We narrowed to 16,082 results for: NOL

1. pCMV-hA3C-BE3

   Plasmid

   #113412

   Purpose

   Expresses hA3C-BE3 in mammalian cells

   Depositor

   Jia Chen

   Article

   Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

   Insert

   hA3C-BE3 (APOBEC3C S. pyogenes and Bacteriophage PBS2, Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONR221_SLC39A8

   Plasmid

   #132177

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Article

   Polesel et al Biometals. 2022 Dec 1. doi: 10.1007/s105

   Insert

   SLC39A8 (SLC39A8 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 25, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pYPQ265F2

   Plasmid

   #164722

   Purpose

   Gateway entry clone (attL1 & attR5) A3A/Y130F-nCas9-NG-CBE_V01 for C-T base editing

   Depositor

   Yiping Qi

   Article

   Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

   Insert

   A3A(Y130F)-zCas9(D10A)NG-UGI
   

   Use

   CRISPR; Gateway compatible a3a/y130f-ncas9-ng-cbe…

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLentiRNACRISPR_002 - hU6-DR_BsmBI-EFS-PguCas13b-NES-2A-Puro-WPRE

   Plasmid

   #138144

   Purpose

   Expresses PguCas13b in mammalian cells, localized in the cytoplasm. For cloning of guide RNAs compatible with PguCas13b. Contains a 3' direct repeat. Clone using BsmBI. F overhang cacc. R overhang caac

   Depositor

   Neville Sanjana

   Article

   Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

   Insert

   PguCas13b
   

   Use

   Lentiviral

   Tags

   NES

   Expression

   Mutation

   Promoter

   Available since

   Feb. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV_RBMX-eA3A-UdgX-nCas9

   Plasmid

   #163546

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   RBMX-eA3A-UdgX-nCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)

   Promoter

   Available since

   Dec. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV_eA3A T31A-dCas9

   Plasmid

   #163537

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   eA3A T31A-dCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   dCas9 (D10A, H840A)eA3A T31A

   Promoter

   Available since

   Dec. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR221_SLC39A10

   Plasmid

   #132275

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Article

   Polesel et al Biometals. 2022 Dec 1. doi: 10.1007/s105

   Insert

   SLC39A10 (SLC39A10 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 14, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pX458_Ruby

   Plasmid

   #110164

   Purpose

   Cas9 from S. pyogenes with Ruby2, and cloning backbone for sgRNA

   Depositor

   Philip Hublitz

   Article

   Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

   Insert

   hSpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and Ruby2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-BE3-FNLS(RA)

   Plasmid

   #112671

   Purpose

   CMV expression vector for BE3-FNLS construct (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   BE3-FNLS
   

   Use

   Tags

   3x FLAG

   Expression

   Mammalian

   Mutation

   NLS sequence at the N-terminus and D10A

   Promoter

   CMV

   Available since

   July 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGEX CrkL-SH2

    Plasmid

    #46419

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Crkl (CRKL Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 9-108

    Promoter

    Tac

    Available since

    July 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. SARS_COV2_ORF6_HA_BASU

    Plasmid

    #153984

    Purpose

    Express Orf6 gene along with HA and BASU

    Depositor

    Paul Khavari

    Article

    Meyers et al PLoS Pathog. 2021 Oct 1;17(10):e1009412.

    Insert

    orf6 (ORF6 Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV_eA3A-NG-nCas9

    Plasmid

    #163536

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    eA3A-NG-nCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…

    Promoter

    Available since

    Dec. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDR366 RNF2

    Plasmid

    #47509

    Purpose

    human gRNA expression vector targeting RNF2

    Depositor

    Keith Joung

    Article

    Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

    Insert

    gRNA-RNF2 (RNF2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. SARS_COV2_ORF8_HA_BASU

    Plasmid

    #153982

    Purpose

    Express Orf8 gene along with HA and BASU

    Depositor

    Paul Khavari

    Article

    Meyers et al PLoS Pathog. 2021 Oct 1;17(10):e1009412.

    Insert

    orf8 (ORF8 Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. SARS_COV2_nsp14_HA_BASU

    Plasmid

    #153993

    Purpose

    Express NSP14 gene along with HA and BASU

    Depositor

    Paul Khavari

    Article

    Meyers et al PLoS Pathog. 2021 Oct 1;17(10):e1009412.

    Insert

    NSP14 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. non-targeting control gRNA (BRDN0001146004)

    Plasmid

    #80174

    Purpose

    3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    Non-targeting control gRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Aug. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMonAID_dCas9-PmCDA_Hyg_ALS

    Plasmid

    #91692

    Purpose

    Monocot Target-AID vector expressing rice-optimized dCas9-PmCDA1 with sgRNA targeting OsAls

    Depositor

    Akihiko Kondo

    Article

    Shimatani et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    SpCas9
    

    Use

    Tags

    PmCDA1

    Expression

    Plant

    Mutation

    D10A and H840A for dead Cas9

    Promoter

    2x35S

    Available since

    June 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pYB-Dual(mNG(yeast-opt))

    Plasmid

    #216727

    Purpose

    Dual-species protein expression vector for high-level and inducible protein expression of mNeonGreen (codon optimized for yeast) in both yeast and bacteria from the same construct.

