We narrowed to 12,282 results for: LEA

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pLKO_AU1.HA.V5_mCherry-NLS

Plasmid

#178221

Purpose

Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

Depositor

Brian Brown

Publication

Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

Insert

nPC Tagged mCherry-NLS

Use

Lentiviral

Tags

AU1.HA.V5 and Nuclear Localization Signal

Promoter

eF1a

Available since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO_AU1.HA.Ollas_mCherry-NLS

Plasmid

#178218

Purpose

Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

Depositor

Brian Brown

Publication

Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

Insert

nPC Tagged mCherry-NLS

Use

Lentiviral

Tags

AU1.HA.Ollas and Nuclear Localization Signal

Promoter

eF1a

Available since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO_AU1.C.Ollas_mCherry-NLS

Plasmid

#178212

Purpose

Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

Depositor

Brian Brown

Publication

Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

Insert

nPC Tagged mCherry-NLS

Use

Lentiviral

Tags

AU1.C.Ollas and Nuclear Localization Signal

Promoter

eF1a

Available since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pXCX-sGFAP-LMO-IRES-tdTomato

Plasmid

#178312

Purpose

Express the bacterial lactate 2-monooxygenase (LMO) enzyme in astrocytes

Depositor

Sergey Kasparov

Publication

Vaccari Cardoso et al Brain Sci. 2021 Aug 10;11(8). pii: brain

Insert

Lactate 2-monooxygenase

Use

Adenoviral

Tags

tdTomato

Expression

Mammalian

Promoter

sGFAP

Available since

Jan. 19, 2022

Availability

Academic Institutions and Nonprofits only

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His-SUMO- flag-hDDI2-D252N_pET11a

Plasmid

#169123

Purpose

expression of protein

Depositor

Barbara Dirac-Svejstrup

Publication

Dirac-Svejstrup et al Mol Cell. 2020 Jul 16;79(2):332-341.e7.

Insert

hDDI (DDI2 Human)

Expression

Bacterial

Mutation

D252N

Available since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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IBMc097

Plasmid

#161945

Purpose

Contains Type IIS restriction site free elements for IBM staging vectors. Staging plasmid for IBM on mCherry

Depositor

Baojun Wang

Publication

Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

Insert

pSB1C3(BsmBI-)-BsaI-mCherry*(27-218)-BsaI

Expression

Bacterial

Available since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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API5-CRISPR

Plasmid

#157664

Purpose

Expresses Cas9 and sgRNA targeting API5

Depositor

Byung Il Lee

Publication

Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

Insert

Apoptosis inhibitor 5 (API5 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pLY103

Plasmid

#130937

Purpose

Engineered sgRNA-LEA1 (2 BoxB aptamers) generator circuit including promoter Plux2

Depositor

Baojun Wang

Publication

Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

Insert

sgRNA-LEA1

Use

Synthetic Biology

Expression

Bacterial

Promoter

Plux2

Available since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pTEI166

Plasmid

#126075

Purpose

Vector for expression of mitochondria targeted improved YFP1-10 (T65G, T205S, T203Y) in plant cell

Depositor

Ralf-Bernd Klösgen

Publication

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Insert

mitochondria Rieske iron sulphur protein (1-100 aa)

Use

Binary

Tags

YFP1-10 (improved)

Expression

Plant

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pTEI128

Plasmid

#126209

Purpose

Module 1 vector of PlaMiNGo toolkit for cloning of candidate gene at N-terminus of GFP11x3 and expression in plant cell

Depositor

Ralf-Bernd Klösgen

Publication

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Inserts

ccdB Cassette (BsaI)
GFP11x3 (5 aa linker)

Use

Binary, module 1

Expression

Plant

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pNOC-CRISPR-35S

Plasmid

#99861

Purpose

Expresses Cas9-Nlux and sgRNA. Contains CEN/ARS and 35 terminator. No selection marker.

Depositor

Eva Farre

Publication

Poliner et al ACS Synth Biol. 2018 Apr 20;7(4):962-968

Type

Empty backbone

Use

Algae, nannochloropsis expression

Tags

Cas9-Nlux

Promoter

Ribi promoter

Available since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pEAHISMRSA2676

Plasmid

#96970

Purpose

expression of methicillin-resistant Staphylococcus aureus orf 2676

Depositor

Huanting Liu, James Naismith

Publication

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

Sar2676

Tags

N-ter TEV protease cleavable 6His

Mutation

none

Promoter

T7

Available since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pEAHISMRSAMVAK

Plasmid

#97000

Purpose

expression of methicillin-resistant Staphylococcus aureus MVAK protein

Depositor

Huanting Liu, James Naismith

Publication

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

Mevalonate kinase

Tags

N-ter TEV protease cleavable 6His

Mutation

none

Promoter

T7

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pEMS1937

Plasmid

#79782

Purpose

High copy number pUC vector containing Ple260 (PAX6 MiniPromoter) insert

Depositor

Elizabeth Simpson

Publication

Hickmott et al Mol Ther Methods Clin Dev. 2016 Aug 10;3

Insert

Ple260

Use

Pleiades promoter project [sic, pleaides plieades]

Promoter

Ple260 (PAX6 MiniPromoter)

Available since

Sept. 14, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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JW630

Plasmid

#64807

Purpose

TCP4 expression in yeast

Depositor

Detlef Weigel

Publication

Rubio-Somoza et al Curr Biol. 2014 Nov 17;24(22):2714-9. do

Insert

TCP4 (TCP4 Mustard Weed)

Tags

GAL4 Activation domain and HA

Available since

Aug. 26, 2015

Availability

Academic Institutions and Nonprofits only

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pICSL80037

Plasmid

#68260

Purpose

Level 0 Golden Gate Part: Coding sequence, neomycin phophotransferase II (Escherichia coli)

Depositor

Nicola Patron

Publication

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Coding sequence, neomycin phophotransferase II (Escherichia coli)

Use

Synthetic Biology

Available since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pICSL11061

Plasmid

#68255

Purpose

Level 1 Golden Gate Cassette: sgRNA cassette targeting BolC.GA4a-1 in Brassica oleracea

Depositor

Nicola Patron

Publication

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Promoter_AtU6-26 + sgRNA_BolC.GA4a-1

Use

Synthetic Biology

Expression

Plant

Available since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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Prp2-pRSETA

Plasmid

#61042

Purpose

Encodes the yeast Prp2 protein for overexpression and purification using a C-terminal hexahistidine tag.

Depositor

Nils Walter

Publication

Krishnan et al Nat Struct Mol Biol. 2013 Dec;20(12):145

Insert

Prp2

Tags

Histidine Tag

Expression

Bacterial

Promoter

T7

Available since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only
LI B-C N-NLS KOZ c2

Plasmid

#54443

Depositor

Martin Parniske

Publication

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

Nuclear localization signal (NLS) of SV40 large T antigen

Use

Cloning vector

Available since

Nov. 20, 2014

Availability

Academic Institutions and Nonprofits only

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SDC3_L (OZ595)

Plasmid

#35235

Depositor

Keith Joung

Publication

Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

Insert

Zinc finger array targeting SDC3

Use

Zebrafish targeting

Available since

March 15, 2012

Availability

Academic Institutions and Nonprofits only