We narrowed to 10,802 results for: T7

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pBz110_CsoS2_CTD

Plasmid

#140844

Purpose

His-tagged CsoS2 C-terminal domain (CTD), pET-14b backbone

Depositor

David Savage

Article

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

CsoS2 C-terminal domain (CTD)

Tags

Hexahistidine tag

Expression

Bacterial

Mutation

deleted amino acids 1-594

Promoter

T7

Available Since

May 8, 2020

Availability

Academic Institutions and Nonprofits only

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pHis8-4b-McDAH

Plasmid

#140129

Purpose

Expresses McDAH with N-terminal 8xHis-tag in BL21 E. coli

Depositor

Jing-Ke Weng

Article

Kim et al Nat Commun. 2020 Apr 20;11(1):1867. doi:

Insert

Dechloroacutumine halogenase from Menispermum canadense

Tags

8x-His, TEV protease site (ENLYFQ)

Expression

Bacterial

Promoter

T7

Available Since

April 21, 2020

Availability

Academic Institutions and Nonprofits only

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pHBDuet139

Plasmid

#121913

Purpose

Charge-Introduced NpuDnaB mini-intein

Depositor

Hideo Iwai

Article

Beyer et al FEBS J. 2019 Oct 30. doi: 10.1111/febs.1

Insert

CI-NpuDnaBΔ290 intein

Tags

GB1 and His6-GB1

Expression

Bacterial

Mutation

Deletion of 290-residue endonuclease domain, I53K…

Promoter

T7

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pHBDuet140

Plasmid

#121914

Purpose

Orthogonal NpuDnaB mini-intein intein

Depositor

Hideo Iwai

Article

Beyer et al FEBS J. 2019 Oct 30. doi: 10.1111/febs.1

Insert

Oth-NpuDnaBΔ290 intein

Tags

GB1 and His6-GB1

Expression

Bacterial

Mutation

Deletion of 290-residue endonuclease domain, I53K…

Promoter

T7

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pNIC28-BSA4-S15

Plasmid

#127821

Purpose

Production of recombinant human ribosomal protein S15

Depositor

Ivanhoe Leung

Article

Correddu et al Sci Rep. 2019 Jun 20;9(1):8884. doi: 10.

Insert

RPS15 (RPS15 Human)

Expression

Bacterial

Promoter

T7

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pNH-TrxT-G-S10

Plasmid

#127829

Purpose

Production of recombinant human ribosomal protein S10

Depositor

Ivanhoe Leung

Article

Correddu et al Sci Rep. 2019 Jun 20;9(1):8884. doi: 10.

Insert

RPS10 (RPS10 Human)

Tags

His6 - Thioredoxin - TEV

Expression

Bacterial

Promoter

T7

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pACYCDuet_W22HDHFR_WTTS

Plasmid

#91241

Purpose

bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

Depositor

Kimberly Reynolds

Article

Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

Inserts

folA
thyA

Tags

6xHis

Expression

Bacterial

Mutation

W22H

Promoter

T7

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pACYCDuet_F31VDHFR_WTTS

Plasmid

#91227

Purpose

bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

Depositor

Kimberly Reynolds

Article

Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

Inserts

folA
thyA

Tags

6xHis

Expression

Bacterial

Mutation

F31V

Promoter

T7

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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kank1a_pJet1.2

Plasmid

#121978

Purpose

for generating kank1a riboprobes for WISH in zebrafish

Depositor

GuangJun Zhang

Article

Hensley et al Sci Rep. 2016 Jun 13;6:27816. doi: 10.10

Insert

kank1a (si:dkey-146l2.1 Zebrafish)

Use

Cloning vector

Promoter

T7

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only

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MtMscL delta49

Plasmid

#118342

Purpose

M. tuberculosis MscL (delta49) in pET19b

Depositor

Douglas Rees

Article

Herrera et al Sci Rep. 2018 Oct 1;8(1):14566. doi: 10.

