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We narrowed to 1,815 results for: cas12a

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Showing: 621 - 640 of 1815 results

  2. pET-28b-T7-hAsCas12a-enRR(E174R/S542R/K607R)-NLS(nucleoplasmin)-6xHis (AAS1902)

    Plasmid
    #114077
    Purpose
    T7 promoter bacterial expression plasmid for human codon optimized AsCas12a-enRR(E174R/S542R/K607R) with C-terminal NLS and His-tag
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Publication
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized AsCas12a-enRR (E174R/S542R/K607R) with C-terminal NLS and His-tag
    Tags
    NLS(nucleoplasmin) and 6xHis
    Expression
    Bacterial
    Mutation
    E174R/S542R/K607R
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    114077-plasmid-map-sequence-id-220525

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  3. pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdAsCas12a(D908A)-gs-UGI-NLS(SV40)(AsBE1.4) (RTW1008)

    Plasmid
    #114080
    Purpose
    CAG promoter expression plasmid for human codon optimized AsBE1.4
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Publication
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized DNase-inactive (D908A) enAsCas12a fused to N-terminal rAPOBEC1 and C-terminal UGI (AsBE1.4)
    Tags
    2x SV40 NLS and SV40 NLS
    Expression
    Mammalian
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114080-plasmid-map-sequence-id-230482

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  5. JG676: CAG-human dLbCpf1(D832A)-NLS-3xHA-3xFLAG-DmrA(X2)

    Plasmid
    #104569
    Purpose
    Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to two DmrA domains
    Depositor
    Keith Joung
    Publication
    Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dLbCpf1(D832A)-DmrA(x2)
    Tags
    3x FLAG, 3x HA, and NLS
    Expression
    Mammalian
    Mutation
    D832A
    Promoter
    CAG
    Available since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    104569-plasmid-map-sequence-id-201433

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  6. JG674: CAG-human dLbCpf1(D832A)-NLS-3xHA-3xFLAG-DmrA(X1)

    Plasmid
    #104568
    Purpose
    Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to one DmrA domain
    Depositor
    Keith Joung
    Publication
    Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dLbCpf1(D832A)-DmrA(x1)
    Tags
    3x FLAG, 3x HA, and NLS
    Expression
    Mammalian
    Mutation
    D832A
    Promoter
    CAG
    Available since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    104568-plasmid-map-sequence-id-201431

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  7. pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdenAsCas12a(E174R/S542R/K548R/D908A)-gs-UGI-NLS(SV40)(enAsBE1.4) (RTW1028)

    Plasmid
    #114084
    Purpose
    CAG promoter expression plasmid for human codon optimized enAsBE1.4
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Publication
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to N-terminal rAPOBEC1 and C-terminal UGI (enAsBE1.4)
    Tags
    2x SV40 NLS and SV40 NLS
    Expression
    Mammalian
    Mutation
    E174R, S542R, K548R and D908A, human codon optimi…
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    114084-plasmid-map-sequence-id-230536

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  9. pCAG-rAPOBEC1-gs-XTEN-gs-hdLbCas12a(D832A)-NLS(nucleoplasmin)-gs-UGI-NLS(SV40)(LbBE1.1) (RTW1295)

    Plasmid
    #114085
    Purpose
    CAG promoter expression plasmid for human codon optimized LbBE1.1
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Publication
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized DNase-inactive (D832A) LbCas12a fused to N-terminal rAPOBEC1 and C-terminal UGI (LbBE1.1)
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    D832A
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114085-plasmid-map-sequence-id-230653

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  10. pET-28b-T7-hAsCas12a-RR(S542R/K607R)-NLS(nucleoplasmin)-6xHis (AAS1916)

    Plasmid
    #114076
    Purpose
    T7 promoter bacterial expression plasmid for human codon optimized AsCas12a-RR(S542R/K607R) with C-terminal NLS and His-tag
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Publication
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized AsCas12a-RR (S542R/K607R) with C-terminal NLS and His-tag
    Tags
    NLS(nucleoplasmin) and 6xHis
    Expression
    Bacterial
    Mutation
    S542R and K607R
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114076-plasmid-map-sequence-id-220531

