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pGST1-yVps27-tUIM

Plasmid

#44428

Depositor

James Hurley

Article

Ren et al EMBO J. 2010 Mar 17;29(6):1045-54. Epub

Insert

yVps27 tandem UIM (245-330) (VPS27 Budding Yeast)

Tags

GST

Expression

Bacterial

Available Since

July 29, 2013

Availability

Academic Institutions and Nonprofits only
pGST1-yVps27-UIM2

Plasmid

#44427

Depositor

James Hurley

Article

Ren et al EMBO J. 2010 Mar 17;29(6):1045-54. Epub

Insert

Vps27-UIM2 (290-330) (VPS27 Budding Yeast)

Tags

GST

Expression

Bacterial

Available Since

July 29, 2013

Availability

Academic Institutions and Nonprofits only
pGST1-STAM1-UIM

Plasmid

#44425

Depositor

James Hurley

Article

Ren et al EMBO J. 2010 Mar 17;29(6):1045-54. Epub

Insert

STAM1-UIM (STAM Human)

Tags

GST

Expression

Bacterial

Available Since

July 29, 2013

Availability

Academic Institutions and Nonprofits only
pGST1-yGGA2-VHS

Plasmid

#44422

Depositor

James Hurley

Article

Ren et al EMBO J. 2010 Mar 17;29(6):1045-54. Epub

Insert

yGGA2 (1-169) (GGA2 Budding Yeast)

Tags

GST

Expression

Bacterial

Available Since

July 29, 2013

Availability

Academic Institutions and Nonprofits only
pGST1-yGGA1-VHS

Plasmid

#44421

Depositor

James Hurley

Article

Ren et al EMBO J. 2010 Mar 17;29(6):1045-54. Epub

Insert

yGGA1-VHS (GGA1 Budding Yeast)

Tags

GST

Expression

Bacterial

Available Since

July 29, 2013

Availability

Academic Institutions and Nonprofits only
pGST1-GGA2-VHS

Plasmid

#44419

Depositor

James Hurley

Article

Ren et al EMBO J. 2010 Mar 17;29(6):1045-54. Epub

Insert

GGA2-VHS (GGA2 Human)

Tags

GST

Expression

Bacterial

Mutation

Alanine 23 changed to Threonine

Available Since

July 26, 2013

Availability

Academic Institutions and Nonprofits only
Mito4x-LA-GCaMP6s

Plasmid

#243810

Purpose

Expresses a low affinity (LA) calcium sensor directed to the mitochondrial matrix

Depositor

Jaime de Juan-Sanz

Article

Amrapali Vishwanath et al Nat Metab. 2026 Feb 11. doi: 10.1038/s42

Insert

Mito4x-LAGCaMP6s

Expression

Mammalian

Mutation

D362I

Promoter

CMV

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-Camk2a(short)-mRFP1.Letm1[miR30-shRNA]-WPRE

Plasmid

#214404

Purpose

Expresses a mouse Letm1 specific microRNA in tandem with mRFP1

Depositor

Jaime de Juan-Sanz

Article

Amrapali Vishwanath et al Nat Metab. 2026 Feb 11. doi: 10.1038/s42

Insert

mRFP1 with Letm1 specific miRNA

Use

AAV

Expression

Mammalian

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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pLKO-U6-Letm1shRNA-hPGK-mTagBFP2

Plasmid

#215363

Purpose

Expresses a rat Letm1 specific shRNA and a BFP under a separate hPGK promoter

Depositor

Jaime de Juan-Sanz

Article

Amrapali Vishwanath et al Nat Metab. 2026 Feb 11. doi: 10.1038/s42

Insert

Letm1 shRNA Target sequence (Letm1 Rat)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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pLKO-U6-Letm1shRNA-hPGK-miRFPnano

Plasmid

#215365

Purpose

Expresses a rat Letm1 specific shRNA and miRFPnano under a separate hPGK promoter

Depositor

Jaime de Juan-Sanz

Article

Amrapali Vishwanath et al Nat Metab. 2026 Feb 11. doi: 10.1038/s42

Insert

Letm1 shRNA Target sequence (Letm1 Rat)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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CamKII-mito4x-GCaMP6f-IRES2-MCS

