We narrowed to 7,669 results for: ski

1. pCAG-2dV-Camui (T286A)

   Plasmid

   #220367

   Purpose

   T286A phosphodead mutant of pCAG-2dV-Camui

   Depositor

   Ryohei Yasuda

   Article

   Jain et al Nature. 2024 Oct 9. doi: 10.1038/s41586-

   Insert

   mVenus(Y145W)-mVenus(Y145W)-rat CaMK2 alpha(T286A)-mEGFP(A206K) (Camk2a Synthetic, Rat)
   

   Tags

   mEGFP(A206K) and mVenus(Y145W)-mVenus(Y145W)

   Expression

   Mammalian

   Mutation

   changed Threonine 286 to Alanine

   Promoter

   CAG

   Available Since

   Oct. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONR223_FCGR3B_WT

   Plasmid

   #82896

   Purpose

   Gateway Donor vector containing FCGR3B, part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, Matthew Meyerson, David Root

   Article

   Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

   Insert

   FCGR3B (FCGR3B Human)
   

   Use

   Gateway entry vector

   Mutation

   N65S

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR223_CGREF1_WT

   Plasmid

   #82895

   Purpose

   Gateway Donor vector containing CGREF1, part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, Matthew Meyerson, David Root

   Article

   Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

   Insert

   CGREF1 (CGREF1 Human)
   

   Use

   Gateway entry vector

   Mutation

   deletion of AA 233-249

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDONR223_PIH1D1_WT_V5

   Plasmid

   #82943

   Purpose

   Gateway Donor vector containing PIH1D1, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, Matthew Meyerson, David Root

   Article

   Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

   Insert

   PIH1D1 (PIH1D1 Human)
   

   Use

   Gateway entry vector

   Mutation

   M9L, G10E, V224I, P287L

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. TRCN0000338399

   Plasmid

   #78156

   Purpose

   shXPO1 targeting CDS

   Depositor

   William Hahn

   Article

   Hong et al Nat Commun. 2016 Jun 22;7:11987. doi: 10

   Insert

   XPO1 (XPO1 Human)
   

   Use

   Lentiviral and RNAi

   Promoter

   hU6 and hU6

   Available Since

   June 22, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pDONR223_KEAP1_p.G524C

   Plasmid

   #82779

   Purpose

   Gateway Donor vector containing KEAP1, part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, Matthew Meyerson, David Root

   Article

   Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

   Insert

   KEAP1 (KEAP1 Human)
   

   Use

   Gateway entry vector

   Mutation

   G524C; C624S

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR223_RNH1_WT_V5

   Plasmid

   #82995

   Purpose

   Gateway Donor vector containing RNH1, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

   Depositor

   Jesse Boehm, Matthew Meyerson, David Root

   Article

   Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

   Insert

   RNH1 (RNH1 Human)
   

   Use

   Gateway entry vector

   Mutation

   R188H

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. t1-405

   Plasmid

   #166127

   Purpose

   For expression of human talin-1 head (residues 1-405) in E. coli. N-terminal His6-tag, Xpress-epitope (DLYDDDDK) and enterokinase cleavage site for tag removal.

   Depositor

   Vesa Hytönen

   Article

   Kukkurainen et al J Cell Sci. 2020 Oct 12;133(19). pii: 13

   Insert

   Tln1Head (1-405) (TLN1 Human)
   

   Tags

   His6-tag, Xpress-epitope (DLYDDDDK) and enterokin…

   Expression

   Bacterial

   Mutation

   residues 1-405 only

   Available Since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a_DmKHC[1-421]-SNAP-6xHis

   Plasmid

   #196975

   Purpose

   Bacterial expression plasmid for His tag-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal SNAP tag and C-terminal 6xHis tag

   Depositor

   Lukas Kapitein

   Article

   Deguchi et al Science. 2023 Mar 10;379(6636):1010-1015

   Insert

   Kinesin Heavy Chain (Khc Fly)
   

   Tags

   6xHis and SNAP

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   July 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pU6-pegRNA-HEK3-CTTins-px330-scaffold

    Plasmid

    #180017

    Purpose

    Transiently expressing a pegRNA to introduce HEK3 CTT insertion in human cells. It has a sgRNA scaffold from px330.