Insert

MscL (mscL Mycobacterium tuberculosis)

Tags

6x His Tag and Thrombin Cleavage site

Expression

Bacterial

Mutation

Changed Glutamic Acid (Glu) 102 to Stop Codon

Promoter

T7 Promoter

Available Since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pET51b(+)-LUCIDRS-MTX

Plasmid

#113925

Purpose

Plasmid encoding the bacterial expression of the red-shifted LUCID semi-synthetic BRET biosensor for methotrexate

Depositor

Kai Johnsson

Article

Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

Insert

LUCID_RS-MTX

Use

Luciferase

Expression

Bacterial

Promoter

T7

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a-clbH-C-A2-PCP

Plasmid

#114157

Purpose

Expresses ClbH-C-A2-PCP

Depositor

Emily Balskus

Article

Zha et al Nat Chem Biol. 2017 Oct;13(10):1063-1065

Insert

clbH

Tags

6xHis-thrombin-T7 tag

Expression

Bacterial

Mutation

Only contains the C-A2-PCP domains of ClbH

Available Since

Aug. 31, 2018

Availability

Academic Institutions and Nonprofits only

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mTalin2_R7R8

Plasmid

#111041

Purpose

Plasmid expresses the talin2 rod domains R7 and R8

Depositor

Ben Goult

Article

Bouchet et al Elife. 2016 Jul 13;5. doi: 10.7554/eLife

Insert

TLN2 (Tln2 Mouse)

Tags

His Tag

Expression

Bacterial

Promoter

T7

Available Since

July 3, 2018

Availability

Academic Institutions and Nonprofits only

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mTalin2_R7

Plasmid

#111039

Purpose

Plasmid expresses the talin2 rod domain R7 a 5 helix bundle.

Depositor

Ben Goult

Article

Bouchet et al Elife. 2016 Jul 13;5. doi: 10.7554/eLife

Insert

TLN2 (Tln2 Mouse)

Tags

His Tag

Expression

Bacterial

Promoter

T7

Available Since

June 15, 2018

Availability

Academic Institutions and Nonprofits only

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pET28b-NmCas9-Flag D16A

Plasmid

#109031

Purpose

D16A mutant of Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expression

Depositor

Yan Zhang

Article

Rousseau et al Mol Cell. 2018 Mar 1;69(5):906-914.e4. d

Insert

CRISPR-associated endonuclease Cas9

Tags

Flag Tag

Expression

Bacterial

Promoter

T7

Available Since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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pET15b+R2(L171C)

Plasmid

#108239

Purpose

Expresses PdR-PCNA2(L171C) fusion protein in E. coli

Depositor

Teruyuki Nagamune

Article

Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

Insert

PdR-PCNA2(L171C) fusion protein

Tags

His6 tag

Expression

Bacterial

Mutation

PCNA2,L171C

Promoter

T7 promoter

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pEHIShqprtR161Q

Plasmid

#92413

Purpose

hQPRTase harboring R161Q mutation

Depositor

Huanting Liu, James Naismith

Article

Liu et al J Mol Biol. 2007 Oct 26;373(3):755-63. E

Insert

hQPRTase (QPRT Human)

Tags

TEV protease cleavable 6xHis

Expression

Bacterial

Mutation

R161Q

Promoter

T7

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pK RagA(R37P)-RagC

Plasmid

#99706

Purpose

Bacterial expression of RagA(R37P)-RagC

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Inserts

RagA (RRAGA Human)
RagC (RRAGC Human)

Tags

His tag, Poly Arg, and SUMO

Expression

Bacterial

Mutation

R37P

Promoter

T7

Available Since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pK RagA-RagC(L91P)

Plasmid

#99707

Purpose

Bacterial expression of RagA-RagC(L91P)

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Inserts

RagA (RRAGA Human)
RagC (RRAGC Human)

Tags

His tag, Poly Arg, and SUMO

Expression

Bacterial

Mutation

L91P

Promoter

T7

Available Since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pK RagA-RagC(K84T)

Plasmid

#99708

Purpose

Bacterial expression of RagA-RagC(K84T)

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Inserts

RagA (RRAGA Human)
RagC (RRAGC Human)

Tags

His tag, Poly Arg, and SUMO

Expression

Bacterial

Mutation

K84T

Promoter

T7

Available Since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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