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pAc5.1B-EGFP-DmCaf40-V186E_AG

Plasmid

#148818

Purpose

Insect Expression of DmCaf40-V186E

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

DmCaf40-V186E (Rcd-1 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpH-DmCaf40_25-291_AF

Plasmid

#148734

Purpose

Bacterial Expression of DmCaf40_25-291

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

DmCaf40_25-291 (Rcd-1 Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmCaf40-dsRNAres_AA

Plasmid

#148288

Purpose

Insect Expression of DmCaf40-dsRNAres

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

DmCaf40-dsRNAres (Rcd-1 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmCaf40-dsRNAres_AA

Plasmid

#148290

Purpose

Insect Expression of DmCaf40-dsRNAres

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

DmCaf40-dsRNAres (Rcd-1 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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tetO-Fzd1-IRES-tdTomato (TET006)

Plasmid

#160510

Purpose

This plasmid expresses Fzd1 under the activation of tTA trans-activator along with fluorescent marker tdTomato.

Depositor

C. Ron Yu

Article

Wu et al bioRxiv 2022

Insert

Fzd1 (Fzd1 Mouse)

Expression

Mammalian

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-dLbCpf1-BE-EE

Plasmid

#154147

Purpose

Expresses dLbCpf1-BE-EE(dLbCas12a-BE-EE) in mammalian cells.

Depositor

Jin-Soo Kim

Article

Kim et al Nat Commun. 2020 Aug 13;11(1):4072. doi:

Insert

dLbCpf1-BE-EE (dCas12a-BE-EE)

Tags

SV40 NLS

Expression

Mammalian

Mutation

APOBEC1(R126E+R132E), dLbCpf1(D832A+E925A+D1148A)

Promoter

CMV

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pJH3458

Plasmid

#179573

Purpose

Pttr-39::tomm20::miniSOG-SL2::BFP unc-54 3' UTR C.elegans D-MN -specific expression of miniSOG-SL2 BFP

Depositor

Mei Zhen

Article

Gao et al Elife. 2018 Jan 23;7. pii: 29915. doi: 1

Insert

tomm20::miniSOG (tomm-20 Nematode)

Tags

BFP

Expression

Worm

Promoter

Pttr-39

Available Since

March 1, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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mCerulean3-13aa-Venus-pEGFP-C3

Plasmid

#166753

Purpose

For expression of mCerulean3 linked to Venus by 13 amino acid linker

Depositor

David Andrews

Article

Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

Insert

mCerulean3-13aa-Venus

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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mCerulean3-39aa-Venus-pEGFP-C3

Plasmid

#166755

Purpose

For expression of mCerulean3 linked to Venus by 39 amino acid linker

Depositor

David Andrews

Article

Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

Insert

mCerulean3-39aa-Venus

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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PdnaK-IR3 GFP pZa

Plasmid

#170084

Purpose

GFP expression under the control of E. coli dnaK promoter engineered with IR3 HAIR motif from M. tuberculosis

Depositor

Urartu Seker

Article

Saltepe et al ACS Synth Biol. 2019 Oct 18;8(10):2404-2

Insert

Green fluorescent protein

Use

Synthetic Biology

Expression

Bacterial

Promoter

PdnaK-IR3

Available Since

Sept. 20, 2021

Availability

Academic Institutions and Nonprofits only

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PdnaK-IR2 GFP pZa

Plasmid

#170081

Purpose

GFP expression under the control of E. coli dnaK promoter engineered with IR2 HAIR motif from M. tuberculosis

Depositor

Urartu Seker

Article

Saltepe et al ACS Synth Biol. 2019 Oct 18;8(10):2404-2

Insert

Green fluorescent protein

Use

Synthetic Biology

Expression

Bacterial

Promoter

PdnaK-IR2

Available Since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pET30 W96A-GLTP

Plasmid

#170734

Purpose

The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

Depositor

Rhoderick Brown

Article

Malinina et al Nature. 2004 Aug 26;430(7003):1048-53. d

Insert

W96A-GLTP (GLTP Human)

Tags

His

Expression

Bacterial

Mutation

W96A

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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Dcp2 ΔH1 Δ5H

Plasmid

#163582

Purpose

express Dcp2 ΔH1 Δ5H at endogenous level in S.cerevisiae

Depositor

Michael Rosen

Article

Xing et al Elife. 2020 Jun 19;9. pii: 56525. doi: 1

Insert

Dcp2 (DCP2 Budding Yeast)

Tags

GFP

Expression

Yeast

Mutation

L255A; mutate 440-450, 489-500, 701-708, 827-831,…

Promoter

DCP2 promoter

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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Dcp2C Δ5H

Plasmid

#163581

Purpose

express Dcp2C Δ5H at endogenous level in S.cerevisiae

Depositor

Michael Rosen

Article

Xing et al Elife. 2020 Jun 19;9. pii: 56525. doi: 1

Insert

Dcp2 (DCP2 Budding Yeast)

Tags

GFP

Expression

Yeast

Mutation

Truncation of aa1-300; mutate aa440-450, aa489-50…

Promoter

DCP2 promoter

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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pET-CVm-Sec22b

Plasmid

#168029

Purpose

Expresses eCFP-Sec22b(A131-H190)-mVenus in bacterial cells

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

eCFP-Sec22b(A131-H190)-mVenus

Tags

Hisx6 and myc

Expression

Bacterial

Available Since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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pET-CVm-VAMP8

Plasmid

#168028

Purpose

Expresses eCFP-VAMP8(G7-R67)-mVenus in bacterial cells

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

eCFP-VAMP8(G7-R67)-mVenus

Tags

Hisx6 and myc

Expression

Bacterial

Available Since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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CMV-mEGFP(A206K)-paCaMKII(I539E)-ShadowG

Plasmid

#167478

Purpose

Expresses paCaMKII in mammalian cells to measure structural change of paCaMKII.

Depositor

Hideji Murakoshi

Article

Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

Insert

mEGFP(A206K)-paCaMKII(I539E)-ShadowG

Tags

ShadowG and mEGFP(A206K)

Expression

Mammalian

Promoter

CMV

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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CMV-mEGFP(A206K)-paCaMKII(C450A)-ShadowG

Plasmid

#167479

Purpose

Expresses paCaMKII in mammalian cells to measure structural change of paCaMKII.

Depositor

Hideji Murakoshi

Article

Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

Insert

mEGFP(A206K)-paCaMKII(C450A)-ShadowG

Tags

ShadowG and mEGFP(A206K)

Expression

Mammalian

Promoter

CMV

Available Since

March 30, 2021

Availability

Academic Institutions and Nonprofits only

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mCerulean3-31aa-Venus-pEGFP-C3

Plasmid

#166754

Purpose

For expression of mCerulean3 linked to Venus by 31 amino acid linker

Depositor

David Andrews

Article

Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

Insert

mCerulean3-31aa-Venus

Expression

Mammalian

Promoter

CMV

Available Since

March 30, 2021

Availability

Academic Institutions and Nonprofits only

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mPLD2-PX (62-192)

Plasmid

#119128

Purpose

Bacterial expression of human phox homology (PX) domain, mPLD2-PX (62-192)

Depositor

Brett Collins

Article

Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

Insert

mPLD2-PX (62-192)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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