We narrowed to 16,624 results for: puromycin

1. pLVX-M-puro

   Plasmid

   #125839

   Purpose

   modifying MCS, replacing EcoRI to BamHI region with BamHI and EcoRI

   Depositor

   Boyi Gan

   Article

   Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

   Type

   Empty backbone

   Use

   Lentiviral

   Available Since

   June 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCW-OVOL2-HA

   Plasmid

   #184737

   Purpose

   Doxycycline-inducible lentiviral expression of OVOL2

   Depositor

   Alessia Ciarrocchi, Gloria Manzotti

   Article

   Gugnoni et al J Exp Clin Cancer Res. 2022 Mar 25;41(1)

   Insert

   ovo like zinc finger 2 (OVOL2 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Promoter

   Tet ON

   Available Since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLJC5-Tmem192-3xHA

   Plasmid

   #102930

   Purpose

   Lentiviral expression of a lysosomal tag (Tmem192-HA)

   Depositor

   David Sabatini

   Article

   Abu-Remaileh et al Science. 2017 Nov 10;358(6364):807-813.

   Insert

   Tmem192 (TMEM192 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Promoter

   ubc

   Available Since

   Nov. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. lenti sgRNA(MS2)_puro optimized backbone

   Plasmid

   #73797

   Purpose

   optimized lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

   Depositor

   Feng Zhang

   Article

   Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6 and EF1A

   Available Since

   March 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMV_AA050

   Plasmid

   #216107

   Purpose

   gDNA expression for Cas9 with ChenF+E trRNA (guide only)

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Insert

   gDNA expression for Cas9 with ChenF+E trRNA
   

   Use

   CRISPR and Lentiviral; Assembled vector

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTet-O-NEUROD1-T2A-PuroR

   Plasmid

   #162338

   Purpose

   Lentiviral expression of NEUROD1 under the control of the TetON promoter

   Depositor

   Charles Gersbach

   Article

   Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

   Insert

   NEUROD1 (NEUROD1 Human)
   

   Use

   Lentiviral

   Tags

   T2A-PuroR

   Expression

   Mammalian

   Promoter

   Tet-ON

   Available Since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBP Omomer

   Plasmid

   #113169

   Purpose

   Retroviral vector for expressing Omomer (tamoxifen inducible Omomyc), by DNA transfection or retroviral infection.

   Depositor

   Sergio Nasi

   Article

   Soucek et al Cancer Res. 2002 Jun 15;62(12):3507-10.

   Insert

   Omomer: a fusion contruct of Omomyc and MER (a mutant estrogen receptor, reponsive to tamoxifen) (MYC Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   E57T, E64I, R70Q, R71N

   Available Since

   Aug. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLVX-mCherry-KATNA1

   Plasmid

   #180650

   Purpose

   Lentiviral expression vector for KATNA1. Used for generating cell lines. Has N-terminal mCherry tag. Dox-inducible. Internal ID: WISP20-47.

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   KATNA1 (KATNA1 Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   siRNA resistant to: GGACAGCACUCCCUUGAAA

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. LentiGuide_DKO

   Plasmid

   #183193

   Purpose

   Plasmid with two U6 promoters and two gRNA scaffolds that allows for inserting two gRNAs for combinatorial CRISPR Screen or double-knock-out (DKO) screen

   Depositor

   Lang Li

   Article

   Tang et al STAR Protoc. 2022 Aug 24;3(3):101556. do

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6, mU6

   Available Since

   Aug. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. T7-VEE-GFP

    Plasmid

    #58977

    Purpose

    expression of GFP using a self-replicating Venezuelan equine encephalitis (VEE) virus RNA replicon

    Depositor

    Steven Dowdy

    Article

    Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

    Insert

    EGFP
    

    Use

    Venezuelan equine encephalitis (vee) virus rna re…

    Expression

    Mammalian

    Promoter

    26S subgenomic promoter

    Available Since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHAGE_puro

    Plasmid

    #118692

    Purpose

    Empty vector to express overexpression cDNA

    Depositor

    Christopher Vakoc

    Article

    Lu et al Cancer Cell. 2018 Dec 10;34(6):970-981.e

    Type

    Empty backbone

    Use

    Lentiviral

    Promoter

    CMV

    Available Since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. PRDM9(KRAB-SSXRD-PR/SET-ZF1-dC)-Cas9

    Plasmid

    #184464

    Purpose

    Plasmid encoding PRDM9-Cas9 fusion under CMV promoter

    Depositor

    Jennifer Doudna

    Article

    Chen et al Proc Natl Acad Sci U S A. 2022 Dec 6;119

    Insert

    PRDM9(KRAB-SSXRD-PR/SET-ZF1-dC)-Cas9 (PRDM9 Human)
    

    Use

    CRISPR

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. OPA1-isoform1

    Plasmid

    #70173

    Purpose

    Mammalian expression of hsOPA1 isoform 1

    Depositor

    David Chan

    Article

    Song et al J Cell Biol. 2007 Aug 27;178(5):749-55.

    Insert

    hsOPA1 isoform 1 (OPA1 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    July 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro

    Plasmid

    #71236

    Purpose

    Express sgRNA and dCas9-KRAB from lentiviral vector

    Depositor

    Charles Gersbach

    Article

    Thakore et al Nat Methods. 2015 Dec;12(12):1143-9. doi

    Inserts

    humanized dCas9-KRAB T2A Puro
    sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Flag

    Mutation

    D10A and H840A

    Available Since

    Dec. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pXL010-Wnt dual (GFP-Fire) reporter

    Plasmid

    #40588

    Depositor

    Sean Palecek

    Article

    Azarin et al Biomaterials. 2012 Mar;33(7):2041-9. Epu

    Insert

    Wnt reporter sequences
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    Wnt promoter

    Available Since

    May 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLJC5-Tmem192-2xFlag

    Plasmid

    #102929

    Purpose

    Lentiviral expression of a lysosomal tag (Tmem192-Flag)

    Depositor

    David Sabatini

    Article

    Abu-Remaileh et al Science. 2017 Nov 10;358(6364):807-813.

    Insert

    Tmem192 (TMEM192 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Promoter

    ubc

    Available Since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLX307-VHL-3F-BirA

    Plasmid

    #220147

    Purpose

    Expresses VHL-BirA for VHL substrate identification by E-STUB

    Depositor

    William Sellers

    Article

    Huang et al Nat Chem Biol. 2024 Mar 21. doi: 10.1038

    Insert

    von Hippel-Lindau tumor suppressor (VHL Human)
    

    Use

    Lentiviral

    Tags

    3F-BirA

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    EF-1a

    Available Since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMK381 (AAVS1 CMV-OsTIR1F74G)

    Plasmid

    #140536

    Purpose

    AAVS1 CMV-OsTIR1(F74G)

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    CMV-OsTIR1(F74G)
    

    Expression

    Mammalian

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. OPA1-isoform5

    Plasmid

    #70177

    Purpose

    Mammalian expression of hsOPA1 isoform 5

    Depositor

    David Chan

    Article

    Song et al J Cell Biol. 2007 Aug 27;178(5):749-55.

    Insert

    hsOPA1 isoform 5 (OPA1 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    May 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMT525

    Plasmid

    #131392

    Purpose

    L1RP retrotransposition reporter

    Depositor

    Kathleen Burns

    Article

    Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

    Insert

    L1RP GFP-AI
    

    Expression

    Mammalian

    Available Since

    June 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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