We narrowed to 2,342 results for: puc19

1. pUC19_mRuby-3xFLAG-NLS-3xSV40-poly(A)_loxP-PGK-Puro-loxP

   Plasmid

   #195505

   Purpose

   T-REX17 donor plasmid to generate locus specific integration of mRuby followed by 3xSV40 poly(A) into Exon 1 of human lncRNA T-REX17

   Depositor

   Alexander Meissner

   Article

   Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

   Insert

   pT-REX-mRuby-3xFLAG-NLS-3xSV40-poly(A)_loxP-PGK-Puro-loxP_T-REX(Ex1)
   

   Use

   CRISPR; Donor vector

   Tags

   mRuby-3xFLAG-NLS-3xSV40-poly(A)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC19-MAFA-PQR-NLS-tdTomato-P2A-puro-PGK-puro

   Plasmid

   #121174

   Purpose

   Donor template for CRISPR-Cas9 mediated generation of MAFA-NLS-tdTomato reporter cell lines

   Depositor

   Timo Otonkoski

   Insert

   MAFA-PQR-NLS-tdTomato-P2A-Puro-PGK-Puro
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC19-GCG-T2A-NLS-EmGFP-P2A-puro-PGK-puro

   Plasmid

   #121175

   Purpose

   Donor template for CRISPR-Cas9 mediated generation of GCG-NLS-EmGFP reporter cell lines

   Depositor

   Timo Otonkoski

   Insert

   GCG-T2A-NLS-EmGFP-P2A-Puro-PGK-Puro
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. JM109

   Bacterial Strain

   #49761

   Purpose

   E.coli K-12 derivative

   Depositor

   Joachim Messing

   Article

   Yanisch-Perron et al Gene. 1985;33(1):103-19.

   Bacterial Resistance

   None

   Available since

   Sept. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

5. JM106

   Bacterial Strain

   #49757

   Purpose

   E.coli K-12 derivative

   Depositor

   Joachim Messing

   Article

   Yanisch-Perron et al Gene. 1985;33(1):103-19.

   Bacterial Resistance

   None

   Species

   Escherichia coli

   Available since

   Sept. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pFloxed DHFR-TS*

   Plasmid

   #100605

   Purpose

   pUC19 with loxP-pDHFR-TS:DHFR-TS*(Pyr-R)-Stop-loxP-DHFR-TS 3' UTR

   Depositor

   David Sibley

   Insert

   Floxed TgDHFR-TS*
   

   Use

   Toxoplasma expression

   Tags

   Expression

   Mutation

   Pyrimethamine resistance

   Promoter

   Available since

   Sept. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. JM107

   Bacterial Strain

   #49759

   Purpose

   E.coli K-12 derivative

   Depositor

   Joachim Messing

   Article

   Yanisch-Perron et al Gene. 1985;33(1):103-19.

   Bacterial Resistance

   None

   Species

   Escherichia coli

   Available since

   Sept. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

8. JM110

   Bacterial Strain

   #49763

   Purpose

   E.coli K-12 derivative

   Depositor

   Joachim Messing

   Article

   Yanisch-Perron et al Gene. 1985;33(1):103-19.

   Bacterial Resistance

   Streptomycin

   Available since

   Oct. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pUC-ODN

   Plasmid

   #208642

   Purpose

   Empty vector for long single strand DNA (up to 1.5k) preparation. This plasmid is a pUC19 with 6 point mutations that remove BspQI, BtsI, BsrDI sites. Mutations adopted from Patent WO/2018/015995.

   Depositor

   Quanfu Ma

   Type

   Empty backbone

   Use

   Long single strand dna production

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCRISPR

    Plasmid

    #113252

    Purpose

    pUC19 backbone with E. coli CRISPR array

    Depositor

    Jennifer Doudna

    Article

    Wright et al Nat Struct Mol Biol. 2016 Oct;23(10):876

    Insert

    E. coli CRISPR array
    

    Use

    Integration substrate

    Tags

    Expression

    Mutation

    Promoter

    NA

    Available since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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