We narrowed to 10,873 results for: kars;

1. pEGFP-C2-DR

   Plasmid

   #216266

   Purpose

   Dox-responsive module fused with EGFP

   Depositor

   Susumu Tomita

   Article

   Park et al Cell Rep. 2023 Aug 29;42(8):112826. doi:

   Insert

   Codon-optimized TetR - Lipid binding motif (KRf - KLK) - Codon-optimized TetR
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEGFP-C2-KRf-TetR

   Plasmid

   #216269

   Purpose

   Construct 2: Lipid binding domain KRφ - TetR

   Depositor

   Susumu Tomita

   Article

   Park et al Cell Rep. 2023 Aug 29;42(8):112826. doi:

   Insert

   Lipid binding motif (KRf) - TetR
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTSK573

   Plasmid

   #190881

   Purpose

   For C-terminal -Halo Tagging of a gene of interest with TRP1 selection marker in yeast S. cerevisiae

   Depositor

   Gunjan Mehta, Tatiana Karpova

   Article

   Podh et al STAR Protoc. 2022 Dec 16;3(4):101900. do

   Insert

   HaloTag
   

   Use

   Bacterial cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA5/FRT/TO/TARG1

   Plasmid

   #172580

   Purpose

   For generation of FlpIn lines with untagged TARG1

   Depositor

   Karla Feijs

   Article

   Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

   Insert

   TARG1 (OARD1 Human)
   

   Use

   Tags

   none

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDONR/zeo-TARG1

   Plasmid

   #172574

   Purpose

   For gateway cloning of N-terminal tagged TARG1 constructs.

   Depositor

   Karla Feijs

   Article

   Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

   Insert

   TARG1 (OARD1 Human)
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV(shRNA)-Puro-6 CCEPR Vb2

   Plasmid

   #108278

   Purpose

   shRNA for CCEPR lncRNA

   Depositor

   Karl Munger

   Article

   Sharma et al Virology. 2018 Feb 7;518:8-13. doi: 10.1

   Insert

   CCEPR (CCEPR Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   April 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pNCMV HPV8 E6 R138S

   Plasmid

   #87659

   Purpose

   Flag/HA tagged HPV8 E6 R138S

   Depositor

   Karl Munger

   Article

   Meyers et al PLoS Pathog. 2017 Jan 20;13(1):e1006171.

   Insert

   HPV 8E6
   

   Use

   Tags

   Flag and HA

   Expression

   Mammalian

   Mutation

   R138S

   Promoter

   CMV

   Available since

   June 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pNCMV HPV8 E6 K64A

   Plasmid

   #87656

   Purpose

   Flag/HA tagged HPV8 E6 K64A

   Depositor

   Karl Munger

   Article

   Meyers et al PLoS Pathog. 2017 Jan 20;13(1):e1006171.

   Insert

   HPV 8E6
   

   Use

   Tags

   Flag and HA

   Expression

   Mammalian

   Mutation

   K64A

   Promoter

   CMV

   Available since

   March 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pNCMV HPV8 E6 K136N

   Plasmid

   #87657

   Purpose

   Flag/HA tagged HPV8 E6 K136N

   Depositor

   Karl Munger

   Article

   Meyers et al PLoS Pathog. 2017 Jan 20;13(1):e1006171.

   Insert

   HPV 8E6
   

   Use

   Tags

   Flag and HA

   Expression

   Mammalian

   Mutation

   K136N

   Promoter

   CMV

   Available since

   March 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pNCMV HPV8 E6 K142A

    Plasmid

    #87660

    Purpose

    Flag/HA tagged HPV8 E6 K142A

    Depositor

    Karl Munger

    Article

    Meyers et al PLoS Pathog. 2017 Jan 20;13(1):e1006171.

    Insert

    HPV 8E6
    

    Use

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    K142A

    Promoter

    CMV

    Available since

    March 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pNCMV HPV8 E6 L59D

    Plasmid

    #87655

    Purpose

    Flag/HA tagged HPV8 E6 L59D

    Depositor

    Karl Munger

    Article

    Meyers et al PLoS Pathog. 2017 Jan 20;13(1):e1006171.