Plasmid

#212660

Purpose

Expresses under a CamKII promoter: mito4x-GCaMP6f and a multiple cloning site (MCS) after an IRES sequence

Depositor

Jaime de Juan-Sanz

Article

Amrapali Vishwanath et al Nat Metab. 2026 Feb 11. doi: 10.1038/s42

Inserts

mito-4x-GCaMP6f
Letm1 EF-hand mutant (Letm1 Rat)

Use

Lentiviral

Expression

Mammalian

Mutation

D676A, D680A

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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CamKII-mito4x- GCaMP6f-IRES2-rat-Letm1-ΔEFhand

Plasmid

#212662

Purpose

Expresses under a CamKII promoter: mito4x-GCaMP6f and rat-Letm1 with mutations in its EF-hand

Depositor

Jaime de Juan-Sanz

Article

Amrapali Vishwanath et al Nat Metab. 2026 Feb 11. doi: 10.1038/s42

Inserts

mito-4x-GCaMP6f
Letm1 EF-hand mutant (Letm1 Rat)

Use

Lentiviral

Expression

Mammalian

Mutation

D676A, D680A

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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CamKII-mito4x-GCaMP6f-Drosophila-Letm1

Plasmid

#212663

Purpose

Expresses under a CamKII promoter: mito4x-GCaMP6f and Drosophila-Letm1

Depositor

Jaime de Juan-Sanz

Article

Amrapali Vishwanath et al Nat Metab. 2026 Feb 11. doi: 10.1038/s42

Inserts

mito-4x-GCaMP6f
Letm1 (Letm1 Fly)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgCrls1-1#

Plasmid

#234765

Purpose

knockout Crls1

Depositor

Judy Lieberman

Article

Miao et al Immunity. 2023 Nov 14;56(11):2523-2541.e

Insert

Crls1 (Crls1 Mouse)

Use

Lentiviral

Available Since

March 27, 2025

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgCrls1-2#

Plasmid

#234766

Purpose

knockout Crls

Depositor

Judy Lieberman

Article

Miao et al Immunity. 2023 Nov 14;56(11):2523-2541.e

Insert

Crls1 (Crls1 Mouse)

Use

Lentiviral

Available Since

March 27, 2025

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgPlscr3-2#

Plasmid

#234772

Purpose

knockout Plscr3

Depositor

Judy Lieberman

Article

Miao et al Immunity. 2023 Nov 14;56(11):2523-2541.e

Insert

Plscr3 (Plscr3 Mouse)

Use

Lentiviral

Available Since

March 27, 2025

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgPlscr3-1#

Plasmid

#234767

Purpose

knockout Plscr3

Depositor

Judy Lieberman

Article

Miao et al Immunity. 2023 Nov 14;56(11):2523-2541.e

Insert

Plscr3 (Plscr3 Mouse)

Use

Lentiviral

Available Since

March 27, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-Tnnt2-Cre-U6-Lmna-sgRNA

Plasmid

#206178

Purpose

Expresses Cre recombinase specifically in cardiomyocytes and uses U6 promoter to express sgRNAs targeting murine Lmna exon 10

Depositor

Yuxuan Guo

Article

Sun et al JACC Basic Transl Sci. 2024 Aug 21;9(11)

Insert

Cre

Use

AAV

Promoter

cTnT

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-Loxp-CMV-Loxp-GFP-LA

Plasmid

#206199

Purpose

Expresses GFP-lamin-A only in non-myocyte when delivered into mice carrying a cardiomyocyte-specific Myh6-Cre transgene

Depositor

Yuxuan Guo

Article

Sun et al JACC Basic Transl Sci. 2024 Aug 21;9(11)

Insert

Lmna (LMNA Human)

Use

AAV

Tags

GFP

Promoter

CMV

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pSPL3-hRPH3A_c.444Tmut

Plasmid

#190477

Purpose

minigene assay

Depositor

Alfredo Brusco

Article

Pavinato et al Genet Med. 2023 Nov;25(11):100922. doi:

Insert

RPH3A (NM_014954.3) c.1853 [exon 20 and part of surrounding introns only] (RPH3A Human)

Expression

Mammalian

Mutation

c.444G>T

Promoter

SV40

Available Since

Sept. 16, 2022

Availability

Academic Institutions and Nonprofits only

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