We narrowed to 19,557 results for: Mut

Showing: 6421 - 6440 of 19557 results

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pET-20b-M-Ntx-S1

Plasmid

#58716

Purpose

Expresses in Escherichia coli Notexin with a N-terminal extra methionine and with the amino acid substitution N1S

Depositor

Fiorella Tonello

Article

Simonato et al Toxicon. 2014 Jun 18. pii: S0041-0101(14

Insert

Notexin

Tags

Methionine

Expression

Bacterial

Mutation

Changed Asparagine 1 to Serine

Promoter

T7

Available Since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

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OPEN-A*24:02

Plasmid

#235133

Purpose

E. coli expression of HLA-A*24:02 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin.

Depositor

Nikolaos Sgourakis

Article

Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

Insert

OPEN-HLA-A*24:02 (HLA-A Human)

Expression

Bacterial

Mutation

G120C

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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OPEN-HLA-A*01:01

Plasmid

#235134

Purpose

E. coli expression of HLA-A*01:01 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin

Depositor

Nikolaos Sgourakis

Article

Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

Insert

OPEN-HLA-A*01:01 (HLA-A Human)

Expression

Bacterial

Mutation

G120C

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-WT (AF41)

Plasmid

#21367

Depositor

Gregory Germino

Article

Qian et al Proc Natl Acad Sci U S A. 2002 Dec 24. 9

Insert

autosomal dominant polycystic kidney disease type I (PKD1 Human)

Tags

EGFP and FLAG

Expression

Mammalian

Mutation

wild-type sequence*

Available Since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only

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HsGRK6-mVenus

Plasmid

#137780

Purpose

Visualization of GRK6

Depositor

Dorus Gadella

Article

Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

Insert

Human GRK6 (GRK6 Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-GFPLaminA-L530P

Plasmid

#32710

Depositor

Wendy Bickmore

Article

Gilchrist et al BMC Cell Biol. 2004 Dec 13;5(1):46.

Insert

Lamin A (LMNA Human)

Tags

GFP

Expression

Mammalian

Mutation

L530P

Promoter

CMV

Available Since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only
pENTR_L1-Turbo-NES-YFP-STOP-L2

Plasmid

#127358

Purpose

gateway entry vector for expressing cytosolic TurboID-YFP under a promoter of choice

Depositor

Dominique Bergmann

Article

Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

Insert

TurboID (BirA mutant)

Use

Gateway entry vector

Tags

GS linker, NES, V5, and YFP

Mutation

Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

Promoter

no promoter

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pUC57-sgRNA-MS2-U6

Plasmid

#171694

Purpose

To construct expression vector for tBE-V5-mA3

Depositor

Jia Chen

Article

Wang et al Nat Cell Biol. 2021 May 10. pii: 10.1038

Insert

sgRNA scaffold-MS2-U6

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 9, 2021

Availability

Academic Institutions and Nonprofits only

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FKBP-CD28TMD-NTEVp(H75E)

Plasmid

#137829

Purpose

MESA split-TEV protease chain with FKBP Rapamycin ectodomain, CD28 transmembrane domain, N-terminal H75E mutant TEV protease

Depositor

Joshua Leonard

Article

Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

Insert

FKBP-CD28TMD-NTEVp(H75E)

Tags

3x FLAG

Expression

Mammalian

Mutation

Mutated Histidine 75 to Glutamic Acid in NTEVp fr…

Promoter

CMV

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pAG397

Plasmid

#226733

Purpose

Expresses a reporter for an alpha-helical OMM protein

Depositor

Jonathan Weissman

Article

Guna et al Science. 2022 Oct 21;378(6617):317-322.

Insert

OMP25 (SYNJ2BP Human)

Use

Lentiviral

Tags

GFP11

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pCRISPEY-GAL-Kan-ADE2

Plasmid

#232105

Purpose

Expresses galactose-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.