    Insert

    HPV 8E6
    

    Use

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    L59D

    Promoter

    CMV

    Available since

    March 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pNCMV MmuPV1 E6 R130A

    Plasmid

    #87654

    Purpose

    Flag/HA tagged MmuPV1 E6 R130A

    Depositor

    Karl Munger

    Article

    Meyers et al PLoS Pathog. 2017 Jan 20;13(1):e1006171.

    Insert

    MmuPV1 E6
    

    Use

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    R130A

    Promoter

    CMV

    Available since

    March 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pED54pRS306 pRPS2-Ste7DS NLS(4E)-mCherry

    Plasmid

    #68276

    Purpose

    SKARS: Fus3 and Kss1 MAPK activity sensor: Ste7DS 2xNLS(4E) mCherry Non functional sensor with phospho-mimicking NLS

    Depositor

    Serge Pelet

    Article

    Durandau et al BMC Biol. 2015 Aug 1;13:55. doi: 10.1186

    Insert

    Ste7DS NLS(4E) mCherry
    

    Use

    Tags

    mCherry

    Expression

    Yeast

    Mutation

    Promoter

    RPS2

    Available since

    March 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pED55 pRS306 pRPS2-SteND-NLS-mCherry

    Plasmid

    #68277

    Purpose

    SKARS: Fus3 and Kss1 MAPK activity sensor: Ste7ND 2xNLS mCherry Non functional sensor with non-docking mutation

    Depositor

    Serge Pelet

    Article

    Durandau et al BMC Biol. 2015 Aug 1;13:55. doi: 10.1186

    Insert

    Ste7ND NLS mCherry
    

    Use

    Tags

    mCherry

    Expression

    Yeast

    Mutation

    Promoter

    pRPS2

    Available since

    Sept. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pED53 pRS306 pRPS2-Ste7DS NLS(4A)-mCherry

    Plasmid

    #68275

    Purpose

    SKARS: Fus3 and Kss1 MAPK activity sensor: Ste7DS 2xNLS(4A) mCherry Non functional sensor with non-phosphorylatable NLS

    Depositor

    Serge Pelet

    Article

    Durandau et al BMC Biol. 2015 Aug 1;13:55. doi: 10.1186

    Insert

    Ste7DS NLS(4A) mCherry
    

    Use

    Tags

    mCherry

    Expression

    Yeast

    Mutation

    Promoter

    pRPS2

    Available since

    Sept. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSG5-(nic)p85

    Plasmid

    #55720

    Purpose

    eukaryotic expression of both SH2 and the inter-SH2 domain of murine p85α.

    Depositor

    Julian Downward

    Article

    Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

    Insert

    p85α
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    SH2 and the inter-SH2 domain

    Promoter

    T7

    Available since

    Sept. 30, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pCold TDP43 WT

    Plasmid

    #27451

    Depositor

    Aaron Gitler

    Article

    Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Tags

    Factor Xa, GST, and His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. SBFP2-anti-AlfaTag nanobody

    Plasmid

    #171826

    Purpose

    Bacterial expression vector for production of fluorescent anti-AlfaTag nanobody

    Depositor

    Ikuo Wada

    Article

    Matsumoto et al J Biol Chem. 2021 Jul;297(1):100851. doi

    Insert

    SBFP2-anti-AlfaTag nanobody
    

    Use

    Tags

    6xHis and SEP tag (6xD)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDB.His.MBP.3C CARD8 CARD WT

    Plasmid

    #164022

    Purpose

    Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 UPA-CARD

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Tags

    His6-MBP-3C

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLenti-EF1a-hChR2(H134R)-EYFP-WPRE

    Plasmid

    #20942

    Purpose

    3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to EYFP driven by EF1a promoter for optogenetic activation

    Depositor

    Karl Deisseroth

    Article

    Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

    Insert

    channelrhodopsin-2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    H134R mutation in ChR2

    Promoter

    EF1alpha

    Available since

    May 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. mCh-alpha-tubulin

    Plasmid

    #49149

    Purpose

    expression of alpha-tubulin fused to mCherry. Used for visualization of microtubules.

