We narrowed to 7,359 results for: GFP expression plasmids

1. SPACE (pRZ1813)

   Plasmid

   #140242

   Purpose

   CMV promoter expression plasmid for TadA*(V82G)-nCas9-pmCDA1(R187W)-UGI-UGI-P2A-EGFP

   Depositor

   Keith Joung

   Article

   Grunewald et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

   Insert

   SPACE
   

   Expression

   Mammalian

   Mutation

   V82G in TadA*, D10A in Cas9, R187W in pmCDA1

   Promoter

   CMV

   Available Since

   June 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. SPACE-NG (pRZ5150)

   Plasmid

   #140244

   Purpose

   CMV promoter expression plasmid for TadA*(V82G)-nCas9_NG-pmCDA1(R187W)-UGI-UGI-P2A-EGFP

   Depositor

   Keith Joung

   Article

   Grunewald et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

   Insert

   SPACE_NG
   

   Expression

   Mammalian

   Mutation

   V82G in TadA*, NG mutations in SpCas9, D10A in Sp…

   Promoter

   CMV

   Available Since

   June 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. SPACE-VRQR (pRZ5137)

   Plasmid

   #140245

   Purpose

   CMV promoter expression plasmid for TadA*(V82G)-nCas9_VRQR-pmCDA1(R187W)-UGI-UGI-P2A-EGFP

   Depositor

   Keith Joung

   Article

   Grunewald et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

   Insert

   SPACE_VRQR
   

   Expression

   Mammalian

   Mutation

   V82G in TadA*, VRQR mutations in SpCas9, D10A in …

   Promoter

   CMV

   Available Since

   June 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBABE-ProDOL

   Plasmid

   #206866

   Purpose

   Expression of ProDOL probe - LynAnchor-eGFP-SNAP-tag-HaloTag

   Depositor

   Dirk-Peter Herten

   Article

   Tashev et al Nat Methods. 2024 Sep;21(9):1708-1715. d

   Insert

   ProDOL
   

   Use

   Retroviral

   Tags

   HaloTag, His-Tag, SNAP-tag, and eGFP

   Expression

   Mammalian

   Mutation

   Contains Lyn amino acids 1-16

   Available Since

   Aug. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. XZ054 (PB)SFFV-LIDARsensor (hygR)

   Plasmid

   #233419

   Purpose

   PiggyBac donor plasmid with SFFV driven LIDAR reporter RNA (mCherry as marker, EGFP as output) for stable integration

   Depositor

   Xiaojing Gao

   Article

   Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

   Insert

   mCherry-SGc(stem-1(3xMS2))cSG-EGFP
   

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   SFFV

   Available Since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAID5.3-C

   Plasmid

   #145813

   Purpose

   An all-in-one bicistronic plasmid for controlling protein degradation by AID technology

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Pharmaceuticals (Basel). 2020 May 23;13(

   Type

   Empty backbone

   Tags

   mAID

   Expression

   Mammalian

   Available Since

   June 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAID5.1-C

   Plasmid

   #145809

   Purpose

   An all-in-one bicistronic plasmid for controlling protein degradation by AID technology

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Pharmaceuticals (Basel). 2020 May 23;13(

   Type

   Empty backbone

   Tags

   mAID

   Expression

   Mammalian

   Available Since

   June 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAID5.1-N

   Plasmid

   #145808

   Purpose

   An all-in-one bicistronic plasmid for controlling protein degradation by AID technology

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Pharmaceuticals (Basel). 2020 May 23;13(

   Type

   Empty backbone

   Tags

   mAID

   Expression

   Mammalian

   Available Since

   June 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAID5.3-N

   Plasmid

   #145812

   Purpose

   An all-in-one bicistronic plasmid for controlling protein degradation by AID technology

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Pharmaceuticals (Basel). 2020 May 23;13(

   Type

   Empty backbone

   Tags

   mAID

   Expression

   Mammalian

   Available Since

   June 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. mGFAP-Halo

    Plasmid

    #169474

    Purpose

    the host vector was obtained by substitution of the EGFP tag from a pEGFP-N3-mGFAP plasmid for a HaloTag using BamHI and NotI restriction sites

    Depositor

    Federico Herrera

    Article

    Letra-Vilela et al Experimental Results (2020), Volume 1, e

    Insert

    GFAP (Gfap Mouse)
    

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Changed R 236 to H

    Available Since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pWN10042

    Plasmid

    #89052

    Purpose

    GF-ori-FP plasmid for GFxFP assay

    Depositor

    Ervin Welker

    Article

    Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

    Inserts

    EGFP part 1
    EGFP part 2
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CAG and none