    Depositor

    Dirk Hockemeyer

    Article

    Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

    Insert

    Prime editing pegRNA for HEK3-CTTins, with px330 scaffold (EPHA8 Synthetic)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    Human U6

    Available Since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDONR223_RALA_WT_V5

    Plasmid

    #82988

    Purpose

    Gateway Donor vector containing RALA, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, Matthew Meyerson, David Root

    Article

    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

    Insert

    RALA (RALA Human)
    

    Use

    Gateway entry vector

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLVX-TetOne-FFSS-Cyclin D1 (T286A)

    Plasmid

    #215147

    Purpose

    Expresses FFSS-Cyclin D1 (T286A) in mammalian cells from an inducible lentiviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    CCND1 (CCND1 Human)
    

    Use

    Lentiviral

    Tags

    FFSS (FLAG-FLAG-STREP-STREP)

    Expression

    Mammalian

    Mutation

    With T286A mutation.

    Promoter

    TRE3GS

    Available Since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV.CAG-FLEX.iAChSnFR-Venus-NULL

    Plasmid

    #137960

    Purpose

    Expresses FLEXed iAChSnFR (non-binding yellow version) under CAG promoter

    Depositor

    Loren Looger

    Article

    Borden et al bioRxiv 2020

    Insert

    iAChSnFR (non-binding yellow variant)
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Mutation

    Y140A binding site mutation

    Promoter

    CAG

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMXs-Sox2-17

    Plasmid

    #193353

    Purpose

    Retroviral vector expressing mouse Sox2-17 chimeric transcription factor (super-Sox)

    Depositor

    Hans Schöler

    Article

    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

    Insert

    Sox2-17 (Sox2 Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    Sox2-17 is engineered highly cooperative chimeric…

    Promoter

    5'LTR

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDONR223_NFIL3_WT_V5

    Plasmid

    #82985

    Purpose

    Gateway Donor vector containing NFIL3, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, Matthew Meyerson, David Root

    Article

    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

    Insert

    NFIL3 (NFIL3 Human)
    

    Use

    Gateway entry vector

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGL3-basic-FTO promoter_WT

    Plasmid

    #108922

    Purpose

    The core promoter region of FTO was inserted into pGL3 basic vector

    Depositor

    Jianjun Chen

    Article

    Su et al Cell. 2018 Jan 11;172(1-2):90-105.e23. d

    Insert

    FTO (FTO Human)
    

    Tags

    NA

    Expression

    Mammalian

    Mutation

    NA

    Available Since

    May 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDONR223_CCL2_WT_V5

    Plasmid

    #82935

    Purpose

    Gateway Donor vector containing CCL2, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, Matthew Meyerson, David Root

    Article

    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

    Insert

    CCL2 (CCL2 Human)
    

    Use

    Gateway entry vector

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti-PGK-RAP1A-WT-neo

    Plasmid

    #156173

    Purpose

    Lentiviral vector for expression of RAP1A-WT, with neomycin section

    Depositor

    Roland Friedel

    Article

    Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

    Insert

    RAP1A (RAP1A Human)
    

    Use

    Lentiviral

    Tags

    none

    Expression

    Mammalian

    Mutation

    wild type form

    Promoter

    human PGK

    Available Since

    July 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-T7-ABE8e-SpCas9-HiFi-P2A-EGFP (LM446)

    Plasmid

    #197506

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-HiFi(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    pCMV-T7-BPNLS-ABE8e-nSpCas9-HiFi-BPNLS-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8e mutations in TadA and nSpCas9-HiFi(D10A/R69…

    Promoter

    CMV and T7

    Available Since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pU6-pegRNA-LRRK2-G2019S-3a

    Plasmid

    #180432

    Purpose

    Transiently expressing a pegRNA to introduce LRRK2-G2019S mutation in human cells

    Depositor

    Frank Soldner

    Article

    Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

    Insert

    ncRNA targeting LRRK2-G2019S (LRRK2 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    LRRK2-G2019S

    Promoter

    U6

    Available Since

    Aug. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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