We narrowed to 12,024 results for: pcdna

1. pcDNA5-FRT/TO-3*Flag-APEX2 C-term SRSF7

   Plasmid

   #187582

   Purpose

   Express FLAG epitope and APEX2-tagged SRSF7 fusion protein in mammalian cells

   Depositor

   Benjamin Blencowe

   Article

   Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

   Insert

   SRSF7 (SRSF7 Human)
   

   Use

   Tags

   APEX2-FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5/FRT/TO_H2B-HaloTag10-T2A-EGFP

   Plasmid

   #175521

   Purpose

   Nuclear expression of HaloTag10 in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   H2B-HaloTag10
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   HaloTag10 = HaloTag7-Q165W

   Promoter

   Available Since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDNA5/FRT/TO_TOM20-dHaloTag7_T2A_CalR- HaloTag7-KDEL

   Plasmid

   #187079

   Purpose

   Co-expression of dHaloTag7 at the mitochondria surface and HaloTag7 in endoplasmic reticulum

   Depositor

   Kai Johnsson

   Article

   Kompa et al J Am Chem Soc. 2023 Feb 8;145(5):3075-30

   Insert

   TOM20-dHaloTag7_T2A_CalR- HaloTag7-KDEL
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   dHaloTag7 = HaloTag7-D106A

   Promoter

   Available Since

   Aug. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. Carl-CeNL(Ca2+)_110μ-KDEL-pcDNA3

   Plasmid

   #111925

   Purpose

   Cyan color luminescent Ca2+ indicator targeted to endoplasmic reticulum. Calreticulin and a KDEL signal for ER retention located at the N-terminus and C-terminus of CeNL(Ca2+)_110μ

   Depositor

   Takeharu Nagai

   Article

   Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

   Insert

   CeNL(Ca2+)_110μ
   

   Use

   Tags

   Calreticulin and KDEL

   Expression

   Mammalian

   Mutation

   E31D, F92W, E104D. D133E at CaM

   Promoter

   CMV

   Available Since

   June 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA5/FRT/TO_H2B-HaloTag11-T2A-EGFP

   Plasmid

   #175522

   Purpose

   Nuclear expression of HaloTag11 in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   H2B-HaloTag11
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   HaloTag11 = HaloTag7-M175W

   Promoter

   Available Since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA3.1(+) miniTurboID-6xGGSG linker-FKBPF36V-2xHA

   Plasmid

   #200641

   Purpose

   Expresses miniTurboID-FKBPF36V-2xHA in mammalian cells.

   Depositor

   Justin English, Fleur Ferguson

   Article

   Tao et al Nat Commun. 2023 Dec 4;14(1):8016. doi:

   Insert

   miniTurboID-6xGGSG linker-FKBPF36V-2xHA
   

   Use

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available Since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-dCas9-Dnmt3a-Dnmt3l-P2A-eGFP

   Plasmid

   #128424

   Purpose

   Human Codon Optimized dCas9-DNMT3A3L

   Depositor

   Bradley Bernstein

   Article

   Tarjan et al Nat Commun. 2019 Sep 18;10(1):4258. doi:

   Insert

   dCas9m4-sv40NLS-DNMT3A-3L-sv40NLS-P2A-eGFP
   

   Use

   CRISPR and Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   T7 Promoter

   Available Since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)_IRES_eRF1(E55D)-HA

   Plasmid

   #182653

   Purpose

   Encodes codon-optimized AF mutant of M. mazei pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF),tRNACUAPyl & eRF1E55D used for amber codon suppression in mammalian cells

   Depositor

   Ivana Nikić-Spiegel

   Article

   Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

   Inserts

   codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
   PylT
   mutant eukaryotic release factor 1
   

   Use

   Tags

   HA tag and nuclear export signal (NES)

   Expression

   Mammalian

   Mutation

   E55D and Y306A/Y384F (AF) double mutant of Methan…

   Promoter

   CMV and U6

   Available Since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA 3.1(Hygro) myc-hPolQ-Flag

   Plasmid

   #73132

   Purpose

   To express in mammalian cells human PolQ

   Depositor

   Agnel Sfeir

   Article

   Mateos-Gomez et al Nature. 2015 Feb 12;518(7538):254-7. doi

   Insert

   Polymerase theta (POLQ Human)
   

   Use

   Tags

   Flag and myc

   Expression

   Mammalian

   Mutation

   Promoter

   cmv promoter

   Available Since

   March 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA-puro-TagBFP-β-ACTIN-3′UTR-24xMS2

