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Plasmid#89619Purpose[Edit] Expresses recombinant 6HIS tagged T. cruzi mitochondrial tryparedoxin peroxidase protein in E. coli upon IPTG inductionDepositorInsertMitocondrial Tryparedoxin peroxidase
Tags6HIS and T7 tag (gene 10 leader)ExpressionBacterialAvailable SinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-CMV.Pro
Plasmid#79486PurposeMultiSite Gateway entry clones, first fragment, (attL1, attR5), CMV promoterDepositorInsertCMV Promoter
UseMultisite gateway entry clonePromoterCMV PromoterAvailable SinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
GST-PC-1-CTS
Plasmid#86067PurposeA bacterial expression plasmid encoding polycystin-1 ciliary targeting signal with N-terminus GST-tag.DepositorInsertPolycystin-1 (PKD1 Human)
TagsGSTExpressionBacterialMutationcontains the region from 4297-4303PromotertacAvailable SinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET21a - TDR
Plasmid#89627PurposeExpresses recombinant 6HIS tagged T. cruzi thiol dependent reductase in E. coli upon IPTG inductionDepositorInsertthiol dependent reductase
Tags6HIS and T7 tag (gene 10 leader)ExpressionBacterialAvailable SinceApril 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET21a - NFDD
Plasmid#89624PurposeExpresses recombinant 6HIS tagged T. cruzi NAD-FAD–dependent dehydrogenase in E. coli upon IPTG inductionDepositorInsertNAD/FAD dependent dehydrogenase
Tags6HIS and T7 tag (gene 10 leader)ExpressionBacterialAvailable SinceApril 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDA186
Plasmid#74250PurposeIntermediate cloning vector, with MCS2 containing inducible unstable peptide driven by pGPD1 (UbiY 2xNLSs SZ3).DepositorInsertInducible peptide of the dPSTR
ExpressionBacterial and YeastPromoterpGPD1Available SinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDA171
Plasmid#74251PurposeIntermediate cloning vector, with MCS2 containing inducible stable peptide driven by pSTL1 (Venus 2xNLSs SZ1).DepositorInsertInducible peptide of the dPSTR
TagsVenusExpressionBacterial and YeastPromoterpSTL1Available SinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-2-Scmh1-3'UTR
Plasmid#61785PurposeLuciferase reporter containing the 3'UTR of mouse Scmh1DepositorInsertmouse Scmh1 3'UTR
UseLuciferaseAvailable SinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-2-Crtap-3'UTR
Plasmid#61772PurposeLuciferase reporter containing the 3'UTR of mouse CrtapDepositorInsertmouse Crtap 3'UTR
UseLuciferaseAvailable SinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFOSGE_DUM1B01
Plasmid#71089PurposeGateway entry cloneDepositorInsertClk (Clk Fly)
UseGateway entry vectorAvailable SinceFeb. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK278
Plasmid#71134PurposeExpression of Acetobacter aceti 1023 PurE (H89N mutant) in E. coliDepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
ExpressionBacterialMutationchanges histidine-89 to asparagine; additional N-…PromoterT7Available SinceFeb. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK130
Plasmid#71129PurposeExpression of Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide synthetase (PurK) in E. coliDepositorInsertN5-carboxyaminoimidazole ribonucleotide synthetase
TagsHis6ExpressionBacterialMutationchanged valine-319 to phenylalaninePromoterT7Available SinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK173
Plasmid#71831PurposeCloned region contains Acetobacter aceti 1023 hypothetical protein AZ09_02685, purE, and purK genesDepositorInsertshypothetical protein
N5-carboxyaminoimidazole ribonucleotide mutase
N5-carboxyaminoimidazole ribonucleotide synthetase
ExpressionBacterialAvailable SinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFOSGE_COF2C10
Plasmid#70927PurposeGateway entry cloneDepositorInsertokr (okr Fly)
UseGateway entry vectorAvailable SinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK320
Plasmid#71144PurposeExpression of Acetobacter aceti 1023 PurE (H59S mutant) in E. coliDepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
ExpressionBacterialMutationchanges histidine-59 to serine; additional N-term…PromoterT7Available SinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK319
Plasmid#71143PurposeExpression of Acetobacter aceti 1023 PurE (H59A mutant) in E. coliDepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
ExpressionBacterialMutationchanges histidine-59 to alanine; additional N-ter…PromoterT7Available SinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK318
Plasmid#71142PurposeExpression of Acetobacter aceti 1023 PurE (H59Q mutant) in E. coliDepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
ExpressionBacterialMutationchanges histidine-59 to glutamine; additional N-t…PromoterT7Available SinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK315
Plasmid#71141PurposeExpression of Acetobacter aceti 1023 PurE (H89G mutant) in E. coliDepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
ExpressionBacterialMutationchanges histidine-89 to glycine; additional N-ter…PromoterT7Available SinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK314
Plasmid#71140PurposeExpression of Acetobacter aceti 1023 PurE (H89V mutant) in E. coliDepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
ExpressionBacterialMutationchanges histidine-89 to valine; additional N-term…PromoterT7Available SinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK174
Plasmid#71835PurposeExpression of Acetobacter aceti 1023 PurE and PurK in E. coliDepositorInsertsN5-carboxyaminoimidazole ribonucleotide mutase
N5-carboxyaminoimidazole ribonucleotide synthetase
ExpressionBacterialMutationadditional N-terminal methionine encoded by this …PromoterT7 and endogenousAvailable SinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only