    Depositor

    Emil Jensen

    Article

    Waneskog et al Biotechnol Prog. 2024 May 17:e3482. doi:

    Insert

    mNeonGreen
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    pGAL1(yeast) and pT7-RNAP(bacteria)

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-ACBEmax

    Plasmid

    #139106

    Purpose

    Mammalian ACBEmax expression vector (pSI-632)

    Depositor

    Nozomu Yachie

    Article

    Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    Bi_partite_NLS-TadA*-XTEN_linker-TadA-XTEN_linker-SpCas9(D10A)-SH3_linker-rAPOBEC1-2xUGI-SV40NLS
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    June 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGW011b Dual AAV vector pAAV-EFS-dCAS9-spA

    Plasmid

    #192160

    Purpose

    Dual AAV vector AAV-dCas9

    Depositor

    Sidi Chen

    Article

    Wang et al Nat Immunol. 2019 Nov;20(11):1494-1505.

    Insert

    Dead Cas9
    

    Use

    AAV

    Tags

    Expression

    Mutation

    NA

    Promoter

    Available since

    Oct. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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21. Agarabi CHO Light Chain in pJ201

    Plasmid

    #111566

    Purpose

    Cloning vector containing CDS for the murine light chain of the model chimeric mouse-human IgG1 expressed by the Agarabi CHO cell line

    Depositor

    Cyrus Agarabi

    Article

    Velugula-Yellela et al Biotechnol Prog. 2018 Jan;34(1):262-270.

    Insert

    Murine light chain
    

    Use

    Synthetic Biology; Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    None

    Available since

    June 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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22. pVC299 VEGF Site#2

    Plasmid

    #47506

    Purpose

    human gRNA expression vector targeting VEGF

    Depositor

    Keith Joung

    Article

    Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

    Insert

    gRNA-VEGF site 2 (VEGFA Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. ABE8.17-d

    Plasmid

    #136299

    Purpose

    expresses ABE8.17-d in mammalian cells

    Depositor

    Nicole Gaudelli

    Article

    Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

    Insert

    ABE8.20-d
    

    Use

    Tags

    C-terminal BPNLS

    Expression

    Mammalian

    Mutation

    D10A in S. pyogenes Cas9, TadA mutations describe…

    Promoter

    Available since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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24. BMR1_01g02031-bio-His

    Plasmid

    #108116

    Purpose

    [Edit] Expresses enzymatically monobiotinylated full-length BMR1_01g02031 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag

    Depositor

    Gavin Wright

    Article

    Elton et al Int J Parasitol. 2019 Feb;49(2):115-125.

    Insert

    BMR1_01g02031
    

    Use

    Tags

    ratCD4d3+4,biotinylation sequence, 6xHis

    Expression

    Mammalian

    Mutation

    all potential N-linked glycosylation sites (N-X-S…

    Promoter

    CMV

    Available since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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25. A*03:02:01

    Plasmid

    #203294

    Purpose

    Overexpress HLA allele

    Depositor

    Catherine Wu, Derin Keskin

    Article

    Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

    Insert

    A*03:02:01 (HLA-A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pUCmini-iCAP-PHP.B3

    Plasmid

    #103004

    Purpose

    non-standard AAV2 rep-AAV-PHP.B3 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

    Depositor

    Viviana Gradinaru

    Article

    Deverman et al Nat Biotechnol. 2016 Feb;34(2):204-9. do

    Insert

    Synthetic construct isolate AAV-PHP.B3 VP1 gene
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    p41

    Available since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pUCmini-iCAP-PHP.B2

    Plasmid

    #103003

    Purpose

    non-standard AAV2 rep-AAV-PHP.B2 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

    Depositor

    Viviana Gradinaru

    Article

    Deverman et al Nat Biotechnol. 2016 Feb;34(2):204-9. do

    Insert

    Synthetic construct isolate AAV-PHP.B2 VP1 gene
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    p41

    Available since

    Dec. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. B*58:02:01

    Plasmid

    #203262

    Purpose

    Overexpress HLA allele

    Depositor

    Catherine Wu, Derin Keskin

    Article

    Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

    Insert

    B*58:02:01 (HLA-B Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. A*66:01:01

    Plasmid

    #203291

    Purpose

    Overexpress HLA allele

    Depositor

    Catherine Wu, Derin Keskin

    Article

    Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

    Insert

    A*66:01:01 (HLA-A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET28b-BE3∆UGI

    Plasmid

    #96943

    Purpose

    The plasmid encoding the His6-rAPOBEC1-XTEN-nCas9 protein (BE3∆UGI) was generated by site-directed mutagenesis using pET28b-BE1 (Addgene plasmid #73018).

    Depositor

    Daesik Kim

    Article

    Kim et al Nat Biotechnol. 2017 May;35(5):475-480.

    Insert

    BE3∆UGI
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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