    Plasmid

    #205161

    Purpose

    express β-ACTIN-3′UTR-24xMS2

    Depositor

    Weirui Ma

    Article

    Hu et al Elife. 2023 Mar 3;12:e82178. doi: 10.755

    Insert

    β-ACTIN-3′UTR-24xMS2
    

    Use

    Tags

    tagBFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3 Ras-LOCKR-S R89L (negative control)

    Plasmid

    #216497

    Purpose

    FRET-based reporter for Ras activity, untargeted, negative control

    Depositor

    David Baker

    Article

    Zhang et al Nat Biotechnol. 2024 Jan 25. doi: 10.103

    Insert

    Ras-LOCKR-S R89L negative control
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-EF1A-mNeonGreen2x-SREBP1-P2A-Puro

    Plasmid

    #228401

    Purpose

    Encodes a N-terminal truncated version of SREBP1 lacking part of the transcriptional activation domain and fused to a tandem of mNeongreen to visualize the import of SREBP1

    Depositor

    Dirk Daelemans

    Article

    Vercruysse et al Commun Biol. 2024 Nov 23;7(1):1560. doi:

    Insert

    SREBP1 (SREBF1 Human)
    

    Use

    Tags

    mNeonGreen2x

    Expression

    Mammalian

    Mutation

    deleted N terminal activation domain (amino acids…

    Promoter

    EF1a

    Available Since

    Jan. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1-HaloAKAR2.1-NES-T2A-EGFP-CAAX

    Plasmid

    #216491

    Purpose

    Expression of chemigenetic PKA biosensor (medium basal brightness, high dynamic range) and PM targeted EGFP in mammalian cells

    Depositor

    Jin Zhang

    Article

    Frei et al Nat Biotechnol. 2025 Apr 21. doi: 10.103

    Insert

    HaloAKAR2.1
    

    Use

    Tags

    NES, T2A-EGFP-CAAX

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA5-FRT/TO-3*Flag-APEX2 C-term 3NLS

    Plasmid

    #187588

    Purpose

    Express FLAG epitope and APEX2-tagged 3xNLS fusion protein in mammalian cells

    Depositor

    Benjamin Blencowe

    Article

    Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

    Insert

    NLS
    

    Use

    Tags

    APEX2-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA5-FRT/TO-3*Flag-APEX2 C-term PML

    Plasmid

    #187583

    Purpose

    Express FLAG epitope and APEX2-tagged PML fusion protein in mammalian cells

    Depositor

    Benjamin Blencowe

    Article

    Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

    Insert

    PML (PML Human)
    

    Use

    Tags

    APEX2-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3 mHIF-1α MYC (P402A/P577A/N813A)

    Plasmid

    #44028

    Depositor

    Celeste Simon

    Article

    Hu et al Mol Biol Cell. 2007 Nov;18(11):4528-42.

    Insert

    mHIF-1α (P402A/P577A/N813A) (Hif1a Mouse)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    P402A, P577A, N813A

    Promoter

    CMV

    Available Since

    March 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1(+)_4x(U6 tRNA M15)_CMV NESPylRS(AF)

    Plasmid

    #182652

    Purpose

    Encodes codon-optimized AF mutant of M. mazei pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF) & 4x M15 tRNACUA used for amber codon suppression in mammalian cells

    Depositor

    Ivana Nikić-Spiegel

    Article

    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

    Inserts

    codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
    4xU6-tRNAM15
    

    Use

    Tags

    nuclear export signal (NES)

    Expression

    Mammalian

    Mutation

    Y306A/Y384F (AF) double mutant of Methanosarcina …

    Promoter

    CMV and U6

    Available Since

    May 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3_SP-GLP1R-V2R-NTEV-TCS-GV-2xHA

    Plasmid

    #194381

    Purpose

    Split TEV assays

    Depositor

    Michael Wehr

    Article

    Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

    Insert

    SP-GLP1R-V2R-NTEV-TCS-GV-2xHA (GLP1R Synthetic, Human)
    

    Use

    Tags

    2xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA5-FRT/TO-3*Flag-APEX2 N-term PSPC1

    Plasmid

    #187579

    Purpose

    Express FLAG epitope and APEX2-tagged PSPC1 fusion protein in mammalian cells

    Depositor

    Benjamin Blencowe

    Article

    Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

    Insert

    PSPC1 (PSPC1 Human)
    

    Use

    Tags

    APEX2-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA5-FRT/TO-3*Flag-APEX2 N-term NONO

    Plasmid

    #187580

    Purpose

    Express FLAG epitope and APEX2-tagged NONO fusion protein in mammalian cells

    Depositor

    Benjamin Blencowe

    Article

    Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

    Insert

    NONO (NONO Human)
    

    Use

    Tags

    APEX2-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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