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pGL0_128 [ORI_BC_28]

Plasmid

#199023

Purpose

Level 0 part

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

ORI_BC_28

Use

Synthetic Biology

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pGLEL_3 [εω]

Plasmid

#199059

Purpose

Even assembly end-linker

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

εω assembly linker

Use

Synthetic Biology

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pGL0_122 [ORI_BC_22]

Plasmid

#199017

Purpose

Level 0 part

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

ORI_BC_22

Use

Synthetic Biology

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pGLDV_15 [38e3G]

Plasmid

#199078

Purpose

Even assembly vector with sfGFP dropout for Even level assemblies

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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sgRNA5_8xCTS-ECFP-pA

Plasmid

#200247

Purpose

Mammalian transfections; 8xCTS-ECFP reporter construct 5

Depositor

Tatjana Sauka-Spengler

Article

Pelea et al bioRxiv 2023.05.08.539738

Insert

sgRNA5_8xCTS

Expression

Mammalian

Available Since

June 15, 2023

Availability

Academic Institutions and Nonprofits only

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sgRNA3_8xCTS-ECFP-pA

Plasmid

#200245

Purpose

Mammalian transfections; 8xCTS-ECFP reporter construct 3

Depositor

Tatjana Sauka-Spengler

Article

Pelea et al bioRxiv 2023.05.08.539738

Insert

sgRNA3_8xCTS

Expression

Mammalian

Available Since

June 15, 2023

Availability

Academic Institutions and Nonprofits only

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sgRNA2_8xCTS-ECFP-pA

Plasmid

#200244

Purpose

Mammalian transfections; 8xCTS-ECFP reporter construct 2

Depositor

Tatjana Sauka-Spengler

Article

Pelea et al bioRxiv 2023.05.08.539738

Insert

sgRNA2_8xCTS

Expression

Mammalian

Available Since

June 15, 2023

Availability

Academic Institutions and Nonprofits only

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U6-TM2Emp-6T_iBlue-ModFlanks

Plasmid

#200236

Purpose

Backbone for cloning longer RNA triggers that activate iSBH-sgRNAs

Depositor

Tatjana Sauka-Spengler

Article

Pelea et al bioRxiv 2023.05.08.539738

Type

Empty backbone

Expression

Mammalian

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pBZS-YP_001498439

Plasmid

#198370

Purpose

Express YP_001498439 in bacterial cells.

Depositor

Takashi Ito

Article

Miura et al BMC Biotechnol. 2022 Nov 4;22(1):33. doi

Insert

YP_001498439

Expression

Bacterial

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD-CC-Di-C-Venus

Plasmid

#190710

Purpose

Encodes the parallel coiled-coil CC-Di followed by C-Venus.

Depositor

Nigel Savery

Article

Rhys et al Nat Chem Biol. 2022 Jul 14. pii: 10.1038

Insert

CC-Di-C-Venus

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD-C-Venus-CC-Di

Plasmid

#190712

Purpose

Encodes C-Venus followed by the parallel coiled-coil CC-Di.

Depositor

Nigel Savery

Article

Rhys et al Nat Chem Biol. 2022 Jul 14. pii: 10.1038

Insert

C-Venus-CC-Di

Available Since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pVY-16 TC-TEM1

Plasmid

#194630

Purpose

antibiotic resistance enzyme with tetracysteine tag for visualization of enzyme

Depositor

Allie Obermeyer

Article

Yeong et al Nat Commun. 2022 Dec 22;13(1):7882. doi:

Insert

tetracysteine-TEM1

Tags

MGCCPGCCGGA

Expression

Bacterial

Promoter

pBAD

Available Since

Jan. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pVY-14 TC-CAT

Plasmid

#194628

Purpose

antibiotic resistance enzyme with tetracysteine tag for visualization of enzyme

Depositor

Allie Obermeyer

Article

Yeong et al Nat Commun. 2022 Dec 22;13(1):7882. doi:

Insert

tetracysteine-CAT

Tags

MGCCPGCCGGA

Expression

Bacterial

Promoter

T7

Available Since

Jan. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pPRC

Plasmid

#192855

Purpose

Cloning of promoter of interest in front of mkate2 reporter gene

Depositor

Eveline Peeters

Article

Bernauw et al Methods Mol Biol. 2022;2516:113-141. doi

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

no promoter

Available Since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pYTK001_O2Aq(1st)

Plasmid

#190917

Purpose

2A peptide O2A cloned in yeast MoClo entry vector (pYTK001) for use as the first 2A peptide in quad-cistronic construct assembly

Depositor

Zhen Wang

Article

Mukherjee et al Biotechnol Bioeng. 2022 Sep 27. doi: 10.

Insert

O2A

Use

Synthetic Biology

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pYTK001_O2Ab

Plasmid

#183083

Purpose

2A peptide O2A cloned in yeast MoClo entry vector (pYTK001) for use in bi-cistronic and as the first 2A peptide in tri-cistronic construct assembly

Depositor

Zhen Wang

Article

Mukherjee et al Biotechnol Bioeng. 2022 Sep 27. doi: 10.

Insert

O2A

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pYTK001_P2Ab

Plasmid

#183084

Purpose

2A peptide P2A cloned in yeast MoClo entry vector (pYTK001) for use in bi-cistronic and as the first 2A peptide in tri-cistronic construct assembly

Depositor

Zhen Wang

Article

Mukherjee et al Biotechnol Bioeng. 2022 Sep 27. doi: 10.

Insert

P2A

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pWSK29-wzzpHS2

Plasmid

#172971

Purpose

pWSK29 with S. flexneri wzzpHS2 gene

Depositor

Renato Morona

Article

Murray et al J Bacteriol. 2006 Apr;188(7):2735-9. doi

Insert

wzzPHS2 gene from Shigella flexneri

Expression

Bacterial

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pEMF034

Plasmid

#157631

Purpose

sMHT-113

Depositor

Jonathan Silberg

Article

Fulk et al ACS Synth Biol. 2020 Nov 20;9(11):3104-3

Inserts

sMHT(1-113)-SYNZIP17
SYNZIP18-sMHT(114-230)

Expression

Bacterial

Available Since

Sept. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pWSK29-wzzBSF

Plasmid

#172967

Purpose

pWSK29 with S. flexneri 2a wzzBSF gene

Depositor

Renato Morona

Article

Murray et al J Bacteriol. 2006 Apr;188(7):2735-9. doi

Insert

wzzB gene from Shigella flexneri

Expression

Bacterial

Available Since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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