We narrowed to 11,723 results for: Vars

1. pSpCas9n(BB)-2A-Puro (PX462) V2.0 Stx5DL-C2

   Plasmid

   #165086

   Purpose

   gRNA 2 of pair C for Stx5L-specific knockout

   Depositor

   Geert van den Bogaart

   Article

   Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

   Insert

   hSpCas9n-2A-Puro
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTwist-SARS-CoV-2 Δ18 B.1.351v3/wtRBD

   Plasmid

   #169468

   Purpose

   Encodes SARS-CoV-2 B.1.351v3/wtRBD Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

   Insert

   SARS-CoV-2 B.1.351v3/wtRBD Spike truncated to remove 18 amino acids (S )
   

   Expression

   Mammalian

   Available Since

   June 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTwist-SARS-CoV-2 Δ18 B.1.351v1/wtRBD

   Plasmid

   #169466

   Purpose

   Encodes SARS-CoV-2 B.1.351v1/wtRBD Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

   Insert

   SARS-CoV-2 B.1.351v1/wtRBD Spike truncated to remove 18 amino acids (S )
   

   Expression

   Mammalian

   Available Since

   June 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSico_U6-PGC1a1 sgRNA

   Plasmid

   #165425

   Purpose

   Expression of gRNA against human PGC-1a variant 1

   Depositor

   Antonio Amelio

   Article

   Musicant et al Cell Rep. 2021 Feb 23;34(8):108768. doi:

   Insert

   gRNA against PGC-1a variant 1 (PPARGC1A Synthetic, Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   April 9, 2021

   Availability

   Academic Institutions and Nonprofits only

5. pCMV ShadowY-AURKA-mTurq2

   Plasmid

   #157770

   Purpose

   Expression of AuroraA kinase biosensor under CMV promoter

   Depositor

   Marc Tramier

   Article

   Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

   Insert

   AURKA (AURKA Human)
   

   Tags

   mTurquoise2 and shadowY

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPGK-T7/2-CD44v2-10 (-cyt)

   Plasmid

   #137825

   Purpose

   expression of CD44 proteins in mammalian cells

   Depositor

   Ursula Gunthert

   Article

   Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

   Insert

   CD44v2-10 (-cyt) (CD44 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   without cytoplasmic region - N213S on the CD44 re…

   Promoter

   PGK

   Available Since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA 3.1 mitoAURKA Lys162Met

   Plasmid

   #157752

   Purpose

   Expression of kinase-dead AURKA K62M in mitochondria

   Depositor

   Marc Tramier

   Article

   Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

   Insert

   AURKA (AURKA Human)
   

   Expression

   Mammalian

   Mutation

   AURKA K162M is a kinase-dead version of AURKA

   Promoter

   CMV

   Available Since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP N1 AURKA 1-110

   Plasmid

   #157755

   Purpose

   Expression of AURKA Nter (aa1-110):GFP in mammalian cells

   Depositor

   Marc Tramier

   Article

   Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

   Insert

   AURKA (AURKA Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   The first 110 amino acids of AURKA were cloned

   Promoter

   CMV

   Available Since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP N1 AURKA 1-30

   Plasmid

   #157756

   Purpose

   Expression of AURKA Nter (aa1-30):GFP in mammalian cells

   Depositor

   Marc Tramier

   Article

   Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

   Insert

   AURKA (AURKA Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   The first 30 amino acids of AURKA were cloned

   Promoter

   CMV

   Available Since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA 3.1 AURKA deltaNter

    Plasmid

    #157749

    Purpose

    Expression of AURKA without Nter in mammalian cells

    Depositor

    Marc Tramier

    Article

    Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

    Insert

    AURKA (AURKA Human)
    

    Expression

    Mammalian

    Mutation

    the first 30AA of Aurora A are lacking

    Promoter

    CMV

    Available Since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCri-H6-TEV-GRAB

    Plasmid

    #128498

    Purpose

    Expresses GRAB (from V34 to N181) (Streptococcus pyogenes serotype M1) in bacterial cells. With N-t H6x tag + TEV.

    Depositor

    F Xavier Gomis-Ruth

    Article

    Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

    Insert

    GRAB (Streptococcus pyogenes serotype M1) (grab Streptococcus pyogenes serotype M1)
    

    Tags

    His tag (6x)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCri-H6-TEV-GRAB-STREP

    Plasmid

    #128499

    Purpose

    Expresses GRAB (from V34 to N181) in bacterial cells. With N-t H6x tag + TEV + protein + STREP(2x) tag.

    Depositor

    F Xavier Gomis-Ruth

    Article

    Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

    Insert

    GRAB (V34-N181) (grab Streptococcus pyogenes serotype M1)
    

    Tags

    His tag (6x) and Strep tag (2x)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUDP046

    Plasmid

    #107062

    Purpose

    Broad-host-range Cas9/gRNA co-expression plasmid with gRNA targetting KU80 from Ogataea parapolymorpha

    Depositor

    Jean-Marc Daran

    Article

    Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

    Insert

    HH-gRNA-HDV targetting OpKU80 inO. parapolymorpha
    

    Expression

    Yeast

    Promoter

    ScTDH3

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUC19-Q03JI6

    Plasmid

    #103123

    Purpose

    Cas9 coding gene template with optimized sequence for human codon usage

    Depositor

    Duhee Bang

    Article

    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

    Insert

    Q03JI6 (Cas9 coding gene from Campylobacter jejuni)
    

    Use

    CRISPR; Cloning vector

    Tags

    SV40NLS

    Mutation

    human codon-optimized

    Available Since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDONR223_SPANXN4_WT

    Plasmid

    #81998

    Purpose

    Gateway Donor vector containing SPANXN4, part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, William Hahn, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    SPANXN4 (SPANXN4 Human)
    

    Use

    Gateway entry vector

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDONR223_FAM200A_WT

    Plasmid

    #81878

    Purpose

    Gateway Donor vector containing FAM200A ,part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, William Hahn, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    FAM200A (FAM200A Human)
    

    Use

    Gateway entry vector

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDONR223_SPATA8_WT

    Plasmid

    #81866

    Purpose

    Gateway Donor vector containing SPATA8 , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, William Hahn, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    SPATA8 (SPATA8 Human)
    

    Use

    Gateway entry vector

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDONR223_SCEL_WT

    Plasmid

    #81870

    Purpose

    Gateway Donor vector containing SCEL , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, William Hahn, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    SCEL (SCEL Human)
    

    Use

    Gateway entry vector

    Mutation

    M124V; P201T

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDONR223_PRR23B_WT

    Plasmid

    #81871

    Purpose

    Gateway Donor vector containing PRR23B , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, William Hahn, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    PRR23B (PRR23B Human)
    

    Use

    Gateway entry vector

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDONR223_ZNF454_WT

    Plasmid

    #81864

    Purpose

    Gateway Donor vector containing ZNF454 , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, William Hahn, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    ZNF454 (ZNF454 Human)
    

    Use

    Gateway entry vector

    Mutation

    D166A

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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