Depositor

Jeffrey Lewis

Article

Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

Insert

ADE2 gRNA and repair template

Use

CRISPR

Expression

Yeast

Mutation

Repair template introduces C at nt 466 of ADE2 to…

Promoter

GAL7

Available Since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pET21a Ce-iPGM-bio

Plasmid

#162559

Purpose

Expresses C. elegans iPGM containing a C-terminal biotinylation site and His tag

Depositor

James Inglese

Article

Wiedmann et al J Biol Chem. 2021 Apr 1:100628. doi: 10.

Insert

C. elegans iPGM bio-6His (ipgm-1 Nematode)

Tags

6His tag, T7 tag, Thrombin cleavage site, and bio…

Expression

Bacterial

Promoter

T7

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pET21a Bm-iPGM-bio

Plasmid

#162565

Purpose

Expresses B. malayi iPGM containing a C-terminal biotinylation site and His tag

Depositor

James Inglese

Article

Wiedmann et al J Biol Chem. 2021 Apr 1:100628. doi: 10.

Insert

B. malayi iPGM bio-6His

Tags

6His tag, T7 tag, biotinylation sequence, and thr…

Expression

Bacterial

Promoter

T7

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pUASp YFP Ras85D CA

Plasmid

#52247

Purpose

Drosophila expression of YFP-Ras85D constitutively active mutant

Depositor

Matthew Scott

Insert

Ras85D (Ras85D Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

Q61L; CA mutation

Promoter

GAL4

Available Since

March 21, 2014

Availability

Academic Institutions and Nonprofits only
SPOP-V5 (BTB)

Plasmid

#169721

Purpose

Mammalian Expression of mouse SPOP-BTB (L186D, L190D, L193D, I217K)

Depositor

Tanja Mittag

Article

Bouchard et al Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi

Insert

SPOP (Spop Mouse)

Tags

V5

Expression

Mammalian

Mutation

L186D, L190D, L193D, I217K

Promoter

CMV

Available Since

June 14, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-Kdm6b(1025-End)_H1390A-pA

Plasmid

#100279

Purpose

In vitro transcription template (T7 promoter) for Kdm6b catalytic domain mutant

Depositor

Yi Zhang

Article

Inoue et al Nature. 2017 Jul 19. doi: 10.1038/nature

Insert

Kdm6b (Kdm6b Mouse)

Tags

Flag epitope tag

Expression

Mammalian

Mutation

Mutated catalytic domain (1025aa-End) (please see…

Available Since

Sept. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pFUGW-GtACR1_C102A-EYFP

Plasmid

#119196

Purpose

Step-function mutant of Guillardia theta anion channelrhodopsin 1 (GtACR1) with dramatically prolonged open state

Depositor

John Spudich

Article

Govorunova et al eNeuro. 2018 Jul 10;5(3). pii: eN-MNT-01

Insert

Anion channelrhodopsin GtACR1 step-function mutant C102A

Use

Lentiviral

Tags

EYFP

Mutation

changed cysteine 102 to alanine

Promoter

Ubiquitin

Available Since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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PTPRA-SHC

Plasmid

#139611

Purpose

StrepIII-HA-tagged gene expression

Depositor

Markku Varjosalo

Article

Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

Insert

PTPRA (PTPRA Human)

Tags

StrepIII-HA

Expression

Mammalian

Promoter

CMV

Available Since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV6-G2-ATP8-V5 (puromycin)

Plasmid

#86852

Purpose

mammalian expression of ATP8 with G2 mitochondrial targeting sequence and V5 tag

Depositor

Matthew O'Connor

Article

Boominathan et al Nucleic Acids Res. 2016 Nov 2;44(19):934

Insert

ATP8 (ATP8 Human)

Tags

ATP5G2 (G2) mitochondrial targeting signal and V5

Expression

Mammalian

Mutation

codon optimized

Promoter

CMV

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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TDP-43_CTD_A321V

Plasmid

#98671

Purpose

Expresses 6xHis-tagged C-terminal domain of TDP-43 with A321V

Depositor

Nicolas Fawzi

Article

Conicella et al Structure. 2016 Sep 6;24(9):1537-49. doi

Insert

TDP-43 (TARDBP Human)

Tags

6xHis

Expression

Bacterial

Mutation

A321V

Available Since

July 27, 2017

Availability

Academic Institutions and Nonprofits only

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