    Available Since

    April 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEG302-SunTag-FWAgRNA-4-22aa-TET1cd

    Plasmid

    #115344

    Purpose

    SunTag CRISPR cas9 system that targets the TET1 catalytic domain to the FWA promoter

    Depositor

    Steven Jacobsen

    Insert

    gRNA4_U6_NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Available Since

    March 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJP_1node

    Plasmid

    #230984

    Purpose

    pBAD promoter controls the expression of sfGFP. In the presence of VVD-AraC, blue light and L-arabinose can be used to activate mCitrine expression.

    Depositor

    Yolanda Schaerli

    Article

    Hollo et al Mol Syst Biol. 2025 Sep 29. doi: 10.1038

    Insert

    mCitrine
    

    Expression

    Bacterial

    Available Since

    Sept. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. LifeAct-14 (LA-14)

    Plasmid

    #158750

    Purpose

    Lentiviral plasmid for expression of shorter LifeAct variant (up till 14 amino acids) fused with carboxy terminus eGFP in mammalian cells.

    Depositor

    Minhajuddin Sirajuddin

    Article

    Kumari et al EMBO J. 2020 Jun 22:e104006. doi: 10.152

    Insert

    LifeAct-14
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Deletion mutation for last three amino acids from…

    Promoter

    CMV immediate early

    Available Since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. CaMK2rep-S603A

    Plasmid

    #239623

    Purpose

    Inactive CaMKII activity reporter. The plasmid contains sGFP2 fluorescent protein, 3xmyc tags and double mutated phospho-site 3 from rat Synapsin-1a.

    Depositor

    F Javier Díez Guerra

    Article

    Martinez-Blanco et al Anal Chem. 2025 Sep 23;97(37):20275-2029

    Insert

    SpotNES-sGFP2-SynP3-3xmyc (Syn1 Synthetic, Rat)
    

    Tags

    Spot, myc, and sGFP2

    Expression

    Mammalian

    Mutation

    changed Serine 603 to Alanine

    Promoter

    CMV

    Available Since

    July 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV_ACTB CRISPIE

    Plasmid

    #172853

    Purpose

    Actb sgRNA & DRS-2 sgRNA expression under U6. CRISPIE donor mEGFP phase (0-0), excised by DRS-1 or DRS-2 (with SpCas9). Also expresses mRuby3 under the CBA promotor. See Zhong et al, eLife 2021.

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Inserts

    Actb intron 1 sgRNA
    DRS-2 sgRNA
    CRISPIE designer exon (phase 0-0) encoding mEGFP
    mRuby3
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. scFv-GCN4-DNMT3a(R887E)-DNMT3l

    Plasmid

    #154141

    Purpose

    Expresses a higher specificity variant of scFv-GCN4-DNMT3a-DNMT3l, contains R887E mutation in DNMT3a. To be used with the dCas9-SunTag system for targeted DNA methylation.

    Depositor

    Albert Jeltsch

    Article

    Hofacker et al Int J Mol Sci. 2020 Jan 13;21(2). pii: i

    Insert

    scFv-GCN4, DNMT3a (catalytic domain), DNMT3l (C-terminal part), sfGFP
    

    Tags

    HA and sfGFP

    Expression

    Mammalian

    Mutation

    R887E

    Promoter

    SFFV

    Available Since

    Oct. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. miniABEmax (pRZ900)

    Plasmid

    #131311

    Purpose

    CMV promoter expression plasmid for bpNLS-TadA7.10-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (ABEmax with truncation of WT TadA domain).

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

    Insert

    bpNLS-TadA7.10-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCBh-T7-SpCas9-P2A-mTagBFP2(RAS3575)

    Plasmid

    #223111

    Purpose

    pCBh Human expression plasmid for SpCas9 with with C-term BPNLS-P2A-mTagBFP2

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized nuclease SpCas9-P2A-mTagBFP2
    

    Use

    CRISPR

    Tags

    BPNLS-3xFLAG-P2A-mTagBFP2

    Expression

    Mammalian

    Promoter

    CMV and T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. SECURE miniABEmax-V82G (pJUL1828)

    Plasmid

    #131313

    Purpose

    CMV promoter expression plasmid for bpNLS-TadA7.10(V82G)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (miniABEmax with V82G mutation).

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

    Insert

    bpNLS-TadA7.10(V82G)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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