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  11. pET-28b-T7-hAsCas12a(E174R/S542R)-NLS(nucleoplasmin)-6xHis (AAS1880)

    Plasmid
    #114071
    Purpose
    T7 promoter bacterial expression plasmid for human codon optimized AsCas12a(E174R/S542R) with C-terminal NLS and His-tag
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Publication
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized AsCas12a (E174R/S542R) with C-terminal NLS and His-tag
    Tags
    NLS(nucleoplasmin) and 6xHis
    Expression
    Bacterial
    Mutation
    E174R and S542R
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114071-plasmid-map-sequence-id-220509

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  12. pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdenAsCas12a(E174R/S542R/K548R/D908A)-NLS(nucleoplasmin)-gs-UGI-NLS(SV40)(enAsBE1.2)

    Plasmid
    #114082
    Purpose
    CAG promoter expression plasmid for human codon optimized enAsBE1.2, also called RTW1348
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Publication
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to N-terminal rAPOBEC1 and C-terminal UGI (enAsBE1.2)
    Tags
    2x SV40 NLS and SV40 NLS
    Expression
    Mammalian
    Mutation
    E174R, S542R, K548R and D908A, human codon optimi…
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    114082-plasmid-map-sequence-id-220504

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  14. pTE4567

    Plasmid
    #107557
    Purpose
    Expresses human codon optimized inactive MbCpf1(dead2) in mammalian cells.
    Depositor
    Ervin Welker
    Publication
    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hMbCpf1(dead2)
    Tags
    3xHA and NLS
    Expression
    Mammalian
    Mutation
    E1080A
    Promoter
    CMV
    Available since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    107557-plasmid-map-sequence-id-207247

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  15. pTE3320

    Plasmid
    #107529
    Purpose
    Expresses human codon optimized LbCpf1(RVR mutant) in mammalian cells.
    Depositor
    Ervin Welker
    Publication
    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hLbCpf1(RVR)
    Tags
    3xHA and NLS
    Expression
    Mammalian
    Mutation
    G532R, K538V, Y542R
    Promoter
    CMV
    Available since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    107529-plasmid-map-sequence-id-207214

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  17. pTE3331

    Plasmid
    #107536
    Purpose
    Expresses Fn crRNA and human codon optimized FnCpf1(RVR mutant) in mammalian cells.
    Depositor
    Ervin Welker
    Publication
    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510
    Inserts
    Fn crRNA
    hFnCpf1(RVR mutant)
    Tags
    3xHA and NLS
    Expression
    Mammalian
    Mutation
    N607R, K613V, N617R
    Promoter
    CMV and human U6
    Available since
    March 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    107536-plasmid-map-sequence-id-207338

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  18. pTE3336

    Plasmid
    #107541
    Purpose
    Expresses Mb crRNA and human codon optimized MbCpf1(RR mutant) in mammalian cells.
    Depositor
    Ervin Welker
    Publication
    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510
    Inserts
    Mb crRNA
    hMbCpf1(RR mutant)
    Tags
    3xHA and NLS
    Expression
    Mammalian
    Mutation
    N576R, K637R
    Promoter
    CMV and human U6
    Available since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    107541-plasmid-map-sequence-id-207245

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  19. pTE3333

    Plasmid
    #107540
    Purpose
    Expresses Fn crRNA and human codon optimized FnCpf1(RR mutant) in mammalian cells.
    Depositor
    Ervin Welker
    Publication
    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510
    Inserts
    Fn crRNA
    hFnCpf1(RR mutant)
    Tags
    3xHA and NLS
    Expression
    Mammalian
    Mutation
    N607R, K671R
    Promoter
    CMV and human U6
    Available since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    107540-plasmid-map-sequence-id-207239

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  20. U6-5'+3' MS2-CircRNA

    Plasmid
    #213752
    Purpose
    For circular RNA-mediated prime editor using U6-5'+3' MS2-CircRNA in HEK293T cells
    Depositor
    Caixia Gao
    Publication
    Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    5' ribozyme, 5' ligation sequences, 5' MS2, 3' MS2, 3' ligation sequences, 3' ribozyme
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Feb. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    213752-plasmid-map-sequence-id-422189

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Showing: 621 - 640 of 1815 results