167,318 results

1. pCas9-sgAAVS1-1

   Plasmid

   #129726

   Purpose

   Expressing SpCas9 and sgAAVS1-1

   Depositor

   Elina Ikonen

   Article

   Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

   Insert

   SpCas9 and sgAAVS1-1
   

   Use

   CRISPR

   Tags

   P2A-Puro

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDZ2087

   Plasmid

   #92414

   Purpose

   Expresses His-tagged mutant (L56V/S135G/S219V) TEV protease in E. coli

   Depositor

   David Waugh

   Article

   Raran-Kurussi et al Methods Mol Biol. 2017;1586:221-230. doi

   Insert

   TEV protease
   

   Tags

   MBP (self cleaving)

   Expression

   Bacterial

   Mutation

   L56V/S135G/S219V

   Promoter

   tac

   Available Since

   July 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSUP-(2xCouRS-tRNAopt)

   Plasmid

   #197930

   Purpose

   Machinery plasmid expressing constitutive and inducible 7HCou synthetase and cognate amber suppressing tRNA. Incorporates (7-hydroxy-4-coumarin-yl) ethylglycine

   Depositor

   Kenneth Johnson

   Article

   Dangerfield et al J Biol Chem. 2020 Dec 11;295(50):17265-1

   Inserts

   CouRS
   tRNA opt
   

   Expression

   Bacterial

   Promoter

   glnS' and lambda(ts) and proK

   Available Since

   Aug. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. CMV500 A-CREB

   Plasmid

   #33371

   Depositor

   Charles Vinson

   Article

   Ahn et al Mol Cell Biol. 1998 Feb . 18(2):967-77.

   Insert

   CREB (Creb1 Mouse)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Dominant Negative (see notes)

   Promoter

   CMV

   Available Since

   Dec. 19, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLM1110_LEU2

   Plasmid

   #168460

   Purpose

   Big-IN Payload yeast assembly vector. Encodes LEU2 yeast selectable marker and components for bacterial copy number induction. Contains a mammalian transient (backbone) GFP-T2A-BSD selection cassette.

   Depositor

   Jef Boeke

   Article

   Brosh et al Proc Natl Acad Sci U S A. 2021 Mar 9;118

   Insert

   mRFP1
   

   Use

   Synthetic Biology; Yeast assembly vector for big-…

   Available Since

   June 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHR CD19-FcGamma CAR

   Plasmid

   #113014

   Purpose

   for production of lentivirus encoding the CD19 CAR-Pfcgamma

   Depositor

   Ron Vale

   Article

   Morrissey et al Elife. 2018 Jun 4;7. pii: 36688. doi: 10

   Insert

   CD19 CAR-P intracellular GFP (CD19 Human, Mouse, Synthetic)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   Aug. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-jAspSnFR3

   Plasmid

   #203488

   Purpose

   jAspSnFR3 in lentiviral expression vector

   Depositor

   Lucas Sullivan

   Article

   Davidsen et al eLife. 2024 Feb 23;12:RP90024. doi: 10.7

   Insert

   jAspSnFR3
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. TRE reporter backbone

   Plasmid

   #158678

   Purpose

   This plasmid serves as the backbone for the TRE reporters described in our paper. It's a promoterless-mStrawberry-pGK-BSD backbone to which we insert a pathway specific promoter before the mStrawberry

   Depositor

   Ravid Straussman

   Article

   Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   mStrawberry

   Expression

   Mammalian

   Available Since

   May 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCBh_NLS_hfCas13X(Cas13X_M17YY)_NLS-pA-U6-DR-BpiI-BpiI-DR-pSV40-EGFP-pA-pSV40-mCherry-pA

   Plasmid

   #190033

   Purpose

   vector for encoding a human codon-optimized High-fidelity Cas13X (hfCas13X) driven by CBh promoter, guide RNAs compatible with Cas13X driven by hU6, EGFP and mCherry driven by SV40 promoters