    Depositor

    Gia Voeltz

    Article

    Friedman et al J Cell Biol. 2010 Aug 9;190(3):363-75. d

    Insert

    alpha tubulin (TUBA1B Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pTRE3G-EGFP-IRES(Neo)R

    Plasmid

    #158068

    Purpose

    eGFP under the TRE3G promoter, Neo resistance. For cloning GOI

    Depositor

    Adolfo Rivero-Muller

    Article

    Michalec-Wawiorka et al Int J Mol Sci. 2021 May 14;22(10). pii:

    Insert

    eGFP
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    Aug. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. cfSGFP2-anti-AlfaTag nanobody

    Plasmid

    #171818

    Purpose

    Bacterial expression vector for production of fluorescent anti-AlfaTag nanobody

    Depositor

    Ikuo Wada

    Article

    Matsumoto et al J Biol Chem. 2021 Jul;297(1):100851. doi

    Insert

    cfSGFP2-anti-AlfaTag nanobody
    

    Use

    Tags

    6xHis and SEP tag (6xD)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3.1 LIC 6A CARD8-FLAG

    Plasmid

    #169948

    Purpose

    Mammalian expression of CARD8-FLAG

    Depositor

    Hao Wu

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAAV-Ef1a-DIO ChETA-EYFP

    Plasmid

    #26968

    Purpose

    AAV expression of humanized ChR2 with E123T/H134R mutations fused to EYFP driven by EF1a promoter for optogenetic activation

    Depositor

    Karl Deisseroth

    Article

    Gunaydin et al Nat Neurosci. 2010 Mar;13(3):387-92. doi

    Has Service

    AAV1, AAV5, and AAV9

    Insert

    hChR2(E123T/H134R)-EYFP
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    E123T, H134R

    Promoter

    EF1alpha

    Available since

    Jan. 28, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAAV-EF1a-4mtGCaMP6f-DIO

    Plasmid

    #179529

    Purpose

    Cre-dependent expression of mitochondria-targeted GCaMP6f under control of the EF1a promoter

    Depositor

    Maarten Kole

    Article

    Kole et al Nat Commun. 2022 Dec 9;13(1):7598. doi:

    Insert

    4mtGCaMP6f
    

    Use

    AAV

    Tags

    4x Cox8 targeting sequence

    Expression

    Mutation

    Promoter

    Ef1a

    Available since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. TLCV2-DelGFP

    Plasmid

    #133302

    Purpose

    EGFP reporter was removed in the TLCV2 vector so it could be used for inducible gene CRISPR knockout in cell lines with an eGFP reporter.

    Depositor

    Jingshi Shen

    Article

    Wang et al J Biol Chem. 2019 Dec 27;294(52):19988-1

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA4/TO 3xFlag-m-PKD1-2xMyc/His

    Plasmid

    #83452

    Purpose

    Full-length murine PC1 for Eukaryotic Expression (982)

    Depositor

    Thomas Weimbs

    Article

    Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

    Insert

    polycystin-1 (Pkd1 Mouse)
    

    Use

    Tags

    2xMyc, 3xFlag, and 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pmCherry-MS2coatProtein

    Plasmid

    #103832

    Purpose

    Live cell reader for MS2 loop RNA production

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    mCherry-MS2 coat protein (cp )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    MCP: nt 202-240 are missing (13 aa), C242G (A81G)…

    Promoter

    CMV

    Available since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET30a-Tn5

    Plasmid

    #127917

    Purpose

    Prokaryotic expression of the unmodified Tn5 for Illumina sequencing library preperation.

    Depositor

    Silin Zhong

    Article

    Tu et al Nat Commun. 2020 Oct 9;11(1):5089. doi:

    Insert

    Tn5
    

    Use

    Tags

    Mxe intein - Chitin-binding domain

    Expression

    Bacterial

    Mutation

    E54K, L372P

    Promoter

    T7

    Available since

    Aug. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge