mScarlet-I

Expression

Mammalian

Mutation

CCM2 targeted to the mitonchondria

Available Since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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mito-PDCD10-mScarlet-I

Plasmid

#208887

Purpose

Express mScarlet-I-PDCD10 targeted to the mitochondria in mammalian cells.

Depositor

Guillaume Jacquemet

Article

Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

Insert

CCM3 (PDCD10 Human)

Tags

mScarlet-I

Expression

Mammalian

Mutation

PDCD10 targeted to the mitonchondria

Available Since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV_CKII(0.4)_AzPPO_D14_ts_mScarlet_Kv2.1

Plasmid

#205288

Purpose

Bistable opsin for neuronal inhibition

Depositor

Matthias Prigge

Insert

Parapinopsin

Use

AAV

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD-HisD-mCardinal

Plasmid

#197258

Purpose

Expresses the protein mCardinal in bacteria

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

mCardinal

Expression

Bacterial

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW ymTq2-ymScarletI

Plasmid

#205512

Purpose

Expression of ymTq2-ymScarletl in yeast

Depositor

Bas Teusink

Article

Botman et al bioRxiv 2025.06.27.661975; doi: https://

Insert

ymTq2-ymScarletl

Expression

Yeast

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pT2/shCol1a1/Scarlet_Seq1.3

Plasmid

#206137

Purpose

Knockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

Depositor

Maria Castro

Article

Comba et al Nat Commun. 2022 Jun 24;13(1):3606. doi:

Insert

COL1A1 (Col1a1 Mouse)

Expression

Mammalian

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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ptHe-Tub3-mScarlet

Plasmid

#201735

Purpose

Tardigrade (Hypsibius exemplaris) gamma-tubulin Tub3 tagged with mScarlet for expression in mammalian cells

Depositor

Stanislav Vinopal

Article

Novotna Floriancicova et al Sci Rep. 2023 Mar 30;13(1):5194. doi: 10

Insert

Tubulin gamma

Tags

mScarlet

Expression

Mammalian

Promoter

CMV

Available Since

July 19, 2023

Availability

Academic Institutions and Nonprofits only

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ptHe-Tub2D4-mScarlet

Plasmid

#201734

Purpose

Tardigrade (Hypsibius exemplaris) beta-tubulin Tub2D4 tagged with mScarlet for expression in mammalian cells

Depositor

Stanislav Vinopal

Article

Novotna Floriancicova et al Sci Rep. 2023 Mar 30;13(1):5194. doi: 10

Insert

Tubulin Beta D4

Tags

mScarlet

Expression

Mammalian

Promoter

CMV

Available Since

July 19, 2023

Availability

Academic Institutions and Nonprofits only

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ptHe-Tub2D3-mScarlet

Plasmid

#201733

Purpose

Tardigrade (Hypsibius exemplaris) beta-tubulin Tub2D3 tagged with mScarlet for expression in mammalian cells

Depositor

Stanislav Vinopal

Article

Novotna Floriancicova et al Sci Rep. 2023 Mar 30;13(1):5194. doi: 10

Insert

Tubulin Beta D3

Tags

mScarlet

Expression

Mammalian

Promoter

CMV

Available Since

July 19, 2023

Availability

Academic Institutions and Nonprofits only

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ptHe-Tub2D2-mScarlet

Plasmid

#201732

Purpose

Tardigrade (Hypsibius exemplaris) beta-tubulin Tub2D2 tagged with mScarlet for expression in mammalian cells

Depositor

Stanislav Vinopal

Article

Novotna Floriancicova et al Sci Rep. 2023 Mar 30;13(1):5194. doi: 10

Insert

Tubulin Beta D2

Tags

mScarlet

Expression

Mammalian

Promoter

CMV

Available Since

July 19, 2023

Availability

Academic Institutions and Nonprofits only

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