   Depositor

   Huawei Tong

   Article

   Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103

   Insert

   humanized hfCas13X
   

   Expression

   Mammalian

   Mutation

   Y672A,Y676A

   Promoter

   CBh, SV40, hU6

   Available Since

   Sept. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. PX856

    Plasmid

    #62888

    Purpose

    C-term SpCas9 piece of inducible transcriptional activator (dCas9(C)-FKBP-2xNLS-VP64)

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

    Insert

    SpCas9 (aa536-1368)
    

    Use

    CRISPR

    Tags

    FKBP12, NLS, NLS SV40, and VP64

    Expression

    Mammalian

    Mutation

    Asparagine 863 to Alanine (N863A)

    Promoter

    CBh

    Available Since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-Cyto-GCaMP3

    Plasmid

    #64853

    Purpose

    Cytosol-targeted version of the Ca2+ indicator GCaMP3.

    Depositor

    Jin Zhang

    Article

    Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

    Insert

    GCaMP3
    

    Tags

    Nuclear export signal (NES)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-Peredox-mCherry

    Plasmid

    #32383

    Depositor

    Gary Yellen

    Article

    Hung et al Cell Metab. 2011 Oct 5;14(4):545-54.

    Insert

    Peredox-mCherry
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pT3-EF1aH c-Met

    Plasmid

    #86498

    Purpose

    Mammalian expression of human c-Met

    Depositor

    Xin Chen

    Article

    Tward et al Proc Natl Acad Sci U S A. 2007 Sep 11;10

    Insert

    Met (MET Human)
    

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMRXIP GFP-Stx7

    Plasmid

    #45921

    Depositor

    Noboru Mizushima

    Article

    Itakura et al Cell. 2012 Dec 7;151(6):1256-69. doi: 10

    Insert

    syntaxin 7 (STX7 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Promoter

    CMV

    Available Since

    July 9, 2013

    Availability

    Academic Institutions and Nonprofits only

15. qTAG-C-mScarlet-EFS-Blast

    Plasmid

    #227260

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mScarlet-EFS-Blast cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. VPS13D^Halo

    Plasmid

    #174108

    Purpose

    The insert is a codon optimized DNA sequence encoding VPS13D with an internal Halo tag.

    Depositor

    Pietro De Camilli

    Article

    Guillen-Samander et al J Cell Biol. 2021 May 3;220(5). pii: 212

    Insert

    Codon optimized VPS13D
    

    Tags

    Halo

    Expression

    Mammalian

    Mutation

    With an internal Halo tag in aminoacid position 1…

    Promoter

    CMV

    Available Since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. lentiGuide-mCherry

    Plasmid

    #217005

    Purpose

    lentiviral plasmid for gRNA cloning with selectable mCherry expression

    Depositor

    Deepta Bhattacharya

    Article

    D'Souza et al PLoS One. 2022 Aug 24;17(8):e0261801. do

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHAGE-TO-dCas9-3XGFP

    Plasmid

    #64107

    Purpose

    Expression of Sp dCas9-3XGFP in mammalian cells

    Depositor

    Thoru Pederson

    Article

    Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

    Insert

    Sp dCas9
    

    Use

    Lentiviral

    Tags

    3XsfGFP

    Expression

    Mammalian

    Promoter

    CMV-TO

    Available Since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. human MMP-2 promoter

    Plasmid

    #53432

    Purpose

    promoter activity study

    Depositor

    Etty Benveniste

    Article

    Qin et al J Biol Chem. 1999 Oct 8;274(41):29130-7.

    Insert

    MMP2 promoter (MMP2 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Promoter

    MMP2

    Available Since

    June 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCEP4-191084-gp160

    Plasmid

    #123234

    Purpose

    Mammalian expression plasmid for Env from the 191084 B7-19 HIV-1 isolate

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (191084 B7-19) Env
    

    Tags

    CD5 leader peptide

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene

    Promoter

    CMV

    Available Since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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