Purpose
Expresses both fragments of T7 RNAP split at position 179. Both fragments are driven by PBAD. The C-terminal fragment has the point mutation N748D. Contains a constitutive araC ORF
Inserts
Residues 1-179 of split T7 RNAP
Residues 180-880 of split T7 RNAP
Use
Synthetic Biology
Tags
Expression
Bacterial
Mutation
Residues 1-180 of split T7 RNAP and Residues 180-…
Available since
Oct. 2, 2015
Availability
Academic Institutions and Nonprofits only